Oocyte structure and ultrastructure in the Mexican silverside fish Chirostoma humboldtianum (Atheriniformes: Atherinopsidae).

The structural and ultrastructural features of gonads from endemic Mexican fish have received scarce attention. This study describes the histological and ultrastructural characteristics of the oocyte in Chirostoma humboldtianum. The ovary is asynchronic, and as such, most phases of oocyte development are found in the same ovary. The complete process of oogenesis was divided in five stages: oogonium and folliculogenesis, primary growth, cortical alveoli and lipid inclusions, vitellogenesis and maturation. The presence of big filaments, which appear at the end of primary growth, induces some common follicular adaptation. During primary growth, abundant ribosomes, rough endoplasmic reticulum, and mitochondria are grouped in the cytoplasm. At the end of this stage, the Z1 layer of the chorion is developed, while microvilli start to be evident as well. In the cortical alveoli and lipid droplets phase, intense PAS positive vesicles, some of them containing nucleoid material, are observed in the peripheral cytoplasm and the lipid droplets take a more central position. In vitellogenesis, the proteic yolk accumulates in a centripetal way while the chorion is completely formed. In maturation, the germinal vesicle migrates to the animal pole, meiosis is restored, and there is nuclear breakdown. The oocyte increases its size and holds some oil droplets and a big fluid mass of yolk. On the outside, filaments surround the oocyte completely.

Tobacco use amongst nursing and physiotherapy students: a cross sectional questionnaire survey.

AIM: The aim of this research was to evaluate the prevalence, knowledge and attitudes of tobacco use in the students of the School of Health Sciences in the Campus of Ponferrada (University of León, Spain). Two-hundred and sixty-six (88.3% of respondents) students were assessed by a confidential questionnaire, which was distributed, completed by the students and collected in the same session. METHODS: This was a descriptive quantitative survey research design to examine the tobacco use among nursing and physiotherapy students. RESULTS: The global prevalence was 29.3%. In relation to academic courses we have observed statistical significance (p=0.006). Therefore students increased tobacco use while their training at the school. Seventy (89.7%) of students were smokers prior to commencing degree. Nursing students declared were unfamiliar with strategies and methods to help people to quit. The first academic course students (78.6%) significantly differed (p=0.009) from second and third course. We have found scores statistically significant (p=0.04) about the students opinion about health professionals social role function. CONCLUSION: Tobacco consumption in university health students in the third year was higher than people aged 16-24 years old reported from the National Health Survey. The findings showed a great need to improve the curricular content in the area of tobacco including information, prevention, and treatment.

Asian Americans and cancer clinical trials: a mixed-methods approach to understanding awareness and experience.

Cancer clinical trials have been based on low accrual rates. Barriers to recruitment of minority populations affect the generalizability and impact of trial findings for those populations. The authors undertook a mixed-methods approach to understanding levels of awareness and experiences with cancer clinical trials. A survey was administered to new cancer patients and their caretakers (family, close friends, or other social support) at outpatient oncology clinics. Field observations of the trial accrual process also were conducted by employing the grounded theory approach in qualitative methods. Comparison of survey results for Asian-American respondents and non-Asian respondents indicated that Asians were less likely to have heard the term "clinical trial" and were more likely to define a clinical trial as "an experiment" or "a test procedure in a clinic" than non-Asians. Asians were more likely to have employer-based insurance and to report understanding issues related to cost reimbursement. Asians were less likely to have been involved in or to know someone in a trial and reported less willingness than white respondents to consider trial participation. Qualitative observations suggested that Asians who presented for a potential trial were interested in the availability of a novel cancer therapy but were not eligible for available trials. Multiple strategies will be necessary to enhance awareness of and experience with accrual to cancer clinical trials for Asians, including richer understanding and increased involvement of Asians in cancer clinical trials and greater attention to the location and diversity of the Asian population in structuring study centers and evaluating trial results.