[Reactive oxygen forms and Ca ions as possible intermediaries under the induction of heat resistance of plant cells by jasmonic acid].

The participation of reactive oxygen species (ROS) and calcium ions in realization of influence of exogenous jasmonic acid (JA) on the heat resistance of wheat coleoptiles has been investigated. Influence of 1 microM JA caused the transitional intensifying of generation of superoxide anion-radical (O2*-) and hydrogen peroxide in coleoptiles with the maximum within 15-30 minutes after the treatment beginning. Within the first hour after the beginning of coleoptiles treatment with JA the increase of superoxide dismutase (SOD) activity was noted. Later on (within 5-24 hours after the treatment beginning) there was the lowering of ROS generation by coleoptiles of experimental variant, and the SOD activity approached the control value. Intensifying of generation of superoxide radical induced by JA was suppressed by the antioxidant ionol and was partially levelled by imidazole (inhibitor of NADPH-oxidase), EGTA (chelator of extracellular calcium) and lanthanum chloride (calcium channels blocker). Pretreatment of coleoptiles with the ionol, imidazole, EGTA and LaC3l3 also partially removed the effect of increase of their resistance to the damaging heating caused by exogenous JA. It is supposed that the ROS generated with participation NADPH-oxidase, which activity depends on the receipt of calcium ions from extracellular space in the cytosol, are involved in realization of physiological effects of JA.

[On a problem of syphilitic damage of the eye].

Clinical features and epidemiology of syphilitic damage of the eye in Smolensk region was studied in 1996-2008 period. 32 medical charts of patients with ophthalmosyphilis were analyzed. Ophthalmosyphilis is usually registered in patients with secondary syphilis with disease duration more than 6 months (56.3%) and in patients with late neurosyphilis (40.6%). The diagnosis was established in 24.5+4.8 (from 14 till 34) days after clinical presentation. Clinical forms of ophthalmosyphilis included bilateral uveitis (15.6%), optic neuritis (15.8%), iridocyclitis (12.5%) and choreoretinitis (12.5%). Complications of ophthalmosyphilis included optic nerve atrophy developed in 6.2%, cataract--6.2% and glaucoma--3.1%. In epidemiologically unfavorable situation caution should be taken by ophthalmologists in terms of syphilitic infection, appropriate laboratory tests should be considered.