RESUMO
We have studied the prophylactic effect of new derivatives of ursodeoxycholic acid (UDCA), including UDCA-N-acetylcysteine, UDCA-L-acetylcysteine, and nor-UDCA (in doses equivalent to 40 mg/kg of UDCA) on the development of experimental alcoholic steatohepatitis induced by the Lieber-DeCarli liquid ethanol-containing diet. Results demonstrated that most of the investigated compounds produced a hepatoprotective effect, improving biochemical tests and liver morphology, as manifested by decreasing steatosis intensity, activity of alkaline phosphatase and gamma-glutamyltranspeptidase, triglyceride level in blood serum and liver, and TNF alpha content. However, nor-UDCA was most effective as compared to UDCA in preventing the accumulation of triglycerides in the liver.
Assuntos
Colagogos e Coleréticos/farmacologia , Fígado Gorduroso Alcoólico/prevenção & controle , Fígado/efeitos dos fármacos , Substâncias Protetoras/farmacologia , Ácido Ursodesoxicólico/análogos & derivados , Ácido Ursodesoxicólico/farmacologia , Acetilcisteína/química , Fosfatase Alcalina/metabolismo , Animais , Dieta , Etanol , Fígado Gorduroso Alcoólico/tratamento farmacológico , Fígado Gorduroso Alcoólico/metabolismo , Fígado Gorduroso Alcoólico/patologia , Feminino , Fígado/metabolismo , Ratos , Ratos Wistar , Triglicerídeos/metabolismo , Fator de Necrose Tumoral alfa/sangue , gama-Glutamiltransferase/metabolismoRESUMO
The effect of infliximab (1 and 10 mg/kg, i.p., during 10 days) on alcoholic steatohepatitis has been studied in rats fed with ethanol-containing liquid diet over 10 weeks. Both doses of infliximab showed immunomodulatory and anti-inflammatory effects, whereas only the maximum drug dose significantly decreased lipid accumulation in the liver. It can be concluded that infliximab (10 mg/kg) acts as an effective hepatoprotector, anti-inflammatory agent, and immunomodulator in rats with experimental alcoholic steatohepatitis.
Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Anticorpos Monoclonais/farmacologia , Fígado Gorduroso Alcoólico/tratamento farmacológico , Fatores Imunológicos/farmacologia , Peroxidação de Lipídeos/efeitos dos fármacos , Animais , Depressores do Sistema Nervoso Central/efeitos adversos , Depressores do Sistema Nervoso Central/farmacologia , Modelos Animais de Doenças , Etanol/efeitos adversos , Etanol/farmacologia , Fígado Gorduroso Alcoólico/sangue , Fígado Gorduroso Alcoólico/etiologia , Feminino , Infliximab , Ratos , Ratos WistarRESUMO
White rats received rations with varying content of linoleic acid (3.17 and 90 g/kg of food) during 30 days. The control rats that received food with low content of linoleic acid showed a higher content of triglycerides in the liver. Elevation of linoleic acid level in the ration of animals with chronic alcoholic intoxication diminishes the symptoms of alcoholic hepatosis: hepatocyte vacuolization and the content of neutral lipids in them are lowered, serum enzyme (alanine-aminotransferase, gamma-glutamyltransferase) activity decreases as well as the signs of essential fatty acid deficiency disappear.
