Enhanced degradation of extracellular polymeric substances by yeast in activated sludge to achieve sludge reduction.

Candida Tropicalis was used to improve the dewaterability of activated sludge (AS) and reduce its biomass by degrading EPS in AS. The protein, polysaccharide, and hydrophilic amino acids in EPS decreased by 54.50, 29.20, and 61.01%, respectively. Meanwhile, molecular weight distribution indicated that yeast degraded macromolecular organics into small molecular ones. The direct addition of yeast to AS was more conducive to EPS degradation. With the addition of 0.75 g/L of wet yeast cells and 24 h of aeration enhanced the dewaterability of AS. The CST and MLSS decreased by 24.44 and 10.51%, respectively. After 30 days of operation of lab-scale continuous SBRs, the CST and MLSS of AS were reduced by 6.37 ± 2.01 and 3.57 ± 0.52%, respectively. FTIR spectroscopy results showed that some hydrophilic functional groups were reduced. This study provides a new approach for the in-situ reduction of AS in wastewater treatment plant.

Contributions of Various Cd(II) Adsorption Mechanisms by Phragmites australis-Activated Carbon Modified with Mannitol.

Due to its high toxicity, persistence, and bioaccumulation in the food chain, controlling cadmium (Cd) pollution in wastewater is urgent. Activated carbon is a popular material for removing Cd. To improve the Cd(II) adsorption efficiency by increasing the number of oxygen-containing functional groups, Phragmites australis-activated carbon (PAAC) was modified with mannitol at a low temperature (150 °C). The textural and chemical characteristics of PAAC and modified PAAC (M-PAAC) were analyzed by surface area analysis, elemental analysis, Boehm's titration, Fourier-transform infrared spectroscopy, and X-ray photoelectron spectroscopy. Batch adsorption experiments were conducted to investigate the influence of Cd(II) concentration, contact time, ionic strength, and pH on Cd(II) adsorption. The main adsorption mechanisms of Cd(II) on activated carbon were quantitatively calculated. The results showed that mannitol modification slightly decreased the S BET (5.30% of PAAC) and increased the content of carboxyl, lactone, and phenolic groups (total increase of 43.96% with PAAC), which enhanced the adsorption capacity of PAAC by 58.59%. The adsorption isotherms of PAAC and M-PAAC were described well using the Temkin model, while the intraparticle diffusion model fitted the Cd(II) adsorption kinetics best. Precipitation with minerals was a crucial factor for Cd(II) adsorption on activated carbon (50.40% for PAAC and 40.41% for M-PAAC). Meanwhile, the Cd(II) adsorption by M-PAAC was also dominated by complexation with oxygen-containing functional groups (33.60%). This research provides a method for recovering wetland plant biomass to prepare activated carbon and efficiently treat Cd-containing wastewater.

Single and combined impacts of nickel and cadmium on the performance, microbial community and enzymatic activity of sequencing batch reactors.

The performance, microbial enzymatic activities and the microbial community of sequencing batch reactors (SBRs) were evaluated under the single and combined nickel (Ni2+) at 20 mg/L and cadmium (Cd2+) at 10 mg/L. The single and combined Ni2+ and Cd2+ had no adverse impacts on the COD removal, whereas the NH4+-N removal efficiency declined sharply from about 99% to 34.42% and 42.67% under the single Ni2+ and combined Ni2+ and Cd2+. Compared with the absence of Ni2+ or Cd2+, the specific oxygen uptake rate (SOUR), ammonia-oxidizing rate (SAOR), nitrite-oxidizing rate (SNOR), nitrite-reducing rate (SNIRR) and nitrate-reducing rate (SNRR) declined by 24.09%, 56.63%, 51.50%, 58.01% and 52.09% under the combined Ni2+ and Cd2+, which were slower than the sum of those under single Ni2+ and Cd2+. The dehydrogenase, ammonia monooxygenase, nitrite oxidoreductase, nitrate reductase and nitrite reductase activities showed the similar varying trends to the SOUR, SAOR, SNOR, SNIRR and SNRR, suggesting that the combined Ni2+ and Cd2+ displayed antagonistic inhibition on the nitrogen removal rates and microbial enzyme activities. The combined Ni2+ and Cd2+ declined the microbial diversity and richness less than the sum of those under single Ni2+ and Cd2+. The relative abundance of Nitrosomonas, Nitrospira and identified denitrifying bacteria displayed some changes under single and combined Ni2+ and Cd2+. These findings would contribute to better understand the combined impacts of multiple heavy metals on biological wastewater treatment systems.

Enhancement of nitrogen removal by supplementing fluidized-carriers into the aerobic tank in a full-scale A2/O system.

