RESUMO
This paper deals with the possibility of application of aluminum phthalocyanine (AlPc) nanoparticles in clinical practice. AlPc fluoresces in the molecular form but in the form of nanoparticles it does not. Separation of molecules from an AlPc nanoparticle and therefore the appearance of fluorescence occurs under the effect of a number of biochemo-physical factors. Owing to this feature the application of AlPc nanoparticles followed by the measurement of fluorescence spectra is proposed as a diagnostics method. It was shown that after AlPc nanoparticle application on a tooth surface the fluorescence intensity in the enamel microdamage area is 2-3 times higher than that in the normal enamel area. The appearance of fluorescence after application of AlPc nanoparticles on skin autografts testifies to the presence of inflammation.
Assuntos
Esmalte Dentário/patologia , Odontologia/métodos , Rejeição de Enxerto/diagnóstico , Indóis , Nanopartículas Metálicas , Compostos Organometálicos , Transplante de Pele , Animais , Coloides/química , Procedimentos Cirúrgicos Dermatológicos , Feminino , Fluorescência , Humanos , Técnicas In Vitro , Indóis/química , Inflamação/diagnóstico , Luz , Nanopartículas Metálicas/química , Camundongos , Compostos Organometálicos/química , Fatores de Risco , Espalhamento de Radiação , Pele/patologia , Soluções , Espectrometria de Fluorescência , Fatores de Tempo , Transplante AutólogoRESUMO
We show that iron(II)-phthalocyanines are able to catalyze guanosine oxidation by molecular oxygen in the presence of reducing agents such as ascorbic acid and 2-mercaptoethanol. The products of 5'-monophosphate-2'-deoxyguanosine (dGMP) oxidation were directly analyzed using the HPLC-ESI/MS method. The main oxidation products were 5'-phospho-2'-deoxy-8-oxo-7,8-dihydroguanine and the 1,N2-glyoxal adduct of the 5'-monophosphate-2'-deoxyguanosine.
