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1.
J Opt Soc Am A Opt Image Sci Vis ; 17(12): 2229-35, 2000 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-11140482

RESUMO

We apply functional analysis to the scattered electromagnetic field of a particle with spherical symmetry to obtain a pair of integral transforms for converting the Mie-scattering amplitudes S perpendicular (theta) and S parallel (theta) into the Mie coefficients an and bn. In the case of a homogeneous sphere, a simple mathematical construction is derived that uniquely inverts the Mie coefficients to find the refractive index and the radius of the particle. A more general method for construction of the refractive-index profile of an arbitrary sphere is discussed that follows from the treatment of Newton and Sabatier.

2.
Opt Lett ; 25(24): 1792-4, 2000 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-18066346

RESUMO

A family of closed-form expressions for the scalar field of strongly focused Gaussian beams in oblate spheroidal coordinates is given. The solutions satisfy the wave equation and are free from singularities. The lowest-order solution in the far field closely matches the energy density produced by a sine-condition, high-aperture lens illuminated by a paraxial Gaussian beam. At the large waist limit the solution reduces to the paraxial Gaussian beam form. The solution is equivalent to the spherical wave of a combined complex point source and sink but has the advantage of being more directly interpretatable.

3.
Appl Opt ; 37(18): 4027-33, 1998 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-18273375

RESUMO

A new approach suitable for solving inverse problems in multiangle light scattering is presented. The method takes advantage of multidimensional function approximation capability of radial basis function neural networks. An algorithm for training the networks is described in detail. It is shown that the radius and refractive index of homogeneous spheres can be recovered accurately and quickly, with maximum relative errors of the order of 10(-3) and mean errors as low as 10(-5). The influence of the angular range of available scattering data on the loss of information and inversion accuracy is investigated, and it is shown that more than two thirds of input data can be removed before substantial degradation of accuracy occurs.

4.
Appl Environ Microbiol ; 62(8): 3073, 1996 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-16535391

RESUMO

Volume 62, no. 5, p. 1702, column 2, equation 3: the equation should read as follows. g(sup1)((tau)) = [g(sup2)((tau)) - 1](sup1/2) = exp[-K(sup2)(D(inf1) cos(sup2)(alpha) + D(inf2) sin(sup2)(alpha))(tau)] (3) [This corrects the article on p. 1699 in vol. 62.].

5.
Appl Environ Microbiol ; 62(5): 1699-704, 1996 May.
Artigo em Inglês | MEDLINE | ID: mdl-16535316

RESUMO

Quasielastic light scattering (QLS) and laser diffractometry (LD) are relatively novel nondestructive procedures for estimating the sizes of bacterial spores in suspension. This study for the first time directly compared the two with a destructive procedure, namely, scanning electron microscopy (SEM), for quasispherical spores of Bacillus sphaericus. Because of the different physical aspect measured, the sizes derived by QLS and LD are, as could be expected for spores with an exosporium, significantly different. The larger estimates obtained by QLS (1.70, 1.58, and 1.14 (mu)m for spores produced at 15(deg)C [BS15], 20(deg)C [BS20], and 30(deg)C [BS30], respectively) than by LD (0.56 [BS15], 0.58 [BS20], and 0.52 [BS30] (mu)m) and SEM (0.64 [BS15], 0.58 [BS20], and 0.70 [BS30] (mu)m) are explained in terms of the detection by QLS, LD, and SEM of different spore layers and the degree of nonsphericity of the latter.

6.
J Appl Bacteriol ; 74(5): 578-82, 1993 May.
Artigo em Inglês | MEDLINE | ID: mdl-8486564

RESUMO

The distribution of water in the protoplast and integument of three populations of Bacillus sphaericus spores was determined by laser diffractometry together with the sizes of their integuments and protoplasts. The spores were produced at 15, 20 and 30 degrees C. Spores grown at 15 degrees C had protoplast and integument water contents similar to those produced at 20 degrees C, while those grown at 30 degrees C had significantly lower water contents than the other two populations. The inner (or protoplast) radii of the spores produced at 15, 20 and 30 degrees C were 0.41 +/- 0.02 microns, 0.42 +/- 0.01 microns and 0.38 +/- 0.02 microns whilst the outer (or whole spore) radii were 0.56 +/- 0.01 microns, 0.58 +/- 0.01 microns and 0.52 +/- 0.02 microns respectively. The ratios of integument to protoplast radius were 0.40 +/- 0.02, 0.43 +/- 0.07 and 0.41 +/- 0.03 respectively.


Assuntos
Bacillus/química , Esporos Bacterianos/química , Água/análise , Bacillus/fisiologia , Bacillus/ultraestrutura , Temperatura Alta , Lasers , Microscopia Eletrônica de Varredura , Espalhamento de Radiação , Esporos Bacterianos/fisiologia , Esporos Bacterianos/ultraestrutura
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