Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 5 de 5
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
J Trace Elem Med Biol ; 84: 127449, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38640746

RESUMO

BACKGROUND: The effects of prenatal element exposure on mothers and fetuses have generated concern. Profiles of trace and toxic elements in biological material are urgently desired, especially for women who reside near e-waste recycling facilities. The aim of this study was to investigate elements concentrations in placenta, cord blood, and maternal blood of women and to evaluate the influencing factors. METHODS: A group of 48 women from an e-waste recycling site and a group of 31 women from a non-e-waste recycling site were recruited. Basic characteristics were collected by questionnaire and the concentrations of 17 elements in placenta, cord blood, and maternal blood samples were analyzed by inductively coupled plasma mass spectrometry (ICP-MS). Finally, the generalized linear model regression analysis (GLM) was used to test the association between element concentrations and possible factors. RESULTS: Compared to the control group, the exposed group had significantly elevated cadmium (Cd), zinc (Zn), nickel (Ni), and antimony (Sb) in placenta, and higher lead (Pb) in maternal blood and cord blood (P<0.05). Sb concentration in maternal blood was significantly lower than in the control group (P<0.05). GLM analysis showed that element concentrations were mainly associated with maternal age [chromium (Cr), iron (Fe), selenium (Se), cobalt (Co), mercury (Hg) in placenta, copper (Cu) in maternal blood], education (Se, Sb in placenta), family income (Cu in maternal blood and Ni in placenta), passive smoking [Cu and Zn in placenta, Pb in maternal blood], and e-waste contact history (Hg in cord blood, Cu, Zn, and Cd in maternal blood). CONCLUSIONS: Women in the e-waste recycling area had higher toxic element levels in the placenta and blood samples. More preventive measures were needed to reduce the risk of element exposure for mothers and fetuses in these areas.


Assuntos
Resíduo Eletrônico , Sangue Fetal , Placenta , Humanos , Feminino , Sangue Fetal/química , Sangue Fetal/metabolismo , Gravidez , Adulto , Placenta/metabolismo , Placenta/química , Reciclagem , Oligoelementos/sangue , Adulto Jovem
2.
Anal Chem ; 85(20): 9428-32, 2013 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-24069956

RESUMO

Highly sensitive and a multiplex assay of viruses and viral DNAs in complex biological samples is extremely important for clinical diagnosis and prognosis of pathogenic diseases as well as virology studies. We present an effective ICP-MS-based multiplex and ultrasensitive assay of viral DNAs with lanthanide-coded oligonucleotide hybridization and rolling circle amplification (RCA) strategies on biofunctional magnetic nanoparticles (MNPs), in which single-stranded capture DNA (ss-Cap-DNA)-functionalized MNPs (up to 1.65 × 10(4) ss-Cap-DNA per MNP) were used to recognize and enrich target DNAs, and single-stranded report DNA (ss-Rep-DNA-DOTA-Ln) coded by the lanthanide-DOTA complex hybridized with the targeted DNA for highly sensitive readout of HIV (28 amol), HAV (48 amol), and HBV (19 amol). When utilizing the RCA technique in association with the design and synthesis of a "bridge" DNA and a corresponding ss-Rep-DNA-DOTA-Ho, as low as 90 zmol HBV could be detected. Preliminary applications to the determination of the viral DNAs in 4T1 cell lysates and in serum confirmed the feasibility of this ICP-MS-based multiplex DNA assay for clinical use. One can expect that this element-coded ICP-MS-based multiplex and ultrasensitive DNA assay will play an ever more important role in the fields of bioanalysis and virology and in medical studies after further sophisticated modifications.


