In situ phytoextraction of Mn and NH4+-N from aqueous electrolytic manganese residue solution by Pistia stratiotes: Effects of Fe/Co presence and rhizospheric microbe synergistic involvement.

The electrolytic manganese industry produces a large amount of electrolytic manganese residue (EMR). Soluble Mn, NH4+-N, and other pollutants may be released from the open-air stacked EMR and transported to the environment along with rainfall or surface runoff. Aqueous EMR solution (AES) generally contains various elements required for plant growth, and phytoremediation can be applied to remove these pollutants from AES. Since the contents of Fe and Co vary greatly in AES depending on the ore sources as well as the pre-treatment processes, the presence of bioavailable Fe and Co at different levels may affect plant growth, the rhizosphere microbes, and pollutant removal. The present study investigated the in-situ removal of Mn(II) and NH4+-N from AES solution using free floating aquatic plant Pistia stratiotes, focusing especially on the effects of Fe/Co presence and rhizospheric microbe synergistic involvement on contaminant removal. The results showed that 69.08% of Mn and 94.99% of NH4+-N were removed by P. stratiotes in 24 d. Both the presence of Fe(II) and Co(II) facilitated the Mn(II) immobilization and increased Mn(II) removal by 19-31% due to the enhanced peroxidase activity and the increased Mn accumulating in roots The complete removal of Mn from AES was found in the presence of Fe(II) at 2 mg L-1 or Co(II) at 0.5 mg L-1 and more than 51% accumulated Mn in the roots was stored in the vacuole and cytoplasm. BioMnOx was found on the surface of the roots, revealing that rhizofiltration, rhizospheric plaque/biofilm formation, and Mn biogeochemical cycle exert synergic effects on Mn(II) immobilization. The findings of the present study demonstrate the feasibility of using P. stratiotes in the treatment of aqueous EMR solutions and the presence of an appropriate amount of bio-available Fe and Co can promote the removal of Mn(II) and NH4+-N.

Diesel degradation capability and environmental robustness of strain Pseudomonas aeruginosa WS02.

Petroleum hydrocarbon (PHC) degrading bacteria have been frequently discovered. However, in practical application, a single species of PHC degrading bacterium with weak competitiveness may face environmental pressure and competitive exclusion due to the interspecific competition between petroleum-degrading bacteria as well as indigenous microbiota in soil, leading to a reduced efficacy or even malfunction. In this study, the diesel degradation ability and environmental robustness of an endophytic strain Pseudomonas aeruginosa WS02, were investigated. The results show that the cell membrane surface of WS02 was highly hydrophobic, and the strain secreted glycolipid surfactants. Genetic analysis results revealed that WS02 contained multiple metabolic systems and PHC degradation-related genes, indicating that this strain theoretically possesses the capability of oxidizing both alkanes and aromatic hydrocarbons. Gene annotation also showed many targets which coded for heavy metal resistant and metal transporter proteins. The gene annotation-based inference was confirmed by the experimental results: GC-MS analysis revealed that short chain PHCs (C10-C14) were completely degraded, and the degradation of PHCs ranging from C15-C22 were above 90% after 14 d in diesel-exposed culture; Heavy metal (Mn2+, Pb2+ and Zn2+) exposure was found to affect the growth of WS02 to some extent, but not its ability to degrade diesel, and the degradation efficiency was still maintained at 39-59%. WS02 also showed a environmental robustness along with PHC-degradation performance in the co-culture system with other bacterial strains as well as in the co-cultured system with the indigenous microbiota in soil fluid extracted from a PHC-contaminated site. It can be concluded that the broad-spectrum diesel degradation efficacy and great environmental robustness give P. aeruginosa WS02 great potential for application in the remediation of PHC-contaminated soil.

A manganese-oxidizing bacterium-Enterobacter hormaechei strain DS02Eh01: Capabilities of Mn(II) immobilization, plant growth promotion and biofilm formation.

