An iterative approach to enhance the clinical learning experience in Macao nursing education.

OBJECTIVE: The aim of this study was to reduce or eliminate the stressors to enhance nursing students' clinical learning experience through implementation of an iterative approach that developed a personalised response to student stress. METHODS: A prospective cohort research design was applied to collect data from a sample of undergraduate nursing students across the four study years. An iterative approach was employed to improve students' learning experience and the Stressors in Nursing Students Scale-Chinese Version (SINS-CN) was used to measure student stress. Key problems encountered by students were identified, refined and the responding solutions were worked out and implemented among this group of students through their first year to fourth year. RESULTS: The overall SINS-CN mean score (2.17-2.82) of students was declined to a moderate level. CONCLUSIONS: Having implemented iterative approach to address factors that led to stressful environments encountered by the nursing students, the overall stress score and each sub-dimension score decreased significantly. Therefore, it is recommended that this approach could be adopted by other colleagues in the nursing arena around the world.

Combined magnetic porous molecularly imprinted polymers and deep eutectic solvents for efficient and selective extraction of aristolochic acid I and II from rat urine.

Aristolochia and related plants contain nephrotoxins and mutagens in the form of aristolochic acids (AAs). However, there is still lack of a fast and specific method for monitoring AAs in biological samples. Herein, we synthesized a hybrid magnetic mesoporous carbon-molecularly imprinted polymers (MMC@MIPs) as a novel magnetic solid-phase extraction (MSPE) adsorbent for selective recognition of aristolochic acid I and II from rat urine samples. The choline chloride/glycol-based deep eutectic solvent (DES) and indomethacin were used as the eluent and dummy template molecule accordingly. The morphology, structure property and surface groups of the prepared materials were investigated in sequence, and the optimum conditions of the MMC@MIPs-MSPE procedure were also optimized well. Results showed that the proposed method had a relatively satisfactory recovery (86.7-94.3%), with low standard deviation (<4.85%) and acceptable correlation coefficients (0.991-0.996). Overall, this work not only provides an inexpensive and eco-friendly method to fabricate MMC@MIPs, but also develops a highly promising approach for the detection of aristolochic acid I and II in biological samples.

Hybrid-type carbon microcoil-chitosan composite for selective extraction of aristolochic acid I from Aristolochiaceae medicinal plants.

Aristolochic acid I is a nephrotoxic compound widely existing in many kinds of traditional Chinese medicines, especially in Aristolochiaceae medicinal plants. In this study, chitosan modified carbon microcoils were designed and prepared for the selective separation of aristolochic acid I from medicinal herbs. Successful modification of carbon microcoils was confirmed by scanning electron microscopy, Fourier-transfer infrared spectroscopy, elemental analysis, X-ray photoelectron spectroscopy, and thermogravimetric analyses. The effects of adsorption conditions were investigated and it was determined that the adsorption of aristolochic acid I was controlled by pH. Adsorption isotherms, kinetics, and selectivity tests were performed to evaluate the adsorption capacity and selectivity of the modified carbon microcoils. The chitosan modified carbon microcoils exhibited excellent binding ability (77.72 mg g-1) and satisfactory selectivity. Finally, this material was used in solid phase extraction combined with HPLC to enrich and detect aristolochic acid I from medicinal plants. The detector response for aristolochic acid I was linear from 0.5 to 150 mg L-1, and the recoveries of aristolochic acid I ranged from 73.61 to 77.73% with the relative standard deviations of less than 5%. Thus, chitosan modified carbon microcoils were ideal adsorbents for the selective extraction of aristolochic acid I from Aristolochiaceae plants.

[IHA detection results of outpatients in schistosomiasis clinic, 2005-2014].

OBJECTIVE: To understand the changes of positive rates of IHA detections of outpatients in schistosomiasis clinic. RESULTS: The data of IHA detections of outpatients in schistosomiasis clinic in Hubei Provincial Center for Disease Control and Prevention were collected and analyzed statistically from 2005 to 2014. RESULTS: A total of 7 113 outpatients were detected by IHA test, and 547 of them were positives with a positive rate of 7.69%. The positive rate of IHA test was on an upward slope before 2008, and the rate reached 14.85% in 2008, which was significantly higher than that in 2005 (5.81%) (χ² = 47.40, P < 0.01), then it was on a declined stage after 2008, and the positive rate decreased to 3.76 in 2014, which was significantly lower than that in 2008 (χ² = 12.29, P < 0.01). The positive rate of outpatients in the 10 ~ < 30 years age group was higher than those in other age groups (all P < 0.012 5), and the male positives were more than the female ones. CONCLUSIONS: The schistosomiasis endemic situation has been significantly decreased in Hubei Province. The male and people in 10 ~ < 30 age group are the high risk groups, so the targeted health education should be strengthened.

ß-Cyclodextrin anchoring onto pericarpium granati-derived magnetic mesoporous carbon for selective capture of lopid in human serum and pharmaceutical wastewater samples.

Functionalized magnetic carbonaceous nanomaterials, which are important materials with many practical and research applications in biomedical, pharmaceutical and biological fields, have recently attracted much attention. In this study, a magnetic mesoporous carbon coated with ß-cyclodextrin (MMC@ß-CD) was synthesized for the first time from natural pericarpium granati (PG). The as-obtained MMC@ß-CD has high surface areas (203 m(2)g(-1)), large pore volumes (0.16 cm(3)g(-1)), relatively broad mesoporous sizes (6.8 nm) and a high saturation magnetization of 26.2 emu g(-1), which is sufficient for magnetic separation by an external magnetic field. The MMC@ß-CD was used as an innovative adsorbent for magnetic solid-phase extraction of lopid via host-guest interaction prior to spectrofluorometric analysis. The proposed method was successfully applied to analyze lopid in human serum and pharmaceutical wastewater samples with recoveries in the range of 85.0-103.5% for the spiked samples. Overall, this work not only provides an inexpensive and eco-friendly method to fabricate MMC@ß-CD (or MMC) from PG, but also develops a highly selective approach for capture of lopid in biological samples and environmental substances.

Hydrophilic Nitrogen and Sulfur Co-doped Molybdenum Carbide Nanosheets for Electrochemical Hydrogen Evolution.

Nitrogen and sulfur dual-doped Mo2 C nanosheets provide low operating potential (-86 mV for driving 10 mA cm(-2) of current density). Co-doping of N and S heteroatoms can improve the wetting property of the Mo2C electrocatalyst in aqueous solution and induce synergistic effects via σ-donation and π-back donation with hydronium cation.

[Discussion of indoor quality control method of MPAIA antibody test of Schistosoma japonicum].

OBJECTIVE: To explore the scientific and effective indoor quality control method to ensure the results of Magnetic Particle Antibody Immunoassay (MPAIA) antibody test of Schistosoma japonicum reliable. METHODS: The MPAIA antibody test indoor quality control work was performed with the combination of the crubbs method, Z-scores figure and Westgard multi-rules quality control method. RESULTS: The crubbs test entered the quality control condition state from the third times, after 20 times, the test switched to Z-scores conventional quality control, and the Westgard multi-rules quality control method could help analyze the type of errors. From the third to fortieth test, 186 samples were completed and the tests of 9 samples among 2 batches needed to be re-done because of the failure of quality control. The test results of other 177 samples were acceptable. CONCLUSION: The combination of the crubbs method, Z-scores figure and Westgard multi-rules quality control method can effectively accomplish the indoor quality control of MPAIA antibody test of Schistosoma japonicum.