RESUMO
Duck liver is an important by-product of duck food. In this study, we investigated the effects of glucose, fructose, and xylose on the antioxidant properties of glycosylated products of duck liver protein and their protective effects on HepG2 cells. The results show that the glycosylation products of the three duck liver proteins (DLP-G, DLP-F, and DLP-X) all exhibit strong antioxidant activity; among three groups, DLP-X shows the strongest ability to scavenge DPPH, ·OH free radicals, and ABTS+ free radicals. The glycosylated products of duck liver protein are not toxic to HepG2 cells and significantly increase the activity of antioxidant enzymes such as SOD, CAT, and GSH-Px in HepG2 cells at the concentration of 2.0 g/L, reducing oxidative stress damage of cells (p < 0.05). DLP-X has a better effect in reducing oxidative damage and increasing cellular activity in HepG2 cells than DLP-G and DLP-F (p < 0.05). In this study, the duck liver protein glycosylated products by glucose, fructose, and xylose were named as DLP-G, DLP-F, and DLP-X, respectively.
RESUMO
A shipment of Fults alkaligrass seed (Puccinellia distans) grown in Washington state containing bunted florets was intercepted by quarantine officials at China's Tianjin Entry-Exit Quarantine and Inspection Bureau. The bunted florets were filled with irregularly shaped, reticulately ornamented teliospores that germinated in a manner characteristic of systemically infecting Tilletia spp. on grass hosts in subfamily Pooideae. Based on morphological characters and a multigene phylogenetic analysis of the ITS region rDNA, eukaryotic translation elongation factor 1 alpha and a region of the second largest subunit of RNA polymerase II including a putative intein, the Puccinellia bunt is genetically distinct from known species of Tilletia and is proposed as a new species, T. puccinelliae.
Assuntos
Basidiomycota/classificação , Doenças das Plantas/microbiologia , Poaceae/microbiologia , Sementes/microbiologia , Esporos Fúngicos/fisiologia , Basidiomycota/genética , Basidiomycota/isolamento & purificação , Basidiomycota/fisiologia , China , DNA Fúngico/análise , DNA Espaçador Ribossômico/análise , DNA Espaçador Ribossômico/genética , Técnicas de Tipagem Micológica , Fator 1 de Elongação de Peptídeos/genética , Filogenia , RNA Polimerase II/genética , Análise de Sequência de DNA , Especificidade da Espécie , Esporos Fúngicos/genética , WashingtonRESUMO
A bunt fungus, exhibiting a spore germination pattern unique to known reticulate-spored species of Tilletia was found infecting plants in seed production fields of Festuca rubra ssp. rubra (red fescue) and F. rubra ssp. fallax (Chewing's fescue) in Oregon, and in seed lots of Lolium perenne (perennial ryegrass) from Australia and Germany. Teliospores germinated to form 20-40 uninucleate, non-conjugating basidiospores, and colonies derived from single basidiospores produced teliospores in culture. In inoculation studies using single basidiospore colonies, perennial ryegrass and L. perenne ssp. multiflorum (Italian or annual ryegrass) were infected. A phylogenetic analysis, based on ITS region rDNA, eukaryotic translation elongation factor 1 alpha, and the second largest subunit of RNA polymerase II demonstrated that the fescue and ryegrass bunts are conspecific, and distinct from known species of Tilletia.
