RESUMO
Point-of-care testing (POCT), as a portable and user-friendly technology, can obtain accurate test results immediately at the sampling point. Nowadays, microfluidic paper-based analysis devices (µPads) have attracted the eye of the public and accelerated the development of POCT. A variety of detection methods are combined with µPads to realize precise, rapid and sensitive POCT. This article mainly introduced the development of electrochemistry and optical detection methods on µPads for POCT and their applications on disease analysis, environmental monitoring and food control in the past 5 years. Finally, the challenges and future development prospects of µPads for POCT were discussed.
RESUMO
Balantidium coli is a cosmopolitan parasitic-opportunistic pathogen that can be found throughout the world. However, little information is available about prevalence of B. coli in pigs in China. In the present study, the prevalence of B. coli in pigs was investigated in Hunan province, subtropical China, between January 2012 and August 2014. A total of 3925 diarrheic fecal samples from nine representative administrative regions in Hunan province, subtropical China, were examined for the presence of B. coli cysts and/or trophozoites using microscopy after sedimentation with water. The overall prevalence of B. coli in pigs was 36.9 % (1450/3925). The present survey revealed high circulation of B. coli in pigs in Hunan province, subtropical China, which poses potential threats to human health. The results of the present investigation have important implications for the control of B. coli infections in pigs in Hunan province, subtropical China. To our knowledge, this is the first comprehensive report of B. coli prevalence in sows in Hunan province, subtropical China.