Fluidized-carriers were supplemented into the aerobic tank of a full-scale wastewater treatment plant (WWTP) using an anaerobic/anoxic/aerobic (A2/O) system to improve the nitrogen removal efficiency in effluents. The effects of carrier supplementation on denitrification ability and the bacterial community structures were investigated over 10 months. The results showed that the average effluent concentration of total nitrogen (TN) was maintained at 9.46 ±â€¯1.14 mg/L, which was lower than 15.17 ±â€¯2.00 mg/L in the effluent without carrier supplementation, indicating that adding fluidized-carriers into the aerobic tank contributed to nitrogen removal efficiency. A thick biofilm was formed after 4 months, which provided a good anoxic-aerobic microenvironment to the microbes. Illumina sequencing analysis showed a higher bacterial diversity in the biofilm. The relative abundance of nitrifying bacteria, denitrifying bacteria, and aerobic denitrifying bacteria in the biofilms was 13.68-39%, 11.56-12.17%, and 9.76-12.50%, respectively, which was beneficial for nitrogen removal in the system. The most prevalent genera were Nitrospira, Bacillus, Thauera, Hyphomicrobium, Acinetobacter, Zoogloea, Pseudomonas, and Paracoccus, which can metabolize nitrogenous or aromatic compounds and were the major functional bacterial genera, suggesting that these organisms play key roles in biodegradation processes in the carrier-added A2/O wastewater treatment system.

Nitrogen aeration alters the spatial distribution and metal adsorption of extracellular polymeric substances in waste-activated sludge.

Extracellular polymeric substances (EPS) extracted from waste-activated sludge (WAS) have the potential to remove heavy metal ions from wastewater; both the spatial distribution and metal adsorption of EPS from WAS after nitrogen aeration were systematically investigated in this study. Compared with air aeration, nitrogen aeration significantly improved the contents of proteins (PN) and polysaccharides (PS) in the Slime-EPS (S-EPS) and loosely-bound EPS (LB-EPS), significantly increased the PS content, and slightly increased the PN content in the tightly-bound EPS (TB-EPS). The variations in the fluorescence intensities (FI) of the peaks I and II for the S-EPS, LB-EPS and TB-EPS were basically consistent with the abovementioned variations in the concentrations of these EPS. Notably, nitrogen aeration dramatically improved the content of protein-like substances in the LB-EPS. For the same aeration time, the Pb2+ reclamation rates obtained by the LB-EPS extracted from the nitrogen-aerated WAS were much higher than those achieved by the LB-EPS extracted from the air-aerated WAS. The FTIR analyses further indicated that nitrogen aeration improved the contents of the functional groups, especially -OH, -COOH and -NH2, responsible for binding heavy metals, in both the LB-EPS and TB-EPS. The SEM analyses verified that the nitrogen scours contributed to the EPS release, and Pb2+ reclamation was achieved via the attachment of Pb2+ onto the edge of the EPS. The influences of the nitrogen aeration on the spatial distribution and metal adsorption of the EPS in WAS were revealed for the first time in this study. Thus, this study lays the foundation for the application of nitrogen aeration in the resource utilization of WAS.

Influencing characteristics of short-time aerobic digestion on spatial distribution and adsorption capacity of extracellular polymeric substances in waste activated sludge.

The spatial distribution and adsorption capacity of extracellular polymeric substances (EPS) were systematically investigated for waste activated sludge (WAS) treated by a short-time aerobic digestion (STAD) process. During the STAD process, both the slime and the loosely bound EPS (LB-EPS) increased first and then decreased, while the tightly bound EPS (TB-EPS) gradually increased all the way. The variations of the fluorescence intensities at peaks A and B for the slime, the LB-EPS and the TB-EPS were consistent with the above variation of their concentrations. In the initial stage of the STAD process, aeration scour was mainly responsible for the release of LB-EPS and TB-EPS, which further resulted in the increase of slime and LB-EPS. Meanwhile, newly secreted EPS by microorganisms led to the increase of TB-EPS. In the later stage of the STAD process, the biodegradation of soluble organic matter caused the concentration decrease of both slime and LB-EPS. The Pb2+ adsorption capacity by LB-EPS, TB-EPS and total EPS gradually increased with the increasing of STAD time, suggesting that the LB-EPS and TB-EPS played a key role in the adsorption of Pb2+ and the LB-EPS had a higher adsorption capacity. FTIR results further indicated that STAD could improve the amounts of functional groups, especially for -OH, -COOH and -NH2 in both LB-EPS and TB-EPS responsible for the binding of heavy metals, resulting in the increased adsorption capacity of EPS. Disclosing the influencing characteristics of the STAD process on the spatial distribution and the adsorption capacity of EPS in WAS for the first time, this work lays a foundation for the application of the STAD process in the reuse of WAS.

