[Progress of the application research of acupuncture anesthesia in thyroid surgery].

This article reviews the clinical studies on the application of acupuncture anesthesia in thyroid surgery over the past 5 years. Starting from the principle of acupuncture anesthesia, the authors explore the application of acupuncture anesthesia in thyroid surgery, its advantages and development. The summarized findings showed that acupuncture anesthesia has a better analgesic effect in thyroid surgery, which can reduce the occurrence of stress reactions, adverse reactions and complications, and is conducive to postoperative recovery. However, there is a lack of standardized operations. In the future, standardized operations can make acupuncture anesthesia more widely used and popularized.

An eight-coordinate ytterbium complex with a hexagonal bipyramid geometry exhibiting field-induced single-ion magnet behaviour.

Two isostructural eight-coordinate complexes Ln(NO3)3(tBu3PO)2 (Ln = Yb, 1-Yb; Tb, 2-Tb) were synthesized and structurally analyzed by single-crystal X-ray crystallography. Both complexes feature a nearly perfect hexagonal bipyramidal LnO8 geometry with ideal D3h symmetry. Magnetic measurements show field-induced slow magnetic relaxation for 1-Yb, thereby presenting the first report of YbIII-based SIMs with a hexagonal bipyramidal coordinate geometry. In contrast, 2-Tb shows no significant out-of-phase (χ''M) ac signals.

Serum autoantibodies against a panel of 15 tumor-associated antigens in the detection of ovarian cancer.

In this study, enzyme-linked immunosorbent assay has been used to examine the frequencies of serum autoantibodies against two candidate tumor-associated antigens intensively selected from the Human Protein Atlas database, in combination with 13 tumor-associated antigens available from our lab in sera from 44 OC patients and 50 normal healthy controls. Conventional evaluation (mean + 3SD as the cutoff value to determine a positive reactivity), receiver operating characteristic curve analyses, and classification tree analysis were further used to evaluate the diagnostic performance of autoantibodies against these tumor-associated antigens (anti-tumor-associated antigens) in ovarian cancer. For single anti-tumor-associated antigen, when the cutoff values were set as mean + 3SD of normal healthy controls, NPM1, MDM2, PLAT, p53, and c-Myc could achieve sensitivity higher than 20% at 98% specificity. Combinational utilization of autoantibodies against MDM2, PLAT, NPM1, 14-3-3 Zeta, p53, and RalA achieved the optimal diagnostic performance with 72.7% sensitivity at 96% specificity. Receiver operating characteristic curve analysis showed that the area under the receiver operating characteristic curves of autoantibodies against c-Myc, NPM1, MDM2, p16, p53, and 14-3-3 Zeta were greater than 0.80. This indicated that these tumor-associated antigens held high potential to serve as diagnostic biomarkers in ovarian cancer detection. Decision tree analysis indicated that anti-c-Myc held high potential in the detection of ovarian cancer. Further studies are warranted to validate the diagnostic performance of these anti-tumor-associated antigens with high area under the receiver operating characteristic curve, including autoantibodies against c-Myc, MDM2, PLAT, NPM1, 14-3-3 Zeta, p53, and RalA.

The effect of quercetin nanoparticle on cervical cancer progression by inducing apoptosis, autophagy and anti-proliferation via JAK2 suppression.

