RESUMO
Four new inter-nucleotide distance sequences for a DNA sequence are defined. They are different from ones presented by Afreixo et al., and overcome the irreversible defect of the global inter-nucleotide distance sequence proposed by Nair and Mahalakshmi. Five basic statistical quantities are extracted from (ordered) precise inter-nucleotide distance sequences to construct a 20 dimensional feature vector. This simple mathematical descriptor of DNA sequence plays crucial roles in sequence comparison and essential gene identification. Euclidean distance between feature vectors is utilized to compare similarities among whole mitochondrial genomes of 18 eutherian mammals and 23 sequences of 16S ribosomal RNA, respectively. Derived phylogenetic trees are quite agreement with a few popular studies. Furthermore, using feature vector as input a support vector machine (SVM)-based method are developed to identify essential genes and non-essential genes of 5 bacteria. Higher AUC values (the minimum is 0.7971, the highest reaches 0.8751 and the average is 0.8174) than some well-known results confirm the performance of the method.
Assuntos
Bactérias/genética , Mamíferos/genética , RNA Bacteriano/genética , RNA Ribossômico 16S/genética , RNA/genética , Análise de Sequência de RNA/métodos , Animais , RNA Mitocondrial , Máquina de Vetores de SuporteRESUMO
BACKGROUND: The Kidney has been the target organ for the delivery of silencing ribonucleic acids (silencing RNA) administered systemically in comparison to other body tissues. MATERIALS AND METHOD: In this review, we discussed different approaches made to delivering proteins to the kidneys in different conditions like normal and pathological defects. Data from clinical experiments have been used to discuss and support the administration of silencing RNA for the treatment of kidney diseases. RESULTS: Results were achieved using the available genome wide RNA libraries. CONCLUSION: The research results are helpful in application to 3D and conventional models to find the involvement of signal pathways in kidney diseases.
Assuntos
Terapia Genética , Nefropatias , Rim/metabolismo , Proteínas/metabolismo , Interferência de RNA , RNA , Humanos , Nefropatias/metabolismo , Nefropatias/terapiaRESUMO
The elasticity of the ascending aorta in healthy volunteers and hypertension patients were examined by using quantitative tissue velocity imaging (QTVI), and the age-related change in the ascending aortic elasticity was investigated. The anterior and posterior walls of the ascending aorta were imaged with tissue Doppler method in all the subjects and QTVI was performed. Stable curves were obtained from 173 hypertension patients and 185 healthy adults. The peak early diastolic velocity (V(e)), peak late diastolic velocity (V(a)) and peak systolic velocity (V(s)) were measured. The relation of age with these measures was assessed. The results showed that the elasticity of the ascending aorta was much lower in the hypertension patients than in normal controls (P<0.05), and the elasticity was decreased with age in both groups (P<0.05). Our results suggested that QTVI, a new non-invasive ultrasonic technique, is helpful for the assessment of the aortic elasticity in hypertension patients.
Assuntos
Aorta/diagnóstico por imagem , Técnicas de Imagem por Elasticidade/métodos , Hipertensão/fisiopatologia , Ultrassonografia Doppler em Cores/métodos , Adulto , Idoso , Aorta/fisiopatologia , Estudos de Casos e Controles , Feminino , Humanos , Hipertensão/diagnóstico por imagem , Masculino , Pessoa de Meia-IdadeRESUMO
The elasticity of the ascending aorta in healthy volunteers and hypertension patients were examined by using quantitative tissue velocity imaging (QTVI),and the age-related change in the ascending aortic elasticity was investigated. The anterior and posterior walls of the ascending aorta were imaged with tissue Doppler method in all the subjects and QTVI was performed. Stable curves were obtained from 173 hypertension patients and 185 healthy adults. The peak early diastolic velocity (Ve),peak late diastolic velocity (Va) and peak systolic velocity (Vs) were measured. The relation of age with these measures was assessed. The results showed that the elasticity of the ascending aorta was much lower in the hypertension patients than in normal controls (P＜0.05),and the elasticity was decreased with age in both groups (P＜0.05). Our results suggested that QTVI,a new non-invasive ultrasonic technique,is helpful for the assessment of the aortic elasticity in hypertension patients.