RESUMO
The identification and the development of potential growth factors for the cells of the periodontal ligament is an important and novel stratagem for the regeneration of tissue destroyed by periodontal pathogens. The problem is that growth factors are difficult to synthesize, very immunogenic and expensive. The purpose of our study was to determine if angiotensin-II, a naturally occurring octo-peptide with growth-factor like properties in cardiac muscle, could also stimulate cultured human periodontal ligament cells. If angiotensin-II does have growth factor properties, then it should be able to increase cytoplasmic calcium (a secondary messenger associated with mitogenesis) and to increase fibroblast proliferation. Fura-2 fluorescence and aequorin photometry were used to follow changes in cytoplasmic calcium. Cytometry and total amount of DNA were used to measure proliferation evoked by angiotensin-II. We found that angiotensin-II increased cytoplasmic calcium, the total amount of DNA and cell number, suggesting that angiotensin-II has mitogenic properties for cells isolated from human periodontal ligament.
Assuntos
Angiotensina II/farmacologia , Cálcio/metabolismo , Citoplasma/efeitos dos fármacos , DNA/efeitos dos fármacos , Substâncias de Crescimento/farmacologia , Mitógenos/farmacologia , Ligamento Periodontal/efeitos dos fármacos , Equorina , Contagem de Células/efeitos dos fármacos , Divisão Celular/efeitos dos fármacos , Células Cultivadas , Citoplasma/metabolismo , Fibroblastos/citologia , Fibroblastos/efeitos dos fármacos , Fibroblastos/metabolismo , Citometria de Fluxo , Corantes Fluorescentes , Fura-2 , Humanos , Proteínas Luminescentes , Ligamento Periodontal/citologia , Ligamento Periodontal/metabolismo , Fotometria , Sistemas do Segundo Mensageiro/efeitos dos fármacosRESUMO
A long-standing lesion associated with an undetected perforation of the furca in a mandibular molar was successfully managed using a multidisciplinary treatment approach. The sequence of therapeutic procedures involved conventional endodontic retreatment, an initial orthograde sealing of the perforation, guided tissue regeneration, and a subsequent orthograde resealing of the perforation with Ketac-Endo and Intermediate Restorative Material.
Assuntos
Perda do Osso Alveolar/cirurgia , Defeitos da Furca/cirurgia , Tratamento do Canal Radicular , Traumatismos Dentários/complicações , Raiz Dentária/lesões , Adulto , Feminino , Defeitos da Furca/etiologia , Cimentos de Ionômeros de Vidro , Regeneração Tecidual Guiada Periodontal , Guta-Percha , Humanos , Mandíbula , Metilmetacrilatos , Dente Molar , Equipe de Assistência ao Paciente , Bolsa Periodontal/etiologia , Bolsa Periodontal/cirurgia , Bolsa Periodontal/terapia , Reoperação , Materiais Restauradores do Canal Radicular , Tratamento do Canal Radicular/efeitos adversos , Tratamento do Canal Radicular/métodos , Traumatismos Dentários/etiologia , Traumatismos Dentários/terapia , Cimento de Óxido de Zinco e EugenolRESUMO
The free gingival graft is a simple, predictable technique for increasing the zone of attached gingiva. Dentists interested in performing minor surgical procedures should include the free gingival graft in their armamentarium. Indications and contraindications have been established, but the decision regarding the adequacy of the attached gingiva remains one of clinical judgment. The clinician must evaluate several variables, including the width of the attached gingiva, the patient's age, recession history, oral hygiene practices, teeth involved, and dental records. In the opinion of the authors, the free gingival graft usually should not be used in areas of inadequate attached gingiva when root coverage is indicated. Other techniques are more predictable and yield a more esthetic result.