Cervical cancer is a cause of cancer death, making it as the one of the most common cause for death among women globally. Though many studies before have explored a lot for cervical cancer prevention and treatment, there are still a lot far from to know based on the molecular mechanisms. Janus kinase 2 (JAK2) has been reported to play an essential role in the progression of apoptosis, autophagy and proliferation for cells. We loaded gold-quercetin into poly (dl-lactide-co-glycolide) nanoparticles to cervical cancer cells due to the propertities of quercetin in ameliorating cellular processes and the easier absorbance of nanoparticles. Here, in our study, quercetin nanoparticles (NQ) were administrated to cells to investigate the underlying mechanism by which the cervical cancer was regulated. First, JAK2-inhibited carvical cancer cell lines were involved for our experiments in vitro and in vivo. Western blotting, quantitative RT-PCR (qRT-PCR), ELISA, Immunohistochemistry, and flow-cytometric analysis were used to determine the key signaling pathway regulated by JAK2 for cervical cancer progression. And the role of quercetin nanoparticles was determined during the process. Data here indicated that JAK2, indeed, expressed highly in cancer cell lines compared to the normal cervical cells. And apoptosis and autophagy were found in JAK2-inhibited cancer cells through activating Caspase-3, and suppressing Cyclin-D1 and mTOR regulated by Signal Transducer and Activator of Transcription (STAT) 3/5 and phosphatidylinositide 3-kinase/protein kinases (PI3K/AKT) signaling pathway. The cervical cancer cells proliferation was inhibited. Further, tumor size and weight were reduced by inhibition of JAK2 in vivo experiments. Notably, administration with quercetin nanoparticles displayed similar role with JAK2 suppression, which could inhibit cervical cancer cells proliferation, invasion and migration. In addition, autophogy and apoptosis were induced, promoting cervical cancer cell death. To our knowledge, it was the first time to evaluate the role of quercetin nanoparticles in improving cervical cancer from apoptosis, autophagy and proliferation, which could be a potential target for future therapeutic approach clinically.

Autoantibody response to Sui1 and its tissue-specific expression in hepatocellular carcinoma.

To investigate the immunogenicity of Homo sapiens putative translation initiation factor (Sui1) in hepatocellular carcinoma (HCC), enzyme-linked immunosorbent assay (ELISA) and Western blot were utilized to assess autoantibody responses to Sui1 in sera from HCC patients and healthy individuals. Indirect immunofluorescence (IIF) assay with cancer cells and immunohistochemistry (IHC) study with tissue array slides were performed to examine Sui1 expression profile in cancer cells and tissues. The data confirmed that the frequency of autoantibody to Sui1 in sera of HCC patients was 15.5 % (16/103), which was remarkably higher than that in sera of liver cirrhosis (LC) patients (3.3 %, 1/30), chronic hepatitis (CH) patients (0 %, 0/29), and normal human serum (NHS) (0 %, 0/82) (p < 0.01). IHC study showed that the Sui1 expression in HCC tissues was 26.7 % (16/60). The expression of Sui1 had the trend of increasing along with the cancer grades but no statistical significance (p > 0.05). In immunodiagnosis of HCC, the sensitivity and specificity of the anti-Sui1 antibody were 15.5 and 99.3 %, respectively. If both anti-Sui1 and alpha fetal protein (AFP) were simultaneously utilized as detective markers, 66.7 % (30/45) of HCC patients could be correctly distinguished. The results suggested that anti-Sui1 could be utilized as a supplementary serological marker for the detection of HCC and Sui1 might be associated to HCC carcinogenesis.

[Discussion on acupuncture for cerebral apoplexy from the view of rehabilitation].

Abundant experiences have already been accumulated in treatment of stroke with acupuncture. Development of rehabilitation theory also brings unprecedented opportunity and challenge to acupuncture. Combined with the modern rehabilitation theory and practice, it is very helpful to deepen the understanding on treatment of acupuncture for cerebral apoplexy and enhance the therapeutic effect in clinic by studying the mechanism of acupuncture treatment, opportunity of intervention, selection of acupoints, needling manipulations and quantity of stimulations etc. Through analysis on the necessity and the way of combination of acupuncture and modern rehabilitation, it is concluded that rehabilitation evaluation, rehabilitation phases and obstacle analysis should be taken as references by acupuncturists to reinforce the therapeutic effect and creditability of acupuncture treatment on cerebral apoplexy.

Exchange coupling and magnetic anisotropy in a family of bipyrimidyl radical-bridged dilanthanide complexes: density functional theory and ab initio calculations.