While biogenic Mn oxides (BioMnOx) generated by Mn(II)-oxidizing bacteria (MOB) have attracted increasing attention, a MOB strain isolated from Mn-polluted sediments was identified and assigned as Enterobacter hormaechei DS02Eh01. Its Mn(II) immobilization activity, plant growth-promoting traits, and biofilm formation capability were investigated. The results showed that strain DS02Eh01 was found to be able to tolerate Mn(II) up to 122 mM. The strain immobilized Mn(II) in aquatic media mainly through extracellular adsorption, bio-oxidation and pH-induced precipitation as well as manganese oxidation. DS02Eh01-derived BioMnOx are negatively charged and have a larger specific surface area (86.70 m2/g) compared to the previously reported BioMnOx. The strain can immobilize Mn(II) at extreme levels, for instance, when it was exposed to 20 mM Mn(II), about 59% of Mn(II) were found immobilized and 17% of Mn(II) were converted to MnOx. The SEM and TEM observation revealed that the DS02Eh01-derived BioMnOx were aggregates doped with granules and microbial pellets. The precipitated Mn(II) and the Mn(III)/Mn(IV) oxides co-existed in BioMnOx, in which Mn(II) and Mn(IV) were found dominant with Mn(II) accounting for 49.6% and Mn(IV) accounting for 41.3%. DS02Eh01 possesses plant growth-promoting traits and biofilm formation capacity even under Mn(II) exposure. Mn(II) exposure at 5 mM was found to stimulate strain DS02Eh01 to form biofilms, from which, the extracted EPS was mainly composed of aromatic proteins. This study reveals that E. hormaechei strain DS02Eh01 possesses the potential in environmental ecoremediation via coupling processes of macrophytes extraction, biochemical immobilization and biosorption.

Coupling suspect and non-target analytical methods for screening organic contaminants of concern in agricultural & urban impacted waters: Optimization and application.

Long-term exposure of contaminants to emerging concern (CECs) may pose risks to human health and ecosystems, even at low concentrations. Rivers impacted by both agricultural and urban activities experience distinctive environmental pressures due to receiving wastewaters that contain complex organics and their transformation products (TPs). In this study, we developed a regional database composed of 1200 CECs of high concern in Guangxi (South China). Further, we optimized a comprehensive analytical method for simultaneously screening for CECs and their TPs. The optimized screening method was applied to surface waters sampled from 10 different cross sections of a river that is impacted by both agricultural and urban activities. The best results of method optimization were achieved when the screening detection limit (SDL) ranged from 0.05 to 2 ng L-1, and over 90% of the analytes had acceptable recovery rates ranging between 64.7% and 95.6% (RSD < 11%). Of the 1200 CECs contained in the regional database, 168 were detected in at least one sampling site of the studied river via suspect screening, and among them, 36 contaminants were found at all sampling sites. Also, 58 additional contaminants and 39 TPs were tentatively identified via non-target screening, among which 4 TPs were reported for the first time in the aquatic environment. Triazine herbicides and their TPs were identified at most of the sampling sites, with ametryn and atrazine posing relatively high risks in the river ecosystems. Furthermore, 31 known analytes were selected as standards in order to confirm the combined screening method; one false positive occurred in the non-target screening method. According to these results, the suspect screening strategy provides valuable confirmation for the identification of a wide range of CECs in water, while non-target screening can provide a reference for researchers and supplement the regional database, particularly in the study of TPs.

A novel manganese oxidizing bacterium-Aeromonas hydrophila strain DS02: Mn(II) oxidization and biogenic Mn oxides generation.

The extensive applications of biogenic manganese oxides (BioMnOx) generated by manganese oxidizing bacteria (MOB) have attracted considerable attentions. In this study, we report on a novel MOB that has been isolated from sediments and identified as Aeromonas hydrophila strain DS02. The Mn(II) oxidation activity of strain DS02 under Mn(II) stress and the application of the associated BioMnOx products were investigated. Nearly 90.0% (495 mg L-1) of the soluble Mn(II) were removed and 45.6% (240 mg L-1) was converted to Mn(III/IV). Fitting the XPS data showed that Mn(IV)-oxide is the major component (82.0%) of the flake-shaped BioMnOx, corresponding to an average Mn oxidation number of 3.71. When the BioMnOx were coupled with the PMS activation, a 99.5% catalytic degradation of 2,4-dimethylaniline was observed after 80 min, revealing a high degradation efficiency.