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1.
Mol Hum Reprod ; 15(12): 805-19, 2009 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19584195

RESUMO

Prenatal oogenesis produces hundreds of thousands of oocytes, most of which are discarded through apoptosis before birth. Despite this large-scale selection, the survivors do not constitute a perfect population, and the factors at the cellular level that result in apoptosis or survival of any individual oocyte are largely unknown. What then are the selection criteria that determine the size and quality of the ovarian reserve in women? This review focuses on new data at the cellular level, on human prenatal oogenesis, offering clues about the importance of the timing of entry to meiotic prophase I by linking the stages and progress through MPI with the presence or absence of apoptotic markers. The characteristics and responsiveness of cultured human fetal ovarian tissue at different gestational ages to growth factor supplementation and the impact of meiotic abnormalities upon apoptotic markers are discussed. Future work will require the use of a tissue culture model of prenatal oogenesis in order to investigate the fate of individual live oocytes at different stages of development.


Assuntos
Morte Celular/fisiologia , Oócitos , Oogênese/fisiologia , Ovário , Animais , Feminino , Feto/anatomia & histologia , Feto/fisiologia , Humanos , Marcação In Situ das Extremidades Cortadas , Prófase Meiótica I/fisiologia , Camundongos , Oócitos/citologia , Oócitos/fisiologia , Ovário/citologia , Ovário/embriologia , Poli(ADP-Ribose) Polimerase-1 , Poli(ADP-Ribose) Polimerases/metabolismo
2.
Reprod Biomed Online ; 15(1): 99-105, 2007 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-17623546

RESUMO

To date, the effects of freezing on spermatogenesis have not yet been fully investigated at a molecular level. Antibody localization studies have identified the MutL homolog 1 (MLH1) protein, a mis-match repair protein, at the prophase I stage of meiosis, which allows the detection of recombination foci during pachytene. This study investigated the effect of long-term testicular tissue cryopreservation on meiotic prophase I, identified by recombination foci frequency and synaptonemal complex (SC) integrity. Frozen-thawed testicular tissues from 12 males who had each fathered a child were used. Because vasectomy or reverse vasectomy procedures are rare in the locale of the investigation, it was not possible to obtain fresh testicular tissue and use the males as their own controls. Immunocytogenetic analysis of 612 spermatocytes at the pachytene stage was performed. The results indicated a mean number of MLH1 foci of 49.2 (SD +/- 5.9), and no correlation was found between the freezing period, the MLH1 frequency and the SC integrity. The results suggest that freezing of testicular tissue taken post-puberty does not appear to be detrimental to the crossover process as identified by occurrence of MLH1 loci.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/genética , Troca Genética , Criopreservação , Proteínas Nucleares/genética , Espermatócitos/fisiologia , Testículo/citologia , Adulto , Imunofluorescência , Congelamento , Humanos , Infertilidade Masculina , Masculino , Meiose , Pessoa de Meia-Idade , Proteína 1 Homóloga a MutL , Prófase , Espermatócitos/citologia , Espermatogênese
3.
Mol Hum Reprod ; 8(10): 906-11, 2002 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-12356939

RESUMO

Mouse fetal ovaries were cultured to investigate germ cell development in the presence of a combination of the growth factors (GFs) stem cell factor, insulin-like growth factor-1 and leukaemia inhibitory factor. Ovaries were isolated from fetal mice at 13 and 14 days post-coitum (dpc) and cultured to the equivalent of 17 dpc. Culture conditions comprised minimal essential medium-alpha plus 5% fetal calf serum, with or without GFs. Oocytes were assessed using immunofluorescence to illustrate synaptonemal complexes and recombination foci. The proportions of pachytene cells in freshly isolated 13, 14 and 17 dpc ovaries were 0, 8 and 74% respectively. There was a significant (P < 0.0001) increase in the number of pachytene cells after 4 days culture with GFs, with 24% of germ cells from 13 dpc ovaries reaching pachytene. In contrast, no pachytene cells were detected in cultures of 13 dpc ovaries without GFs. After 3 days in culture with GFs, 38% of germ cells from 14 dpc ovaries were at pachytene compared with 19% without GFs. In conclusion, we have demonstrated positive effects of GFs upon oocyte formation by meiosis in vitro. The observed results could be explained by an increased survival of premeiotic oogonia entering meiosis, or by effects on oocytes already in early meiosis.


Assuntos
Substâncias de Crescimento/farmacologia , Meiose/efeitos dos fármacos , Ovário/embriologia , Ovário/fisiologia , Proteínas Adaptadoras de Transdução de Sinal , Animais , Proteínas de Transporte , Feminino , Idade Gestacional , Meiose/fisiologia , Camundongos , Camundongos Endogâmicos , Proteína 1 Homóloga a MutL , Proteínas de Neoplasias/metabolismo , Proteínas Nucleares , Oócitos/efeitos dos fármacos , Oócitos/fisiologia , Técnicas de Cultura de Órgãos , Ovário/efeitos dos fármacos , Recombinação Genética , Fatores de Tempo
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