RESUMO
The electron microscopic appearance of Chlamydia pneumoniae elementary bodies with pear-shaped, loose outer membrane has been suggested as one criterion of its classification as a new chlamydial species. The study of the original strain TW 183 in LCL 929 and HL cells and a low-passage isolate of Kajaani-6 isolate in HL cells revealed spherical compact elementary bodies common to other chlamydia.
Assuntos
Chlamydophila pneumoniae/ultraestrutura , Chlamydophila pneumoniae/crescimento & desenvolvimento , Microscopia EletrônicaRESUMO
During an epidemic of acute gastroenteritis in Helsinki, in March--May 1976, 18 out of 40 adult patients showed electron microscopic and/or serologic evidence for rotavirus infection. Rotavirus was most frequently seen in the fecal suspensions from 2 to 6 days after the onset of the symptoms but persisted in one patient for as long as 10 days. An increase in the complement-fixing (CF) serum antibody titers against the related Nebraska calf diarrhea virus (NCDV), or an initially high titer and subsequent significant decrease, was seen in all but one patient with rotavirus-positive feces, and in 6 additional patients. This suggests that using electron microscopy as the only diagnostic procedure a considerable number of rotavirus infections in adults remain undetected. Immune response against autologous or homologous rotavirus was also documented by immunoelectron microscopy. Complement-fixing antibody titers against NCDV decreased significantly from the convalescence values over a half-year observation period, but still remained clearly above the titers of a gastroenteritis-negative control population.
Assuntos
Surtos de Doenças , Gastroenterite/microbiologia , Vírus de RNA/isolamento & purificação , Rotavirus/isolamento & purificação , Viroses/microbiologia , Doença Aguda , Adulto , Anticorpos Antivirais/biossíntese , Testes de Fixação de Complemento , Fezes/microbiologia , Feminino , Finlândia , Humanos , Masculino , Pessoa de Meia-Idade , Rotavirus/imunologia , Rotavirus/ultraestruturaRESUMO
An epidemic of 44 cases of milker's nodules was recorded in the Tampere Central Hospital catchment area in Finland during the autumn of 1974. Exanthema or erythema multiforme-like secondary eruptions were seen in 10 cases. One female patient is reported in detail because of the simultaneous occurrence of erythema nodosum and erythema multiforme. The virological diagnosis was confirmed by electron microscopy.
