RESUMO
A polysaccharide DOTP-80 from Dioscorea opposita Thunb was obtained by using the method of acid water-extraction and ethanol-precipitation. After being purified by chromatography, the structure characteristics of DOTP-80 were established. Based on the calibration curve obtained with standard dextrans, the molecular weight of the polysaccharide fraction DOTP-80 was calculated to be 123 kDa. The results of Infrared spectrum (FT-IR) indicated that the polysaccharide contained the α-configuration of sugar units. GC-MS analysis revealed that DOTP-80 was mainly composed of mannose and glucose. Alloxan-induced diabetic rats and mice models were developed to evaluate the in vivo hypoglycemic activity of the polysaccharide. The results indicated that a high dose DOTP-80 (400 mg/kg) had strong hypoglycemic activity. Moreover, DOTP-80 could increase the level of antioxidant enzymes (SOD) activity in alloxan-induced diabetic mice and stimulate an increase in glucose disposal in diabetic rats. Therefore, the polysaccharide DOTP-80 should be evaluated as a candidate for future studies on diabetes mellitus.
Assuntos
Dioscorea/química , Hipoglicemiantes/uso terapêutico , Raízes de Plantas/química , Polissacarídeos/uso terapêutico , Animais , Glicemia/efeitos dos fármacos , Diabetes Mellitus Experimental/tratamento farmacológicoRESUMO
A water-soluble polysaccharide from Sarcodia ceylonensis was obtained by using the method of water-extraction and ethanol-precipitation. The polysaccharide was further purified by chromatography on AB-8 and ADS-7 columns, yielding a pure polysaccharide termed SCP-60. The molecular weight (Mw) of SCP-60 was calculated to be 50.0 kDa, based on the calibration curve obtained with a series of Dextran T standards. The results of FT-IR indicated that the polysaccharide contains the α-configuration of sugar units. GC-MS analysis revealed that SCP-60 was mainly composed of galactose and glucose. NMR spectroscopy revealed SCP-60 had the backbone consisting of â 6)-α-Manp-(1 â, α-D-Glcp-(1 â, â 6)-α-D-Glcp-(1 â and â 6)-α-Galp-(1 â. In order to evaluate the antitumor activity in vivo of the polysaccharide, a sarcoma 180 model was used. The results showed SCP-60 had strong antitumor ability, meanwhile, SCP-60 at a high dose (100 mg/kg) could significantly increase the thymic and splenic indices of S180 mice, and strongly promote the secretion of IL-2, TNF-α and IFN-γ, increase the SOD activities and reduce the concentrations of MDA in blood. Therefore the polysaccharide SCP-60 should be explored as a novel potential antitumor drug.
Assuntos
Antineoplásicos/farmacologia , Polissacarídeos/farmacologia , Rodófitas/química , Animais , Antineoplásicos/química , Antineoplásicos/isolamento & purificação , Peso Corporal/efeitos dos fármacos , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Citocinas/biossíntese , Citocinas/sangue , Malondialdeído/metabolismo , Camundongos , Exsudatos de Plantas/química , Exsudatos de Plantas/farmacologia , Polissacarídeos/química , Polissacarídeos/isolamento & purificação , Superóxido Dismutase/metabolismo , Carga Tumoral/efeitos dos fármacosRESUMO
A water-soluble polysaccharide (BEBP) was extracted from Boletus edulis Bull using hot water extraction followed by ethanol precipitation. The polysaccharide BEBP was further purified by chromatography on a DEAE-cellulose column, giving three major polysaccharide fractions termed BEBP-1, BEBP-2 and BEBP-3. In the next experiment, the average molecular weight (Mw), IR and monosaccharide compositional analysis of the three polysaccharide fractions were determined. The evaluation of antioxidant activities both in vitro and in vivo suggested that BEBP-3 had good potential antioxidant activity, and should be explored as a novel potential antioxidant.
Assuntos
Antioxidantes/farmacologia , Basidiomycota/química , Polissacarídeos/isolamento & purificação , Polissacarídeos/farmacologia , Animais , Benzotiazóis , Fracionamento Químico , Cromatografia em Gel , Sequestradores de Radicais Livres/farmacologia , Radical Hidroxila/química , Malondialdeído/sangue , Camundongos , Monossacarídeos/análise , Oxirredução/efeitos dos fármacos , Espectroscopia de Infravermelho com Transformada de Fourier , Ácidos Sulfônicos/química , Superóxido Dismutase/metabolismo , Tiazóis/químicaRESUMO
The water-soluble crude polysaccharide (DDP) obtained from the aqueous extracts of the stem of Dendrobium denneanum through hot water extraction followed by ethanol precipitation, was found to have an average molecular weight (Mw) of about 484.7 kDa. Monosaccharide analysis revealed that DDP was composed of arabinose, xylose, mannose, glucose and galactose in a molar ratio of 1.00:2.66:8.92:34.20:10.16. The investigation of antioxidant activity both in vitro and in vivo showed that DDP is a potential antioxidant.
Assuntos
Antioxidantes/metabolismo , Dendrobium/química , Polissacarídeos/metabolismo , Água/química , Animais , Antioxidantes/química , Benzotiazóis/química , Benzotiazóis/metabolismo , Dendrobium/anatomia & histologia , Radical Hidroxila/química , Radical Hidroxila/metabolismo , Camundongos , Monossacarídeos/análise , Ressonância Magnética Nuclear Biomolecular , Extratos Vegetais/química , Extratos Vegetais/metabolismo , Caules de Planta/química , Polissacarídeos/química , Distribuição Aleatória , Espectroscopia de Infravermelho com Transformada de Fourier , Ácidos Sulfônicos/química , Ácidos Sulfônicos/metabolismo , Superóxido Dismutase/metabolismoRESUMO
With the incidence of type 2 diabetes mellitus increasing year after year, the technology of drug screening of type 2 diabetes mellitus progress rapidly, from the level of animal screening to cellular and molecular screening model, from the traditional drug screening technology to high efficient and throughput screening. This paper will summarize the technology of drug screening in the therapy of type 2 diabetes mellitus.
