Changes in the structure and composition of pineapple leaf fiber after alkali and ionic surfactant pretreatments and their impact on enzymatic hydrolysis.

The current study investigated the effects of two different pretreatments (NaOH and alkaline surfactant assisted) on the chemical, morphological and enzymatic saccharification of pineapple leaf fiber (PALF). Results showed that both pretreatments significantly reduced lignin content of the biomass, achieving a 69.6 and a 76.3% reduction for NaOH and surfactant pretreated materials, respectively. SEM, CLSM and FTIR-ATR techniques were used to evaluate morphological changes in the fibers after pretreatments. Images obtained revealed cellulose exposure and lignin redistribution in the pretreated fibers. Surfactant pretreated material provided the best results after enzymatic hydrolysis compared to NaOH and untreated PALF. A final enzymatic hydrolysis yield of 81.8% was obtained after a 24 h process using surfactant pretreated fibers, in comparison to 75.9 and 45.1% yields for NaOH pretreated material and raw fibers, respectively. Nowadays, the use of agricultural residues for high added value products is of great importance for sustainable development. This work specifically studied an effective and green approach for lignin removal and enzymatic hydrolysis from pineapple leaf fiber that is an abundant waste in Costa Rica and an interesting feedstock for biorefinery processes design.

Addition of Surfactants and Non-Hydrolytic Proteins and Their Influence on Enzymatic Hydrolysis of Pretreated Sugarcane Bagasse.

Poly(ethylene glycol) (PEG 4000) and bovine serum albumin (BSA) were investigated with the purpose of evaluating their influence on enzymatic hydrolysis of sugarcane bagasse. Effects of these supplements were assayed for different enzymatic cocktails (Trichoderma harzianum and Penicillium funiculosum) that acted on lignocellulosic material submitted to different pretreatment methods with varying solid (25 and 100 g/L) and protein (7.5 and 20 mg/g cellulose) loadings. The highest levels of glucose release were achieved using partially delignified cellulignin as substrate, along with the T. harzianum cocktail: increases of 14 and 18 % for 25 g/L solid loadings and of 33 and 43 % for 100 g/L solid loadings were reached for BSA and PEG supplementation, respectively. Addition of these supplements could maintain hydrolysis yield even for higher solid loadings, but for higher enzymatic cocktail protein loadings, increases in glucose release were not observed. Results indicate that synergism might occur among these additives and cellulase and xylanases. The use of these supplements, besides depending on factors such as pretreatment method of sugarcane bagasse, enzymatic cocktails composition, and solid and protein loadings, may not always lead to positive effects on the hydrolysis of lignocellulosic material, making it necessary further statistical studies, according to process conditions.

Design of an enzyme cocktail consisting of different fungal platforms for efficient hydrolysis of sugarcane bagasse: Optimization and synergism studies.

Lignocellulosic materials represent a very important and promising source of renewable biomass. In order to turn them into fermentable sugars, synergism among the different enzymes that carry out bioconversion of these materials is one of the main factors that should be considered. Experimental mixture design was performed to optimize the proportion of enzymes produced by native strains of Trichoderma harzianum IOC 3844, Penicillium funiculosum ATCC 11797, and Aspergillus niger ATCC 1004, resulting in a proportion of 15, 50, and 35%, respectively. This mixture was able to hydrolyze 25 g/L of pretreated sugarcane bagasse with 91% of yield after 48 h of enzymatic reaction. Synergism along the hydrolysis process, besides the influence of lignin, hemicellulose, and solids loading, were also studied. Response surface methodology (RSM) based on Central Composite Rotatable Design was used to optimize solids and protein loadings to increase glucose release and enzymatic hydrolysis yield. The optimum solid and protein loadings established with RSM were 196 g/L and 24 mg/g cellulose, respectively, and under these conditions (94.1 ± 8) g/L of glucose were obtained, corresponding to a hydrolysis yield of 64%. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1222-1229, 2016.