Chiral MoS2@BC fibrous membranes selectively promote peripheral nerve regeneration.

BACKGROUND: Molybdenum disulfide (MoS2) has excellent physical and chemical properties. Further, chiral MoS2 (CMS) exhibits excellent chiroptical and enantioselective effects, and the enantioselective properties of CMS have been studied for the treatment of neurodegenerative diseases. Intriguingly, left- and right-handed materials have different effects on promoting the differentiation of neural stem cells into neurons. However, the effect of the enantioselectivity of chiral materials on peripheral nerve regeneration remains unclear. METHODS: In this study, CMS@bacterial cellulose (BC) scaffolds were fabricated using a hydrothermal approach. The CMS@BC films synthesized with L-2-amino-3-phenyl-1-propanol was defined as L-CMS. The CMS@BC films synthesized with D-2-amino-3-phenyl-1-propanol was defined as D-CMS. The biocompatibility of CMS@BC scaffolds and their effect on Schwann cells (SCs) were validated by cellular experiments. In addition, these scaffolds were implanted in rat sciatic nerve defect sites for three months. RESULTS: These chiral scaffolds displayed high hydrophilicity, good mechanical properties, and low cytotoxicity. Further, we found that the L-CMS scaffolds were superior to the D-CMS scaffolds in promoting SCs proliferation. After three months, the scaffolds showed good biocompatibility in vivo, and the nerve conducting velocities of the L-CMS and D-CMS scaffolds were 51.2 m/s and 26.8 m/s, respectively. The L-CMS scaffolds showed a better regenerative effect than the D-CMS scaffolds. Similarly, the sciatic nerve function index and effects on the motor and electrophysiological functions were higher for the L-CMS scaffolds than the D-CMS scaffolds. Finally, the axon diameter and myelin sheath thickness of the regenerated nerves were improved in the L-CMS group. CONCLUSION: We found that the CMS@BC can promote peripheral nerve regeneration, and in general, the L-CMS group exhibited superior repair performance. Overall, the findings of this study reveal that CMS@BC can be used as a chiral nanomaterial nerve scaffold for peripheral nerve repair.

Influence of applied voltage on bioelectrochemical enhancement of biomethanation for low-rank coal and microbial community distribution.

The performance of peat biomethanation was investigated in bioelectrochemical anaerobic digestion at different applied voltages, and compared to conventional anaerobic digestion. The methane yield was stabilized at 16 mL/g peat in the conventional anaerobic digestion. However, in the bioelectrochemical anaerobic digestion, the methane yield was significantly increased to 264 mL/g peat at the applied voltage of 4 V, followed by 1 V, 2 V, 0.5 V and 0 V. The bioelectrochemical system could enrich more electroactive microorganisms on the electrode, as well as in the bulk solution, and further improve the direct interspecies electron transfer for methane production. The 16S rRNA analysis showed a significant increase in the abundance of specific microorganisms in the bulk solution, including Firmicutes phylum and Proteobacteria phylum, in addition to a gradual increase in acetoclastic methanogenesis with an increase in applied voltage. These results provide a solution to turn low-rank coal into a new alternative energy.

Contribution analysis of different electron transfer pathways to methane production in anaerobic digestion coupled with bioelectrochemical system.

The contribution analysis of different electron transfer pathways to CH4 production was investigated in bioelectrochemical anaerobic digestion (BEAD). It demonstrates that the indirect interspecies electron transfer (IIET) pathway and the direct interspecies electron transfer (DIET) pathways contributed to 41.7 % and 58.3 % of the CH4 production in the BEAD reactor, respectively. The DIET pathway was further divided into DIET via electrode (eDIET) and biological DIET (bDIET) in the bulk solution, and contributed 11.1 % and 47.2 % of CH4 production, respectively. This indicates that the dominant electron transfer pathway for CH4 production is from the bulk solution, rather than on the polarized electrode. The electroactive microorganisms were well enriched in the bulk solution by the electric field generated between anode and cathode. The enriched electroactive microorganisms significantly improved the CH4 production in the bulk solution through the bDIET pathway.

Performance of bioelectrode based on different carbon materials in bioelectrochemical anaerobic digestion for methanation of maize straw.

The performance of the bioelectrochemical anaerobic digestion (BEAD) reactor was investigated with different carbon material-modified electrodes for the methanation of maize straw. The carbon material-modified electrodes used titanium (Ti) mesh modified with carbon nanotube (CNT), carbon black (CB), and activated carbon (AC). The maximum cumulative methane production obtained in the Ti-CNT reactor was (616.4 ± 9.3) mL/g VS, while the maximum methane production rate in the Ti-AC reactor was (61.9 ± 1.0) mL/g VS.d.The electroactive bacteria were well enriched by the different electrodes, and the enriched electroactive bacteria further facilitate the direct interspecies electron transfer (DIET) for methane production. Additionally, we found the phylum Firmicutes showed a linear relationship to methanogenic performance, as well as the Genus Proteiniborus. The Ti-CNT electrode shows better performance by the electrochemical analysis. These findings provide critical knowledge for the large-scale use of the BEAD process and the treatment of maize straw.