Effects of Shenling Chengqi Decoction on Gastrointestinal Function and Immune Status of Patients with Gastrointestinal Injury in Severe Sepsis.

Objective: To investigate the effects of Shenling Chengqi decoction combined with routine treatment on gastrointestinal function and immune status of patients with gastrointestinal injury in severe sepsis. Methods: 160 patients with sepsis were randomly divided into a control group (n = 80) and observation group (n = 80). The control group was given routine treatment such as anti-infection and vasoactive drugs. The observation group was treated with Shenling Chengqi decoction on the basis of routine treatment for 7 days. The improvement of gastrointestinal symptoms, nutritional status, gastrointestinal dynamics, inflammatory response, and immune function was observed before and after treatment. The outcome indicators were evaluation of gastrointestinal symptoms, evaluation of disease severity, nutritional status, gastrointestinal dynamic indexes, inflammatory indexes, and immune function indexes. SPSS 22.0 statistical software was used for data processing. The paired t-test was used for comparison before and after treatment. Results: Compared with the control group, the disappearance time of reflux, vomiting, and recovery time of bowel sounds in the observation group were significantly shorter (P < 0.05), and the number of defecation per day was significantly higher than that in the control group (P < 0.05). After 3 and 7 days of treatment, the APACHE II score, SIRS score, serum gas, IgA, IgM, serum hs-CRP, TNF, and PCT of the two groups were significantly lower than those before treatment (P < 0.05), and the scores of the observation group were significantly lower than those of the control group (P < 0.05). The levels of SS and MOT were significantly higher than those before treatment (P < 0.05). After 7 days of treatment, the levels of serum Hb, ALB, and PA in the two groups were significantly higher than those before treatment (P < 0.05), and the nutritional status in the observation group was significantly higher than that in the control group (P < 0.05). Conclusion: Shenling Chengqi decoction can effectively improve gastrointestinal symptoms, nutritional status, and gastrointestinal dynamics in patients with sepsis. It has high safety and is worthy of clinical promotion.

Experimental study of therapy of bone marrow mesenchymal stem cells or muscle-like cells/calcium alginate composite gel for the treatment of stress urinary incontinence.

OBJECTIVE: To explore the myoblast formation around the urethra and increase in urethral resistance of bone marrow mesenchymal stem cells or muscle-like cells/calcium alginate composite gel injection therapy and effect on LPP in SUI rat model. METHODS: Isolation, cultivation, and identification of SD rat bone marrow mesenchymal stem cell were performed. 5-Azacytidine was introduced to induce muscle-like cells. SUI was produced in 72 6-week-old female Sprague-Dawley rats, which were divided into four groups: stem cell-gel group, muscle-like cell-gel group, Gel group, and mock control group. One, 4, and 8 weeks after injection, the leak point pressure (LPP) was measured. HE staining of Desmin and α-skeletal muscle actin (α-SMA) were performed. RESULTS: At 4 and 8 weeks after injection in stem cell-gel group and muscle-like cell-gel group, growth of blood vessels gradually increased at gel edge, BMSC, and muscle-like cells gathered around the new blood vessels observed by fluorescence tracer, muscle-like cells grew into elongated spindle-like cells, Desmin, and α-SMA staining were obviously positive expression. LPP determinations of the mock control group compared with the Gel groups were significantly different. CONCLUSIONS: Compound of BMSC, muscle-like cells, and calcium alginate composite gel has the potential to differentiate into muscle cells in the microenvironment of SUI rat model. It is found by LPP measurement that the correlation between the increase in urethral resistance and the volume effect of calcium alginate gel is high.