RESUMO
Cyclin D1b, a splice variant of the cell cycle regulator cyclin D1, holds oncogenic functions in human cancer. However, the mechanisms underlying cyclin D1b function remain poorly understood. Here we introduced wild-type cyclin D1a or cyclin D1b variant into non-metastatic MCF-7 cells. Our results show that ectopic expression of cyclin D1b promotes invasiveness of the cancer cells in a cyclin D1a independent manner. Specifically, cyclin D1b is found to modulate the expression of αvß3, which characterizes the metastatic phenotype, and enhance tumor cell invasive potential in cooperating with HoxD3. Notably, cyclin D1b promotes αvß3-mediated adhesion and invasive migration, which are associated with invasive potential of breast cancer cells. Further exploration indicates that cyclin D1b makes breast cancer cells more sensitive to toll-like receptor 4 ligand released from damaged tumor cells. These findings reveal a role of cyclin D1b as a possible mediator of αvß3 transcription to promote tumor metastasis.
Assuntos
Neoplasias da Mama/metabolismo , Adesão Celular , Ciclina D1/metabolismo , Integrina alfaVbeta3/metabolismo , Integrina beta3/metabolismo , Animais , Neoplasias da Mama/genética , Neoplasias da Mama/patologia , Movimento Celular , Ciclina D1/genética , Feminino , Proteínas de Homeodomínio/metabolismo , Humanos , Integrina alfaVbeta3/genética , Integrina beta3/genética , Ligantes , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/secundário , Metástase Linfática , Células MCF-7 , Camundongos Endogâmicos BALB C , Camundongos Nus , Invasividade Neoplásica , Fenótipo , Isoformas de Proteínas , Fatores de Tempo , Receptor 4 Toll-Like/metabolismo , Fatores de Transcrição , TransfecçãoRESUMO
NAC family is a class of transcription factors that have been typically found in plant with various functions. This type of genes plays a key regulatory role in secondary plant growth, cell division, senescence, especially in the hormone and signaling pathways. ANAC092 was reported to function in relation to lateral root development and senescence. The promoter and in situ hybridization analyses showed that ANAC092 was expressed temporally in the process of anther development. The gene was expressed in tapetum at stages 8-11 of anther development and reached the highest amount at stages 9-10, which was overlapped with the expression period of AMS (Aborted microspores). In this study, we constructed ANAC092 over-expression system, and identified homozygous transgenic lines. Compared with the wild type, the number of pollen grains in the transgenic line was decreased significantly, but the length of pollen grain was increased. qRT-PCR analysis showed that the expression of genes related to pollen development, e.g., SPL, EMS1, DYT1, and AMS was in-creased in the over-expression plants. Bioinformatics results showed that ANAC092 promoter sequence possessed seven AMS binding sites. All the results showed that ANAC092 is possibly located in the downstream of AMS and plays an im-portant role in the process of pollen development.
Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Flores/crescimento & desenvolvimento , Regulação da Expressão Gênica de Plantas , Proteínas Repressoras/metabolismo , Fatores de Transcrição/metabolismo , Arabidopsis/genética , Arabidopsis/crescimento & desenvolvimento , Proteínas de Arabidopsis/genética , Flores/genética , Flores/metabolismo , Pólen/genética , Pólen/crescimento & desenvolvimento , Pólen/metabolismo , Proteínas Repressoras/genética , Fatores de Transcrição/genéticaRESUMO
OBJECTIVE: To investigate magnetic resonance imaging (MRI) characteristics of ovarian carcinosarcoma and the diagnostic value of MRI. METHODS: The MRI features of ovarian carcinosarcoma and clinical data of 5 patients with ovarian carcinosarcoma were reviewed. All the lesions were confirmed by surgery and pathological examination. RESULTS: MRI of ovarian carcinosarcoma in the 5 cases all showed large tumor mass and heterogeneous high-intensity on T2-weighted images and low-intensity on T1-weighted images, with laminar or stripe-like enhancement. Hemorrhage and necrosis were also displayed in some lesions. In two cases, the tumors invaded the greater omentum, sigmoid colon and the body of the uterus, with regional lymph node involvement. Pelvic effusion was observed in all the cases with pelvic hematocele in 1 case. CONCLUSION: MRI is useful in the detection and staging of ovarian carcinosarcoma.
