RESUMO
We investigated the polymorphism of five X-chromosomal short tandem repeat markers (ChrX STRs) loci (DXS7132, DXS981, DXS6803, DXS6809, and DXS6789) and their value for forensic applications. A fluorescent multiplex polymerase chain reaction (PCR) for amplifying five ChrX STRs loci in the same PCR reaction was set up. A total of 827 unrelated individuals of the Han nationality in China were tested. The results show that the five loci in the multiplex system provide high polymorphism information for forensic identification and paternity testing, particularly for difficult paternity-deficient cases.
Assuntos
Cromossomos Humanos X , Impressões Digitais de DNA/métodos , Genética Populacional , Sequências de Repetição em Tandem , China , Etnicidade/genética , Feminino , Marcadores Genéticos , Humanos , Masculino , Reação em Cadeia da Polimerase/métodos , Polimorfismo GenéticoRESUMO
A fluorescent multiplex PCR that simultaneously amplifies five X-chromosomal short tandem repeat (X-STR) loci (DXS6803, DXS981, DXS6809, DXS6789 and DXS7132)was set up to study their polymorphic nature and to determine its use in kinship tests for forensic cases. PCR products were analyzed using capillary electrophoresis and ABI prism 3100 Genetic Analyzer with GeneMapper ID 3.1 Analysis Software. The pentaplex system gave satisfactory results as its sensitivity, reproducibility and unambiguous genotyping. About 20 ng DNA was routinely used, although 0.25 ng DNA was sufficient for allele typing. The results demonstrate that the multiplex system of the five X-STR loci provides a fast technology for forensic identification and paternity testing. The X-STR pentaplex system can complement the analysis of AS-STR and Y-STR efficiently, especially in complex cases of kinship testing.
Assuntos
Cromossomos Humanos X/genética , Genética Forense/métodos , Repetições de Microssatélites , Alelos , Feminino , Fluorescência , Genótipo , Humanos , Masculino , Reação em Cadeia da Polimerase , IrmãosRESUMO
To investigate the subpopulation structure within the Han Population in Guandong area, a total of 471 DNA samples from five populations in Guandong Province, including Guangzhou, Foshan, Dongguan, Jiangmen and Zhongshan-Zhuhai region, were genotyped at 15 STR(short tandem repeats) markers. Hardy-Weinberg tests were performed, allele frequencies were compared, and the genetic coancestry coefficient(FST) was estimated. The results did not show significant departure from Hardy-Weinberg equilibrium in the total population. Difference of allele frequencies among these populations was not observed, and the coancestry coefficient(FST)was less than 0.01. Subpopulation structure within Han Population in Guandong Province could not be detected.
