1.
In Vitro Cell Dev Biol Anim
; 31(10): 773-9, 1995 Nov.
Artigo
em Inglês
| MEDLINE
| ID: mdl-8564066
RESUMO
Conventional methods for isolating myogenic (satellite) cells are inadequate when only small quantities of muscle, the tissue in which satellite cells reside, are available. We have developed a tissue culture system that reliably permits isolation of intact, living, single muscle fibers with associated satellite cells from predominantly fast and slow muscles of rat and mouse; maintenance of the isolated fibers in vitro; dissociation, proliferation, and differentiation of satellite cells from each fiber; and removal of the fiber from culture for analysis.