Bioelectrochemical assisted dechlorination of tetrachloroethylene and 1,2-dichloroethane by acclimation of anaerobic sludge.

Volatile chlorinated hydrocarbons (VCHs) are often found as a type of persistent and ubiquitous contaminant in groundwater. The feasibility, characteristics and microbial mechanism of acclimation of biodiversity-rich inoculation source for bioelectrochemical stimulated VCH dechlorination remain poorly understood. Here, the superior bioelectrochemical catalytic activities were observed for tetrachloroethylene (0.26 mM d-1) and 1,2-dichloroethane (2.20 mM d-1) dechlorination in anaerobic sludge-acclimated biocathodes with an optimal potential of -0.5 V, averaging 1.60-2.71 times higher than those reported in previous works on biocathodes. When the cathode was applied as the sole electron donor for dechlorination, columbic efficiencies reached the values greater than 80%. Tetrachloroethylene dechlorination showed a metabolic pathway with cis-1,2-dichloroethene as the main product, whereas 1,2-dichloroethane was dechlorinated entirely to the nontoxic ethene. The cathodic biofilms were highly abundant with the dechlorination and electro-active genera, while significant bacterial consortium variation was observed in response to the different VCH types and changes in cathodic potential. Bacillus, Pseudomonas and Lactococcus were mostly enriched for tetrachloroethylene dechlorination, and pceA, tceA and omcX were highly expressed. Geobacter was the most predominant during 1,2-dichloroethane dechlorination with rdhA, tceA and omcX highly expressed. In addition, although the impact of cathodic potentials was weaker than that of VCH types, the lower cathodic potentials, the more abundant of the electrode respiring populations and the higher expression of extracellular electron transfer related gene. This study demonstrated the great potential of acclimation of anaerobic sludge by electrical stimulation for accelerating VCH remediations and gave insights into its working molecular mechanisms.

Environmentally persistent free radicals in PM2.5: a review.

Environmentally persistent free radicals (EPFRs) are a new class of pollutants that are long-lived in fine particles (PM2.5), i.e., their 1/e lifetime ranges from days to months (or even infinite). They are capable of producing harmful reactive oxygen species such as hydroxyl radicals. The redox cycling of EPFRs is considered as an important pathway for PM2.5 to induce oxidative stress inside the humans, causing adverse health effects such as respiratory and cardiovascular diseases. Consequently, research regarding their toxicity, formation and environmental occurrences in PM2.5 has attracted increasing attentions globally during the past two decades. However, literature data in this field remain quite limited and discrete. Hence, an extensive review is urgently needed to summarize the current understanding of this topic. In this work, we systematically reviewed the analytical methods and environmental occurrences, e.g., types, concentrations, and decay behaviors, as well as possible sources of EPFRs in PM2.5. The types of pretreatment methods, g-values of common EPFRs and categories of decay processes were discussed in detail. Moreover, great efforts were made to revisit the original data of the published works of EPFRs in airborne particulate matter and provided additional useful information for comparison where possible, e.g., their mean and standard deviation of g-values, line widths (ΔH p-p), and concentrations. Finally, possible research opportunities were highlighted to further advance our knowledge of this emerging issue.