The origin of the magnetic anisotropy energy barriers in a series of bpym(-) (bpym = 2,2'-bipyrimidine) radical-bridged dilanthanide complexes [(Cp*2Ln)2(µ-bpym)](+) [Cp* = pentamethylcyclopentadienyl; Ln = Gd(III) (1), Tb(III) (2), Dy(III) (3), Ho(III) (4), Er(III) (5)] has been explored using density functional theory (DFT) and ab initio methods. DFT calculations show that the exchange coupling between the two lanthanide ions for each complex is very weak, but the antiferromagnetic Ln-bpym(-) couplings are strong. Ab initio calculations show that the effective energy barrier of 2 or 3 mainly comes from the contribution of a single Tb(III) or Dy(III) fragment, which is only about one third of a single Ln energy barrier. For 4 or 5, however, both of the two Ho(III) or Er(III) fragments contribute to the total energy barrier. Thus, it is insufficient to only increase the magnetic anisotropy energy barrier of a single Ln ion, while enhancing the Ln-bpym(-) couplings is also very important.

Magneto-structural correlations in a family of Fe(II)Re(IV)(CN)2 single-chain magnets: density functional theory and ab initio calculations.

Until now, the expressions of the anisotropic energy barriers Δξ and ΔA, using the uniaxial magnetic anisotropy D, the intrachain coupling strength J, and the high-spin ground state S for single-chain magnets (SCMs) in the intermediate region between the Ising and the Heisenberg limits, were unknown. To explore this relationship, we used density functional theory and ab initio methods to obtain expressions of Δξ and ΔA in terms of D, J, and S of six R4Fe(II)-Re(IV)Cl4(CN)2 (R = diethylformamide (1), dibutylformamide (2), dimethylformamide (3), dimethylbutyramide (4), dimethylpropionamide (5), and diethylacetamide (6)) SCMs in the intermediate region. The ΔA value for compounds 1-3 was very similar to the magnetic anisotropic energy of a single Fe(II), while the value of Δξ was predicted using the exchange interaction of Fe(II) with the neighboring Re(IV), which could be expressed as 2JSReSFe. Similar to compounds 1-3, the anisotropy energy barrier ΔA of compounds 4 and 5 was also equal to (Di - Ei)SFe(2), but the correlation energy Δξ was closely equal to 2JSReSFe(cos 98.4 - cos 180) due to the reversal of the spins on the opposite Fe(II). For compound 6, one unit cell of Re(IV)Fe(II) was regarded as a domain wall since it had two different Re(IV)-Fe(II) couplings. Thus, the Δξ of compound 6 was expressed as 4J″SRe1Fe1SRe2Fe2, where J″ was the coupling constant of the neighboring unit cells of Re1Fe1 and Re2Fe2, and ΔA was equal to the anisotropic energy barrier of one domain wall given by DRe1Fe1(S(2)Re1Fe1 - 1/4).

Understanding the magnetic anisotropy in a family of N2(3-) radical-bridged lanthanide complexes: density functional theory and ab initio calculations.

Density functional theory (DFT) and ab initio methods were used to investigate the influence of both intramolecular exchange coupling and single-ion anisotropy on the relaxation barriers of a series of N2(3-) radical-bridged lanthanide complexes [{[(Me3Si)2N]2(THF)Ln}2(µ-η(2):η(2)-N2)](-) (Ln = Gd(III) (1), Tb(III) (2), Dy(III) (3), Ho(III) (4), and Er(III) (5)) reported by Long and co-workers. DFT calculations show that the exchange coupling between the lanthanide ions is very weak, but the Ln-N2(3-) coupling is strong for each complex. Moreover, the exchange couplings of Ln-N2(3-) are antiferromagnetic for Ln = Gd(III), Tb(III), Dy(III), and Ho(III) but ferromagnetic for Er(III) for the nearly orthogonal magnetic orbitals on Er(III) and N2(3-). Ab initio calculations show that both of the large magnetic anisotropy of single Tb fragment and the strong Tb-N2(3-) antiferromagnetic couplings lead to the largest energy barrier of complex 2. Although the energy barrier of a single Er fragment is the second largest, the relaxation barrier of complex 5 is only 36.0 cm(-1) due to the weak Er(III)-N2(3-) coupling. This study suggests that both intramolecular exchange coupling and single-ion anisotropy contribute greatly to the full relaxation barrier of lanthanide-based single-molecule magnets (SMMs), which expands the understanding of SMMs using only the giant-spin model.