Assuntos
Técnicas Biossensoriais/métodos , Elementos da Série dos Lantanídeos/química , Espectrometria de Massas , Técnicas de Amplificação de Ácido Nucleico/métodos , Vírus/isolamento & purificação , DNA de Cadeia Simples/química , DNA Viral/análise , DNA Viral/química , DNA Viral/genética , Hibridização de Ácido Nucleico , Vírus/genética
3.
Anal Chem ; 84(21): 8946-51, 2012 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-23075036

RESUMO

We report the design and synthesis of a trifunctional probe for seeing and counting cancer cells using both fluorescence imaging (FI) and inductively coupled plasma mass spectrometry (ICPMS) for the first time. It consisted of a guiding cyclic RGD peptide unit to catch cancer cells via targeting the α(v)ß(3) integrin overexpressed on their surface, a 5-amino-fluorescein moiety for FI using confocal laser scanning microscopy (CLSM) as well as a 2-aminoethyl-monoamide-DOTA group for loading stable europium ion and subsequent ICPMS quantification of the cancer cells without the use of radioactive isotopes. In addition to FI, the LOD (3σ) of the α(v)ß(3) integrin was down to 69.2-309.4 amol per cell depending on the type of the α(v)ß(3)-positive cancer cells when using ICPMS and those of the cancer cell number reached 17-75. This probe developed enables us not only to see but also to count the α(v)ß(3)-positive cancer cells ultrasensitively, paving a new way for early diagnosis of cancer.


Assuntos
Contagem de Células/métodos , Integrina alfaVbeta3/metabolismo , Sondas Moleculares/metabolismo , Neoplasias/metabolismo , Neoplasias/patologia , Amidas/química , Linhagem Celular Tumoral , Desenho de Fármacos , Európio/química , Fluoresceína/química , Corantes Fluorescentes/química , Compostos Heterocíclicos com 1 Anel/química , Humanos , Sondas Moleculares/química , Oligopeptídeos/química
4.
Anal Chem ; 84(6): 2974-81, 2012 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-22380461

RESUMO

We reported novel Ag-TiO(2)- and ZrO(2)-based photocatalytic vapor generation (PCVG) systems as effective sample introduction techniques for further improving the sensitivity of the atomic spectrometric determination of selenium for the first time, in which the conduction band electron served as a "reductant" to reduce selenium species including Se(VI) and convert them directly into volatile H(2)Se, which was easily separated from the sample matrix and underwent more effectively subsequent atomization and/or ionization. These two PCVG systems helped us to overcome the problem encountered in the most conventional KBH(4)/OH(-)-H(+) system, in that Se(VI) was hardly converted into volatile selenium species without the aid of prereduction procedures. The limits of detection (LODs) (3σ) of the four most typical Se(IV), Se(VI), selenocystine ((SeCys)(2)), and selenomethionine (SeMet) species were, respectively, down to 1.2, 1.8, 7.4, and 0.9 ng mL(-1) in UV/Ag-TiO(2)-HCOOH, and 0.7, 1.0, 4.2, and 0.5 ng mL(-1) in UV/ZrO(2)-HCOOH with the relative standard deviations (RSDs) lower than 5.1% (n = 9 at 1 µg mL(-1)) when using atomic fluorescence spectrometry (AFS) under flow injection mode. They reached 10, 14, 18, and 8 pg mL(-1) in UV/Ag-TiO(2)-HCOOH, and 6, 7, 10, and 5 pg mL(-1) in UV/ZrO(2)-HCOOH with the RSDs lower than 4.4% (n = 9 at 10 ng mL(-1)) when using inductively coupled plasma mass spectrometry (ICPMS). After the two PCVG systems were validated using certified reference materials GBW(E)080395 and SELM-1, they were applied to determine the total Se in the selenium-enriched yeast sample and used as interfaces between high-performance liquid chromatography (HPLC) and AFS or ICPMS for selenium speciation in the water- and/or enzyme-extractable fractions of the selenium-enriched yeast.

5.
Angew Chem Int Ed Engl ; 51(14): 3358-63, 2012 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-22344943

RESUMO

Click and analyze: the titled probe was synthesized by conjugating a sulfonyl fluoride and azido unit using click chemistry to give SF-Eu, which can react specifically with serine (Ser) in the active site of serine protease (SP). Combination of the method with (153)Eu-isotope dilution ICP/MS enables absolute protein quantification of active SPs in biological samples using only one (153)Eu(NO(3))(3) isotopic standard.


Assuntos
Química Click , Európio/química , Espectrometria de Massas , Serina Proteases/análise , Domínio Catalítico , Cromatografia Líquida de Alta Pressão , Compostos Heterocíclicos com 1 Anel/química , Marcação por Isótopo , Ácidos Sulfínicos/química
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...