Positive association between SRA1 rs801460 variant and proliferative type of benign breast disease with atypia in Ukrainian females.

AIM: To investigate the association between SRA1 rs801460 and rs10463297 variants and proliferative type of benign breast disease with atypia development in Ukrainian females. MATERIALS AND METHODS: 83 individuals diagnosed with proliferative type of benign breast disease with atypia and 115 without atypia were enrolled in the study. The rs801460 and rs10463297 variants genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism analysis. Hematoxylin and eosin, toluidine blue and van Gieson's picrofuchsin methods were used for sections staining. RESULTS: It was revealed that SRA1 rs801460-variant is associated with proliferative type of benign breast disease with atypia development both before and after adjustment for risk factors (age, body mass index, age of menarche, oral contraceptives intake and burdened history of breast cancer). The risk for mentioned disease in the individuals with rs801460 TT-genotype is 2.2 times higher (confidence interval 1.010-4.800; p = 0.047) than in individuals with the CC and CT genotypes. No link between SRA1 rs10463297 and proliferative type of benign breast disease with atypia occurrence in Ukrainian females was found. CONCLUSION: The present study specified that SRA1 rs801460, but not rs10463297, can be the strong genetic predictor for benign breast disease with atypia in Ukrainian females.

[LASER-INDUCED SCLEROTHERAPY OF MAMMARY GLAND CYSTS].

Own experience of the patients treatment for the mammary gland cysts (MGC) in its benign dysplasia was analyzed. Comparative analysis of efficacy of a laser-induced sclerotherapy for the MGC and chemical sclerosing (96% еthanol) was conducted. There was established, that the cyst recurrency rate depends upon volume of the cavity and it is reducing while application of a laser-induced sclerotherapy.

[Immediate and long-term results of surgical treatment of benign breast disease].

Sector resection of the breast is the standard surgical treatment of benign breast disease, but often it has unsatisfactory outcomes. The aim of the study was a comparative analysis of the effectiveness of surgical treatment of patients with benign breast diseases after sector resection and after modification of the procedure. Classical sector resection was performed on 45 women, 106 - underwent operations with using of plastic surgery elements. The effectiveness of treatment was defined by the presence of complications in the early postoperative period; the quality of life was studied using questionnaires--The Short Form Medical Study (SF-36) and The Breast Evaluation Questionnaire (BEQ)--before surgery and after 3 and 12 months after surgery. After sector resection was 44% of the complications in the early postoperative period, after the developed techniques - 17%. 3 months after the operation quality of life and aesthetic satisfaction of breast in patients of both groups decreased. 1 year after sector resection the rates of satisfaction of breast aesthetic appearance were significantly lower than the preoperative level. In patients after modified operations at 1 year was observed a significant improvement in well-being and satisfaction of aesthetic appearance of breast. The method of sector resection has a greater number of complications; it leads to deterioration of the aesthetic appearance of the breast in 1 year after surgery. Surgery techniques with elements of mammaplasty have fewer complications and improve quality of life after surgery.

[The clinical significance of pvuii polymorphism estradiol receptor alpha gene to improve diagnosis of proliferative forms of benign breast dysplasia].

The aim - to determine the role of single nucleotide polymorphism PvuII of the gene EsRα as an indicator of proliferative activity in benign breast dysplasia (BBD) and its effect on receptor status of breast tissue for expression EsRα. Defined genotype PvuII polymorphism EsRα, the expression level in remote EsRα among patients with BBD. For these patients, and morphological parameters samples are divided into groups and compared. It is shown that there is a connection between the PvuII-gene polymorphism EsRα with the degree of proliferation (χ2=43,142; p<0,0001) and the expression level EsRα (χ2=51,041; p<0,0001) in breast tissue at BBD in patients with homozygous (C/C) polymorphism PvuІІ of the gene EsRα. Addition to the standard morphological study justified immunohistochemical study with the definition of the expression level of EsRα due to the fact that the increase in the level of expression associated with an increase in cell proliferation in tumors with BBD (χ2=7,370; p=0,007). An algorithm for the diagnosis of proliferative forms BBD.