[Prevalence of cardiovascular diseases in organized groups of employees and results of long-term multifactorial prevention]. / Dinamika rasprostranennosti serdechno-sosudistykh zabolevanii i rezul'taty mnogofaktornoi profilaktiki pri mnogoletnikh nabliudeniiakh v organizovannykh kollektivakh.

AIM: Evaluation of cardiovascular diseases (CVD) prevalence and their dynamics after active prevention program. MATERIALS AND METHODS: 1382 male and 155 female volunteers were included into the group of active prevention in their office. 1024 males and 776 females with natural history were compared as a control group. 5-year follow-up was performed in 543 males and 569 females in comparison with 97 males and 119 females. 10-year follow-up covered 82 males and 191 females from the active prevention group and 178 males and 118 females from the referent group. RESULTS: The risk factor correction has resulted in a significant decrease in both systolic and diastolic BP mean levels, prevalence of ECG abnormalities, smoking habits, overweight and combination of the risk factors, general mortality rate, CHD and stroke mortality, number of days of temporary disability. CONCLUSION: The primary and secondary "in office" prevention for 5 to 10 years diminishes the prevalence of the risk factors. The temporary and primary disability rates were connected with the presence of CVD. CVD, in line with other risk factors, significantly increased cardiovascular and general mortality rates.

[Long-term study of the prevalence of arterial hypertension and outcomes of multifactorial prophylaxis in organized groups of employees]. / Dinamika pasprostranennosti arterial'noi gipertonii i rezul'taty mnogofaktornoi profilaktiki pri mnogoletnikh nabliudeniiakh sredi rabochikh i sluzhashchikh organizovannykh kollektivov.

AIM: To evaluate trends in arterial hypertension (AH) and other risk factors and their changes after active prevention program. MATERIAL AND METHODS: 1382 male and 1555 female volunteers were included into the group of active prevention at the workplace vs 1024 male and 776 female controls. 5-year follow-up covered 543 males and 569 females from the prevention group against 97 male and 119 female controls. 10-year follow-up covered 82 males and 191 females, 178 males and 118 females, respectively. RESULTS: Risk factor correction produced a significant decrease in both systolic and diastolic BP levels, prevalence of ECG deteriorations, smoking habits occurrence, overweight. Overall mortality rate, CHD and stroke mortality were significantly less frequent in the prevention group. This group had also less days of temporary disability. CONCLUSION: The primary and secondary "in office" prevention for 5 to 10 years diminishes prevalence of risk factors. The temporary disability rate depends on the presence of arterial hypertension. The latter together with the other main risk facts significantly influences cardiovascular and general mortality rates.

[Expression of the gene for transforming growth factor type alpha, transferred into A431 cells by a recombinant retroviral vector]. / Ekspressiia gena transformiruiushchego faktora rosta tipa alpha, perenesennogo rekombinantnym retrovirusnym vektorom v kletki A431.

Cells of human epidermoid carcinoma A431 were used for obtaining of the cell line constantly expressing TGF-alpha. Recombinant virions were obtained by introducing the proviral DNA into PA317 cells by means of electrotransfection. A protein with the EGF-competing activity was found in a conditioned media of the chosen clone A431/1522-4. The concentration of this protein was several times higher than in a conditioned media of wild type A431 cells. By means of electrophoresis, isoelectric focusing, and immunochemical analysis it was shown that the protein is TGF-alpha. Similarly to EGF, the extracted TGF-alpha entirely displaced 125I-EGF specifically bound to receptor. TGF-alpha produced by the A431/1522 cells also stimulated autophosphorylation of the EGF receptor.