Evaluation of healing processes of intraosseous defects with and without guided bone regeneration and platelet rich plasma. An animal study.

BACKGROUND: In most cases, the natural healing of intrabony defects only leads to restoration of tissue continuity without differentiation and function. However, repair is not regarded to be an optimal treatment method, as confirmed in many clinical cases. Thus it is important to choose a surgical procedure which makes it possible to achieve restitution ad integrum of the bone structure. The choice of the GBR technique is crucial, in terms of the clinical conditions and limitations resulting from the use of a particular material. OBJECTIVE: The objective of this study has been the analysis of effectiveness of selected surgical treatment techniques of intrabony defects in rabbits. MATERIALS AND METHODS: Research was conducted on 36 white rabbits. The operation technique was a criterion of division into 3 groups: BG/BOC (Bio-Oss Collagen(®)+Bio-Gide Perio(®)), BOC/PRP (Bio-Oss Collagen(®)+PRP), C (control group). Qualitative and quantitative histopathological evaluation was carried out after 1, 3, 6 and 12 months. RESULTS: The highest value of the bone surface area 31.9% (SD 1.8) was achieved in BOC/BG group three months after the implantation, while the lowest was revealed in C - group - 12.5% (SD 1.32) one month following the procedure. CONCLUSIONS: Upon quantitative histological assessment, the bone tissue presented the most intensive osteogenesis within one month from the application of BOC/PRP, whereas this was observed after the application of BOC/BG in later stages. The application of two regenerative methods influenced the rate, quality and overall treatment of intraosseus defects.

The clinical efficacy of primary culture of human fibroblasts in gingival augmentation procedures-a preliminary report.

The treatment of mucogingival problems is one of the main objectives of periodontal treatment. The insufficient or absent gingival attachment increases the risk of development of gingival recessions. Multiple gingival augmentation techniques of varying level of success are known. The aim of this study has been to clinically and histologically evaluate the integration process of scaffolds composed of primary human fibroblasts derived from keratinized gingiva on collagenous carriers. Ten patients exhibiting a mucogingival problem and gingival inflammation of the related teeth were included in the study. In total, 34 teeth in the anterior part of the maxilla and the mandible underwent treatment. Clinically, over a 6 month period of observation, a significant decrease in the distance from cemento-enamel junction to mucogingival junction (CEJ-MGJ) was revealed. Histologically, mature connective tissue covered by keratinized epithelium was found after 12 weeks. No specimens revealed an inflammatory response. A complete clinical healing was observed after 2 weeks in eight patients [early healing index (EHI)=I]. The results of clinical evaluation indicate that the method of primary culture of human fibroblasts on a collagenous carrier for gingival augmentation is an esthetic and effective method of mucogingival complex repair. The scaffolds were substituted and completely re-epithelialized within 12 weeks according to histologic results.

Early histological evaluation of bone defect healing with and without guided bone regeneration techniques: experimental animal studies.

INTRODUCTIONS: To obtain osseous tissue regeneration, treatment methods referred to as guided bone regeneration are used which utilize the mechanical, chemical, and biological properties of materials. MATERIAL/METHODS: Eighteen white rabbits were used. Under general anesthesia, a 5 mm in diameter defect was created transcutaneously in the femur trochanter major. The rabbits were divided into three groups depending on the type of the intraosseous defect (ID) treatment: in group I (C) the control IDs were left to heal spontaneously, in II (BOC+BG) the IDs were filled with Bio-Oss Collagen and Bio Gide Perio membrane, and in III (PRP) the IDs were filled with BOC and platelet-rich plasma (Curasan Centrifuge). The animals were sacrificed 1 and 3 months after the surgical procedure. The histological material was stained with hematoxylin and eosin and using the van Gieson method. RESULTS AND CONCLUSIONS: In the earlier histological examinations (1 and 3 months after the procedures), resorption of the biomaterial and the formation of new bone trabeculas were observed in both groups II and III. At the first observation the extent of biomaterial resorption and the intensity of the osteogenic process were greater in group III, but after 3 months group II had a slight advantage. Fragmented remnants of the biomaterial in both groups were surrounded by newly formed bone and locally by fibrous connective tissue. At both observation times the number of bone trabeculas after implantation in groups II and III was greater than in the control group.