[Polish preparations for preventing and treating infections caused by Pseudomonas aeruginosa]. / Pol'skie preparaty dlia profilaktiki i lecheniia infektsii, vyzvaemykh Pseudomonas aeruginosa.

Three preparations, viz. sheep serum for local application ("Sanoserum"), anti-Pseudomonas sheep immunoglobulin for passive immunization ("Immunoglobulin Pseudomonas") and Pseudomonas vaccine for active immunization ("Pseudovac"), have been obtained at the Central Laboratory of Sera and Vaccines in Warsaw, Poland. The laboratory study and clinical trial of these preparations have been carried out. The preparations have proved to be effective as supplement to the conventional methods for the early diagnosis and treatment of P. aeruginosa infection in burn patients.

Anti-Pseudomonas immunoglobulin. IV. Combined anti-Pseudomonas immunoglobulin and Pseudomonas vaccine immunotherapy of burned patients--clinical investigations.

Sheep anti-Pseudomonas immunoglobulin and Pseudomonas vaccine were used for combined immunotherapy in 55 severely burned patients. The results of clinical investigations show that active and passive immunization in the early state of thermal injury increases the anti-Pseudomonas agglutinin titer, diminish the threat of Pseudomonas sepsis and death and significantly accelerate the recovery.

Anti-Pseudomonas immunoglobulin I. Immunization of sheep.

The production of sheep Pseudomonas immune serum is described. The animals were immunized with Pseudomonas vaccine prepared from strains of Pseudomonas aeruginosa belonging to seven immunotypes of Fisher's schema. Immune sera obtained from hyperimmunized sheep are characterized by a high level of stabel antibodies with a good protective activity for mice against infection with Pseudomonas aeruginosa. It has been demonstrated that sheep may be used for a long time as a source of immune serum.

Anti-pseudomonas immunoglobulin. II. Preparation of purified sheep immunoglobulins.

Several methods of fractionation of sheep anti-sera against Pseudomonas aeruginosa were applied for the preparation of immunoglobulins. The methods involving salting out with ammonium sulfate or purification by means of caprylic acid were shown to be most suitable for isolation of immunoglobulins under manufacturing conditions. The preparations obtained by these methods agglutinated Pseudomonas aeruginosa, suspension in vitro and protected mice against lethal infection with this microorganism.