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1.
Sci Rep ; 5: 9380, 2015 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-25797885

RESUMO

To preserve environmental and human health, improved treatment processes are needed to reduce nutrients, microbes, and emerging chemical contaminants from domestic wastewater prior to discharge into the environment. Electrocoagulation (EC) treatment is increasingly used to treat industrial wastewater; however, this technology has not yet been thoroughly assessed for its potential to reduce concentrations of nutrients, a variety of microbial surrogates, and personal care products found in domestic wastewater. This investigation's objective was to determine the efficiency of a benchtop EC unit with aluminum sacrificial electrodes to reduce concentrations of the aforementioned biological and chemical pollutants from raw and tertiary-treated domestic wastewater. EC treatment resulted in significant reductions (p < 0.05, α = 0.05) in phosphate, all microbial surrogates, and several personal care products from raw and tertiary-treated domestic wastewater. When wastewater was augmented with microbial surrogates representing bacterial, viral, and protozoan pathogens to measure the extent of reduction, EC treatment resulted in up to 7-log10 reduction of microbial surrogates. Future pilot and full-scale investigations are needed to optimize EC treatment for the following: reducing nitrogen species, personal care products, and energy consumption; elucidating the mechanisms behind microbial reductions; and performing life cycle analyses to determine the appropriateness of implementation.


Assuntos
Técnicas Eletroquímicas , Águas Residuárias , Poluentes Químicos da Água/isolamento & purificação , Purificação da Água/métodos , Alumínio , Bacillus subtilis/isolamento & purificação , Eletrodos , Enterococcus faecalis/isolamento & purificação , Desenho de Equipamento , Escherichia coli/isolamento & purificação , Humanos , Nitrogênio/química , Nitrogênio/isolamento & purificação , Fosfatos/química , Fosfatos/isolamento & purificação , Águas Residuárias/química , Águas Residuárias/microbiologia , Purificação da Água/instrumentação
2.
Water Res ; 65: 257-70, 2014 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-25129566

RESUMO

Wastewater treatment ponds (WTP) are one of the most widespread treatment technologies in the world; however, the mechanisms and extent of enteric virus removal in these systems are poorly understood. Two WTP systems in Bolivia, with similar overall hydraulic retention times but different first stages of treatment, were analyzed for enteric virus removal. One system consisted of a facultative pond followed by two maturation ponds (three-pond system) and the other consisted of an upflow anaerobic sludge blanket (UASB) reactor followed by two maturation (polishing) ponds (UASB-pond system). Quantitative polymerase chain reaction with reverse transcription (RT-qPCR) was used to measure concentrations of norovirus, rotavirus, and pepper mild mottle virus, while cell culture methods were used to measure concentrations of culturable enteroviruses (EV). Limited virus removal was observed with RT-qPCR in either system; however, the three-pond system removed culturable EV with greater efficiency than the UASB-pond system. The majority of viruses were not associated with particles and only a small proportion was associated with particles larger than 180 µm; thus, it is unlikely that sedimentation is a major mechanism of virus removal. High concentrations of viruses were associated with particles between 0.45 and 180 µm in the UASB reactor effluent, but not in the facultative pond effluent. The association of viruses with this size class of particles may explain why only minimal virus removal was observed in the UASB-pond system. Quantitative microbial risk assessment of the treated effluent for reuse for restricted irrigation indicated that the three-pond system effluent requires an additional 1- to 2-log10 reduction of viruses to achieve the WHO health target of <10(-4) disability-adjusted life years (DALYs) lost per person per year; however, the UASB-pond system effluent may require an additional 2.5- to 4.5-log10 reduction of viruses.


Assuntos
Enterovirus/isolamento & purificação , Eliminação de Resíduos Líquidos/métodos , Águas Residuárias/virologia , Purificação da Água/métodos , Irrigação Agrícola , Animais , Bolívia , Linhagem Celular , Chlorocebus aethiops , Enterovirus/genética , Tamanho da Partícula , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Medição de Risco , Abastecimento de Água
3.
Water Environ Res ; 73(6): 711-20, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-11833765

RESUMO

The Upper Occoquan Sewage Authority (UOSA) Water Reclamation Plant, Centreville, Virginia, is a state-of-the-art wastewater treatment plant that was created to treat area wastewater and provide protection for the Occoquan Reservoir. This study investigated UOSA's unit processes as barriers to pathogenic as well as altemative and traditional-indicator microorganisms. Samples were collected once a month for 1 year from eight sites within UOSA's advanced wastewater reclamation plant. The eight sites were monitored for indicator bacteria total and fecal coliforms, enterococci, Clostridium, coliphage (the virus that infects Escherichia coli), human enteroviruses, and enteric protozoa. Overall, the plant was able to achieve a 5- to 7-log10 reduction of bacteria, 5-log10 reduction of enteroviruses, 4-log10 reduction for Clostridium, and 4.6-log10 reduction of protozoa. Total coliforms, enterococci, Clostridium, coliphage, Cryptosporidium, and Giardia were all detected in four or fewer samples of the final effluent. No enteroviruses or fecal coliforms were detected in the final effluent. The microbiological quality of reclaimed water and the reservoir water were compared. In every case, the treated wastewater was of a better quality than the ambient water in the reservoir, thus indicating that the reclaimed water will not adversely affect the water quality for downstream users.


Assuntos
Monitoramento Ambiental , Esgotos/microbiologia , Microbiologia da Água , Purificação da Água/métodos , Animais , Clostridium/isolamento & purificação , Conservação dos Recursos Naturais , Cryptosporidium/isolamento & purificação , Enterobacteriaceae/isolamento & purificação , Giardia/isolamento & purificação , Humanos , Saúde Pública , Controle de Qualidade
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