[Clinical observation on idiopathic sudden hearing loss treated by warming-promoting needling technique].

OBJECTIVE: To explore the effective therapeutic method in the treatment of idiopathic sudden hearing loss (ISHL). METHODS: One hundred and eighty-eight cases of ISHL were randomized into a warming-promoting needling group (74 cases), a conventional acupuncture group (56 cases) and a medication group (58 cases). In the conventional acupuncture group, the conventional needling technique was applied to Baihui (GV 20), Fengchi (GB 20), Yifeng (TE 17), Tinggong (SI 19), Touqiaoyin (GB 11) and Zhigou (TE 6) on the affected side. The treatment was given 5 times each week. Totally, the treatment of 6 weeks was required. In the warming-promoting needling group, on the basic treatment as the conventional acupuncture group, the warming-promoting needling technique was applied to Fengchi (GB 20). In the medication group, the intravenous drop with salvia injectio and mecobalamin was prescribed, once per day, for 10 days totally. Meanwhile, Erlong Zuoci Wan was prescribed for oral administration, 8 pills each time, three times a day for 30 days continuously. RESULTS: All of the three therapeutic methods achieved the effect on ISHL. The total effective rate was 89.2% (66/74) in the warming-promoting needling group, which was better than 62.5% (35/56) in the conventional acupuncture group and 53.4% (31/58) in the medication group (both P<0.01). CONCLUSION: The warming-promoting needling techinque achieves the significant efficacy on ISHL. The hearing improvement is superior to that treated with either the conventional needling technique or medication.

Exploring the sources of the magnetic anisotropy in a family of cyanide-bridged Ni9Mo6 and Ni9W6 systems: a density functional theory study.

A density functional theory (DFT) study of the magnetic coupling interactions and magnetic anisotropy in a family of experimentally synthesized Ni(9)Mo(V) and Ni(9)W(V) systems is presented. Our calculations show that for all of our selected Ni(9)M(6) systems, the intramolecular magnetic coupling interactions are ferromagnetic, and the ground-state spins are 12. All of the D values of Ni(9)W(6) systems come mainly from the contribution of the D(i) of W(6)(CN)(48)Ni extracted from Ni(9)W(6), and the influence of the eight surrounding Ni including the ligands on their magnetic anisotropy is very small. Although the surrounding Ni bounded by different ligands have a small influence on all D values for our selected complexes, they decide on the core structures of W(6)(CN)(48)Ni, which dominate their magnetic anisotropy. Thus, to obtain a Ni(9)W(6) system having a large negative D, we can use different ligands bound to Ni to obtain a good core structure of W(6)(CN)(48)Ni with a large negative D value. All D values of Ni(9)Mo(6) systems also come mainly from the contribution of D(i) of the Mo(6)(CN)(48)Ni, which is positive or negative but very small; most of these systems do not behave as single-molecule magnets.

On the origin of magnetic anisotropy in cyanide-bridged Co(9)M(6) (M = Mo(V) or W(V)) systems: a DFT study.

Our calculations show that the substitution of metal ions using Co(II) is not the main reason that Co(II)(9)M(6) (M = Mo(V) or W(V)) exhibits single-molecule-magnet behavior, whereas the unsymmetrical distribution of three CH(3)OH ligands surrounding each Co(II) is the main one.

Magnetic anisotropy in a family of experimentally synthesized and theoretically modeled M'6M8(CN24) systems.

A theoretical density functional study of the magnetic coupling interactions and magnetic anisotropy in a family of experimentally synthesized and theoretically modeled M'6M8(CN24) (M'=Cu(II), Ni(II) or Co(II); M=Fe(III) or Cr(III)) systems is presented. The calculations show that the interactions in the selected M'6M8(CN24) are all ferromagnetic and the near cubic symmetry of Cu6Fe8 is the origin of its negative magnetic anisotropy parameter D.

