RESUMO
Langerhans cell histiocytosis (LCH) is a rare monoclonal disease,its clinical presentation is highly variable because it can affect multiple organs, such as lung, bone, skin, lymph nodes, hypothalamopituitary axis, and other multiple sites. LCH involving the thyroid gland is extremely rare, here we reported a rare case of LCH involving thyroid, presenting as painless thyroid goiters.
Assuntos
Histiocitose de Células de Langerhans/diagnóstico , Histiocitose de Células de Langerhans/patologia , Doenças da Glândula Tireoide/patologia , Glândula Tireoide/patologia , Histiocitose de Células de Langerhans/complicações , Histiocitose de Células de Langerhans/cirurgia , Humanos , Doenças da Glândula Tireoide/cirurgia , Glândula Tireoide/cirurgia , Tireoidectomia , Resultado do TratamentoRESUMO
BACKGROUND: Proteomics tools can be used to identify the differentially expressed proteins related to allergic rhinitis (AR). However, the large numbers of proteins related to AR have not yet been explored using an advanced quantitative proteomics approach, known as isobaric tags for relative and absolute quantitation (iTRAQ). OBJECTIVES: To identify differentially expressed proteins in persistent AR patients and to explore the regulatory signalling pathways involving the identified proteins. METHODS: Forty-five persistent AR patients and 20 healthy controls were recruited for this study. iTRAQ was used to identify the proteins that were differentially expressed between these two groups, and a bioinformatics analysis was then conducted to identify the signalling pathways associated with the identified proteins. Immunofluorescence labelling was performed to detect alpha-2-macroglobulin (A2M), STAT3, p-STAT3 and IL17 in the nasal mucosa. RESULTS: A total of 133 differentially expressed proteins were identified. We then determined the top 10 regulatory pathways associated with these proteins and found that the blood coagulation pathway had the most significant association. A2M, a protein involved in the blood coagulation pathway, was found to be differentially expressed in the serum of AR patients. The bioinformatics analysis indicated that STAT3 is an upstream transcription factor that might regulate A2M expression. An immunofluorescence study further confirmed that STAT3 and A2M are co-localized in nasal mucosa cells. Additionally, A2M, STAT3, p-STAT3, and IL17 are elevated in AR patients. The expressional level of A2M is positively related to IL17 and the symptom of the congestion in AR subjects. CONCLUSIONS: The blood coagulation pathway may be a key regulatory network pathway contributing to the allergic inflammatory response in AR patients. A2M, which is regulated by STAT3, may be an important protein in the pathogenesis of allergic rhinitis in AR patients.
Assuntos
alfa 2-Macroglobulinas Associadas à Gravidez/metabolismo , Proteoma , Proteômica , Rinite Alérgica/imunologia , Rinite Alérgica/metabolismo , Fator de Transcrição STAT3/metabolismo , Adulto , Coagulação Sanguínea , Estudos de Casos e Controles , Biologia Computacional/métodos , Feminino , Humanos , Masculino , Ligação Proteica , Mapeamento de Interação de Proteínas , Mapas de Interação de Proteínas , Proteômica/métodos , Reprodutibilidade dos Testes , Rinite Alérgica/sangue , Rinite Alérgica/diagnóstico , Transdução de Sinais , Testes CutâneosRESUMO
We report the synthesis of (Ca0.33Na0.66)Fe2As2 showing a superconducting transition with T(c) above 33 K. Both dc magnetic susceptibility or specific heat measurements indicated the bulk superconductivity nature of the sample. We also have successfully grown single crystals of the (Ca0.33Na0.66)Fe2As2 superconductors. The single crystals exhibit sharp superconducting transitions with T(c) above 33 K. The effects of magnetic field on the superconducting transitions are studied, giving rise to high upper critical fields with H(c2)(c)≈85 T and H(c2)(ab)≈172 T, respectively. The anisotropy parameter was calculated to be around 2.
