RESUMO
Objective:To evaluate the measurement uncertainty in the determination of paracetamol tablets by UV. Methods:The mathematical model of content determination by UV was established and the uncertainty sources were analyzed. Each active component of uncertainty was calculated, and the expanded uncertainty was obtained. Results:The expanded uncertainty for the UV determination of paracetamol tablets was 1. 2%,and the content determination result was (97. 0 ± 1. 2) % (k=2). Conclusion:The main sources of uncertainty are analyzed, which can provide reliable theoretical basis for the effective control of the method.
RESUMO
Objective:To establish an HPLC method for the determination of two components and the preservative in compound dextromethorphan hydrobromide syrups. Methods:An Agilent Zobax SB-C18 column(250 mm × 4. 6 mm,5 μm) was used with meth-anesulfonic acid solution (adding 4. 8g methanesulfonic acid and 10ml triethylamine into 750ml water,and adjusting the pH value to 3. 5 by phosphoric acid)-acetonitrile (75∶25) as the mobile phase at the flow rate of 1. 0 ml·min-1 and 280nm as the detection wave-length. Results:The calibration curve was linear within the range of 102-1 025μg·ml-1 for guaifenesin,15-619μg ·ml-1 for dextro-methorphan hydrobromide and 10-407μg·ml-1 for benzoic acid. The average recovery of guaifenesin, dextromethorphan hydrobromide and benzoic acid was 100. 0% (RSD=0. 35%), 100. 1%(RSD=0. 77%)and 100. 8%(RSD=0. 49%), respectively. Conclusion:The method is simple,rapid and accurate,and suitable for the quality assessment of compound dextromethorphan hydrobromide syrups.
