[Comparative Study on Protection Effects of CCl4-Induced Acute Liver Injury of Different Prepared Products from Gardenia jasminoides Fruits in Rats].

Objective: To provide the experimental evidence for the appropriate selection of the different prepared products from Gardenia jasminoides fruits by comparing their protection effects on carbon tetrachloride（ CCl4）-induced acute liver injury. Methods: The activities of ALT,AST,ADA,LDH,ALP and contents of PA,TP,TBIL,DBIL,TBA in serum,the activities of SOD and the content of MDA in liver tissue were measured in acute liver injury rats by carbon tetrachloride. Also the pathological changes of liver tissues were examined under microscope. Results: The biochemical indexes of AST,ALT,TBA,ADA,LDH and MDA were significantly improved in all groups of prepared products from Gardenia jasminoides fruits,but not SOD and ALP. The lesions of liver tissue had different degrees of reduction. Conclusion: The different prepared products from Gardenia jasminoides fruits had the effects of liver protection. The nut of Gardeniae Fructus was superior to the peel in enzyme decreasing and liver protection. The crude was superior to the stir-cooked in enzyme decreasing and liver protection.

[Study on chemical constituents of Gardenia jasminoides(III)].

OBJECTIVE: To investigate the chemical constituents of Gardenia jasminoides fruits. METHODS: Various column chromatography were used in the isolation and purification, and physiochemical constant determination and spectral analysis were adopted to determine the chemical structures. RESULTS: Twelve compounds were isolated from Gardenia jasminoides including jasminoside I (1), gardenoside (2), gardaloside (3), 3-hydroxy-urs-12-ene-11-ketone(4), 5, 4'-dihydroxyl-7, 3', 5'-trimethoxyflavone (5), 5, 7, 3', 4', 5'-pentamethoxyflavone(6), 3, 5, 6, 4'-tetrahydroxy-3', 5'-dimethoxyflavone (7), shikimic acid (8), 1, 2, 4-benzenetriol (9), 3, 4-dimethoxy-benzoic acid (10), dibutyl phthalate (11) and diisobutyl phthalate (12). CONCLUSION: Compounds 4 - 7 and 9 -10 were isolated from this plant for the first time.

[Study on the chemical components of Gardenia jasminoides (II)].

OBJECTIVE: To investigate the chemical components of Gardenia jasminoides. METHODS: Various column chromatography were used in the isolation and purification, and physiochemical constant determination and spectral analysis were adopted to idenitify the chemical structures. RESULTS: Ten compounds were isolated and identified as jasminoside A(1), epijasminoside A(2), 6-O-methylscandoside methyl ester (3), 6-O-methyldeacetylasperulosidic acid methyl ester (4), gardenoside (5), phenylmethol (6), 4-hydroxy-phenylmethol-O-beta-D-glucopyranosyl- (1-->6) -beta-D-glucopyranoside (7), 3,4-dihydroxy-phenylmethol-O-beta-D-glucopyranosyl-(1-6)-beta-D-glucopyranoside (8), 3-hydroxy4-methoxy-phenylmethol-O-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside (9), 3-hydroxy-4-methoxyphenylmethol-O-beta-D-glucopyranoside (10). CONCLUSION: Compounds 6 -10 are isolated from this plant for the first time.