Theoretical study of magnetostructural correlations in a family of triangular manganese(III) complexes.

A theoretical density functional study of the magnetostructural correlations in a family of triangular [Mn(3)O](7+) systems is presented. Our calculations show that to obtain a good [Mn(3)O](7+) system with strong Mn-Mn ferromagnetic interactions and a large negative D value, we can first decrease tau formed by the two planes of Mn(1)NO(2-) and Mn(2)OO(2-) through changing the orientations of the terminal ligands involving mu-NO exchange pathways (this operation will weaken the Mn-Mn ferromagnetic interactions) to obtain a large negative D value, and then increase tau through distorting Mn-N-O-Mn angles and/or enlarge d (the deviation of the mu(3)-O(2-) ion from the [Mn(III)(3)] plane) to enhance the Mn-Mn ferromagnetic interactions.

Theoretical study of the exchange coupling substituting Mo(V) with W(V) in four cyano-bridged M'9M6 (M' = Mn(II) or Ni(II); M = Mo(V) or W(V)) systems.

Magnetic properties of four high-spin molecules [M'{M'(MeOH)3}8(mu-CN)30{M(CN)3}6] (M' = Mn(II) and M = Mo(V) for A; M' = Mn(II) and M = W(V) for B; M' = Ni(II) and M = Mov for C; M' = Ni" and M = W(V) for D) have been investigated using hybrid density functional theory (DFT) B3LYP with LANL2DZ basis set. Our calculations suggest that models [M'(mu-CN)6{M(CN)7}6](16-) (M' = Mn(II) and M = Mo(V) for A6; M' = Mn" and M = W(V) for B6; M' = Ni(II) and M = Mo(V) for C6; M' = Ni(II) and M = W(V) for D6) and [M'(MeOH)3(mu-CN)3{M(CN)7}3](7-) (M' = Mn(II) and M = Mo(V) for A3; M' = Mn(II) and M = W(V) for B3; M' = Ni(II) and M = Mo(V) for C3; M' = Ni(II) and M = W(V) for D3) are good candidates to model the complete molecular structures of A, B, C and D. For all complexes and models, the antiferromagnetic or ferromagnetic exchange interactions through equatorial cyanides are all stronger than those through apical cyanides. In Mn9M6 (A and B) systems, substituting Mo with W always enhances the antiferromagnetic interactions for the apical and equatorial cyanides and the different Cbrid-Nbrid-Mn angles, but the behavior is more complicated for Ni9M6 (C and D). Moreover, the calculated delta(xy) values of a series of binuclear models according to Kahn-Briat model were used to confirm our conclusions.

Theoretical study of the relationship between the nearest-neighbor exchange coupling interactions and the number of peripheral complexes in the cyano-bridged CrMn6(CN)6 and CrMn2(CN)2 clusters.

A theoretical density functional study of the relationship between the nearest-neighbor constants and the number of peripheral complexes in the cyano-bridged [Cr[CNMn(salen)(H2O)]6]3+ and [(5-Brsalen)2(H2O)2Mn2Cr(CN)6] clusters is presented. Two approaches show that the antiferromagnetic coupling interactions between nearest neighbors decrease with the increase of the number of peripheral complexes, although the second approach provides better results using several exchange-correlation functionals. The first approach consisted of evaluating the exchange coupling constant J(ij) between two paramagnetic metal centers i and j in the hexanuclear molecule by calculating the energy differences between the highest and broken-symmetry spin states of a model molecule in which metal atoms except for i and j are substituted by diamagnetic Zn(II) cations, while the second consisted of calculating the different spin-state energies of hexanuclear complexes and using the Heisenberg Hamiltonian to obtain the exchange coupling constants between different metal centers. Moreover, Kahn's qualitative theory succeeded in being applied to interpret the trend.