New insights into biodrying mechanism associated with tryptophan and tyrosine degradations during sewage sludge biodrying.

Sewage sludge biodrying is a treatment that uses bio-heat generated from organic degradation to remove water from sewage sludge. Dewatering is still limited during biodrying, due to the presence of extracellular polymeric substances (EPS) in sludge. To study the biodrying mechanism associated with EPS compositions tryptophan and tyrosine degradations, this study investigated the microbial function in sludge biodrying material. This study conducted a taxonomic analysis of biodrying material; determined the most abundant genetic functions; analyzed the functional microorganisms involved in the degradations of tryptophan and tyrosine; and summarized the metabolic pathways. The results indicated efficient degradations of tryptophan and tyrosine were observed during the initial thermophilic phase; functional microorganisms were mainly from the phyla Firmicutes, Actinobacteria, and Proteobacteria, enriched with genes involved in amino acid transport and metabolism. These findings highlight the potentially important microorganisms and typical pathways that may help improve dewaterability during biodegradation.

Impacts of cell surface characteristics on population dynamics in a sequencing batch yeast reactor treating vegetable oil-containing wastewater.

Ten yeast strains acquired from different sources and capable of utilizing vegetable oil or related compounds (fatty acid or oleic acid) as sole carbon sources were inoculated into a sequencing batch reactor (SBR) for the treatment of high-strength vegetable oil-containing wastewater. The SBR system stably removed >89% of chemical oxygen demand (COD) and >99% of oil when fed with wastewater containing 15 g/L COD and 10 g/L oil in average. Denaturing gradient gel electrophoresis of polymerase chain reaction-amplified 26S rRNA genes showed that among the ten yeast strains, only Candida lipolytica, Candida tropicalis, and Candida halophila were dominant in the system. To elucidate the major factors affecting the selection of yeast strains in the SBR system, the three dominant strains were compared with two non-dominant strains in terms of COD removal performance, biomass yield, cell settleability, cell flocculation ability, cell emulsification ability, and surface hydrophobicity. Results showed that hydrophobicity and emulsification ability of yeast cells were the two most important factors determining the selection of yeast strains in the treatment of high-strength oil-containing wastewater.

Analysis of bacterial community structures in two sewage treatment plants with different sludge properties and treatment performance by nested PCR-DGGE method.

The bacterial community structures in two sewage treatment plants with different processes and performance were investigated by denaturing gradient gel electrophoresis (DGGE) of nested polymerase chain reaction (nested PCR) amplified 16S rRNA gene fragments with group-specific primers. Samples of raw sewage and treated effluents were amplified using the whole-cell PCR method, and the activated sludge samples were amplified using the extracted genomic DNA before the PCR products were loaded on the same DGGE gel for bacterial community analysis. Ammonia-oxidizing bacterial and actinomycetic community analysis were also carried out to investigate the relationship between specific population structures and system or sludge performance. The two plants demonstrated a similarity in bacterial community structures of raw sewage and activated sludge, but they had different effluent populations. Many dominant bacterial populations of raw sewage did not appear in the activated sludge samples, suggesting that the dominant bacterial populations in raw sewage might not play an important role during wastewater treatment. Although the two plants had different sludge properties in terms of settleability and foam forming ability, they demonstrated similar actinomycetic community structures. For activated sludge with bad settling performance, the treated water presented a similar DGGE pattern with that of activated sludge, indicating the nonselective washout of bacteria from the system. The plant with better ammonium removal efficiency showed higher ammonia-oxidizing bacteria species richness. Analysis of sequencing results showed that the major populations in raw sewage were uncultured bacterium, while in activated sludge the predominant populations were beta proteobacteria.

Virus removal performance and mechanism of a submerged membrane bioreactor.

Responding to the worldwide outbreak of SARS in 2003, virus removal performance and mechanism of a SMBR were investigated by employing phage T4 as a model virus. Two membrane modules were compared in continuous operation for about 75 days. During stable operation, SMBR achieved almost complete phage removal for both membrane modules. For the 0.22 µm module, the cake layer, the gel layer and the membrane contributed 6.3 log, 3.1 log and 1.7 log, respectively to phage removal, confirming the importance of the cake/gel layer formed on the surface of membrane. The damage of the cake/gel layer resulted in the decrease of phage removal. As for the 0.1 µm one, the membrane alone played a major role in phage removal. Inactivation by activated sludge and adsorption by cake/gel layer contributed about 3.6 log to phage removal everyday so that there was no phage accumulation in bulk solution. The results demonstrated that SMBR was an efficient system and recommended for treatment of virus-bearing wastewater.