Composite Hydrogel of Poly(vinyl alcohol) Loaded by Citrus hystrix Leaf Extract, Chitosan, and Sodium Alginate with In Vitro Antibacterial and Release Test.

Citrus hystrix leaves have been used traditionally as a spice, a traditional medicine for respiratory and digestive disorders, and a remedy for bacterial infections. This study reports on the synthesis of composite hydrogels using the freeze-thaw method with poly(vinyl alcohol) (PVA) as the building block loaded by C. hystrix leaf extract (CHLE). Additionally, chitosan (CS) and sodium alginate (SA) were also loaded, respectively, to increase the antibacterial activity and to control the extract release of the composite hydrogels. The combinations of the compositions were PVA, PVA/CHLE, PVA/CHLE/CS, PVA/CHLE/SA, and PVA/CHLE/SA/CS. The internal morphology of the hydrogels shows some changes after the PVA/CHLE hydrogel was loaded by CS, SA, and SA/CS. The analysis of the Fourier transform infrared (FTIR) spectra confirmed the presence of PVA, CHLE, CS, and SA in the composite hydrogels. From the X-ray diffraction (XRD) characterization, it was shown that the composite hydrogels maintained their semicrystalline properties with decreasing crystallinity degree after being loaded by CS, SA, and SA/CS, as also supported by differential scanning calorimetry (DSC) characterization. The compressive strength of the PVA/CHLE hydrogel decreases after the loading of CS, SA, and SA/CS, so that it becomes more elastic. Despite being loaded in the composite hydrogels, the CHLE retained its antibacterial activity, as evidenced in the in vitro antibacterial test. The loading of CS succeeded in increasing the antibacterial activity of the composite hydrogels, while the loading of SA resulted in the decrease of the antibacterial activity. The release of extract from the composite hydrogels was successfully slowed down after the loading of CS, SA, and SA/CS, resulting in a controlled release following the pseudo-Fickian diffusion. The cytotoxic activity test proved that all hydrogel samples can be used safely on normal cells up to concentrations above 1000 µg/mL.

Physicochemical Characteristics and Antibacterial Activities of Freeze-Thawed Polyvinyl Alcohol/Andrographolide Hydrogels.

Andrographolide (AG) is one of the compounds in Andrographis paniculata, which has a high antibacterial activity. This paper reports the freeze-thaw method's use to synthesize polyvinyl alcohol (PVA) hydrogels loaded with AG and its characterization. From the morphological examination, the porosity of the PVA/AG hydrogel was found to increase with the increasing AG concentration. The swelling degree test revealed that the hydrogels' maximum swelling degrees were generally greater than 100%. The composite hydrogel with the highest fraction of andrographolide (PAG-4) showed greater weight loss than the hydrogel without AG (PAG-0). The molecular interaction between PVA and AG resulted in the narrowing of the band attributed to the O-H and C=O stretching bonds and the emergence of an amorphous domain in the composite hydrogels. The loading of AG disrupted the formation of hydroxyl groups in PVA and interrupted the cross-linking between PVA chains, which lead to the decrease of the compression strength and the crystallinity increased with increasing AG. The antibacterial activity of the composite hydrogel increased with increasing AG. The PAG-4 hydrogel had the highest antibacterial activity of 37.9 ± 4.6b %. Therefore, the PVA/AG hydrogel has the potential to be used as an antibacterial device.

A freeze-thaw PVA hydrogel loaded with guava leaf extract: physical and antibacterial properties.

A polyvinyl alcohol (PVA) hydrogel loaded with guava leaf extract (GLE) has potential applications as a wound dressing with good antibacterial activity. This study succeeded in fabricating a PVA hydrogel containing GLE using the freeze-thaw (FT) method. By varying the GLE concentration, we can adjust the physical properties of the hydrogel. The addition of GLE results in a decrease in cross-linking during gelation and an increase in the pore size of the hydrogels. The increase of the pore size made the swelling increase and the mechanical strength decrease. The weight loss of the hydrogel also increases because the phosphate buffer saline (PBS) dissolves the GLE. Increasing the GLE concentration caused the Fourier-transform infrared (FTIR) absorbance peaks to widen due to hydrogen bonds formed during the FT process. The crystalline phase was transformed into an amorphous phase in the PVA/GLE hydrogel based on the X-ray diffraction (XRD) spectra. The differential scanning calorimetry (DSC) characterization showed a significant decrease in the hydrogel weight over temperatures of 30-150 °C due to the evaporation of water from the hydrogel matrix. The zone of inhibition of the PVA/GLE hydrogel increased with antibacterial activity against Staphylococcus aureus of 17.93% per gram and 15.79% per gram against Pseudomonas aeruginosa.