RESUMO
BACKGROUND: Dysregulation of RNA guanine-7 methyltransferase (RNMT) plays a crucial role in the tumor progression and immune responses. However, the detailed role of RNMT in pan-cancer is still unknown. METHODS: Bulk transcriptomic data of pan-cancer were obtained from the Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), and Cancer Cell Line Encyclopedia (CCLE) databases. Single-cell transcriptomic and proteomics data of lung squamous cell carcinoma (LUSC) were analyzed in the Tumor Immune Single-cell Hub 2 (TISCH2) and Clinical Proteomic Tumor Analysis Consortium (CPTAC) databases, respectively. The correlation between RNMT expression and cancer prognosis was analyzed by Cox proportional hazards regression and Kaplan-Meier analyses. The correlation of RNMT expression with common immunoregulators, tumor mutation burden (TMB), microsatellite instability (MSI), mismatch repair (MMR), and DNA methyltransferase (DNMT) was analyzed. Additionally, the correlation between RNMT expression and immune infiltration level was evaluated. A total of 1287 machine learning combinations were used to construct prognostic models for LUSC. qRT-PCR and Western blot were used to validate the bioinformatics findings of RNMT upregulation in LUSC. RESULTS: RNMT was widely expressed across different cancers, with significant correlation to prognosis in cancers such as kidney chromophobe (KICH) (p = 0.0033, HR = 7.12), liver hepatocellular carcinoma (LIHC) (p = 0.01, HR = 1.41), and others. Notably, RNMT participates in the regulation of the tumor microenvironment. RNMT expression positively correlated with immune cell expression (Spearman's rank correlation, p < 0.05). Moreover, RNMT expression was strongly associated with immunoregulators, TMB, MSI, MMR, and DNMT in most cancer types. Notably, RNMT expression displayed excellent prognostic and immunological performance in LUSC. The expression of RNMT was mainly enriched in B cells of LUSC tissues. qRT-PCR and Western blot verified the high expression of RNMT in LUSC. CONCLUSION: RNMT expression widely correlated with prognosis and immune infiltration in various tumors, especially LUSC. The RNMT detection may provide a new idea for future tumor immune studies and treatment strategies.
RESUMO
Proper scaffolds combined with mesenchymal stem cells (MSCs) represent a promising strategy for repairing bone defects. In a previous study, poly (fumaroyl bioxirane) maleate (PFM), a newly developed functional polymer with numerous functional groups, exhibited excellent biocompatibility and enhanced the alkaline phosphatase (ALP) activity of osteoblasts in vitro. Here, to provide further and comprehensive insight into the application of PFM in bone tissue engineering, we investigated the osteoinductive potential of PFM cultured with rat adipose-derived mesenchymal stem cells (rADSCs). The results showed that PFM resulted in greater proliferation of rADSCs and that the PFM substrate had stronger osteoinductivity than PLGA and the control, as indicated by the significant upregulation of osteogenesis-related genes, proteins and calcium mineralization in vitro. Next, PFM was combined with rADSCs to repair a critical-sized calvarial defect in rats. Compared to the PLGA scaffold, the PFM scaffold significantly promoted new bone formation and exhibited excellent effects in repairing rat calvarial defects. In conclusion, PFM possesses strong osteoinductivity, which could markedly enhance bone regeneration, suggesting that PFM could serve as a promising and effective optimization method for traditional scaffolds in bone regeneration.
