RESUMO
Acetylation of lysine 16 of histone H4 (H4K16ac) stands out among the histone modifications, because it decompacts the chromatin fiber. The metazoan acetyltransferase MOF (KAT8) regulates transcription through H4K16 acetylation. Antibody-based studies had yielded inconclusive results about the selectivity of MOF to acetylate the H4 N-terminus. We used targeted mass spectrometry to examine the activity of MOF in the male-specific lethal core (4-MSL) complex on nucleosome array substrates. This complex is part of the Dosage Compensation Complex (DCC) that activates X-chromosomal genes in male Drosophila. During short reaction times, MOF acetylated H4K16 efficiently and with excellent selectivity. Upon longer incubation, the enzyme progressively acetylated lysines 12, 8 and 5, leading to a mixture of oligo-acetylated H4. Mathematical modeling suggests that MOF recognizes and acetylates H4K16 with high selectivity, but remains substrate-bound and continues to acetylate more N-terminal H4 lysines in a processive manner. The 4-MSL complex lacks non-coding roX RNA, a critical component of the DCC. Remarkably, addition of RNA to the reaction non-specifically suppressed H4 oligo-acetylation in favor of specific H4K16 acetylation. Because RNA destabilizes the MSL-nucleosome interaction in vitro we speculate that RNA accelerates enzyme-substrate turn-over in vivo, thus limiting the processivity of MOF, thereby increasing specific H4K16 acetylation.
Assuntos
Proteínas de Drosophila , Histona Acetiltransferases , Código das Histonas , Animais , Masculino , Acetilação , Drosophila melanogaster/metabolismo , Drosophila melanogaster/genética , Proteínas de Drosophila/metabolismo , Proteínas de Drosophila/genética , Histona Acetiltransferases/metabolismo , Histona Acetiltransferases/genética , Histonas/metabolismo , Lisina/metabolismo , Proteínas Nucleares , Nucleossomos/metabolismo , Especificidade por Substrato , Fatores de Transcrição/metabolismo , Fatores de Transcrição/genéticaRESUMO
RNA unwinding by DExH-type helicases underlies most RNA metabolism and function. It remains unresolved if and how the basic unwinding reaction of helicases is regulated by auxiliary domains. We explored the interplay between the RecA and auxiliary domains of the RNA helicase maleless (MLE) from Drosophila using structural and functional studies. We discovered that MLE exists in a dsRNA-bound open conformation and that the auxiliary dsRBD2 domain aligns the substrate RNA with the accessible helicase tunnel. In an ATP-dependent manner, dsRBD2 associates with the helicase module, leading to tunnel closure around ssRNA. Furthermore, our structures provide a rationale for blunt-ended dsRNA unwinding and 3'-5' translocation by MLE. Structure-based MLE mutations confirm the functional relevance of our model for RNA unwinding. Our findings contribute to our understanding of the fundamental mechanics of auxiliary domains in DExH helicase MLE, which serves as a model for its human ortholog and potential therapeutic target, DHX9/RHA.
Assuntos
Proteínas de Drosophila , RNA Helicases , Animais , Humanos , Proteínas Cromossômicas não Histona/genética , DNA Helicases/genética , Drosophila/genética , Drosophila/metabolismo , Proteínas de Drosophila/metabolismo , Homeostase , RNA/metabolismo , RNA Helicases/metabolismo , RNA de Cadeia Dupla/genética , Fatores de Transcrição/metabolismoRESUMO
RNA-protein interactions are often mediated by dedicated canonical RNA binding domains. However, interactions through non-canonical domains with unknown specificity are increasingly observed, raising the question how RNA targets are recognized. Knowledge of the intrinsic RNA binding specificity contributes to the understanding of target selectivity and function of an individual protein. The presented in vitro RNA immunoprecipitation assay (vitRIP) uncovers intrinsic RNA binding specificities of isolated proteins using the total cellular RNA pool as a library. Total RNA extracted from cells or tissues is incubated with purified recombinant proteins, RNA-protein complexes are immunoprecipitated and bound transcripts are identified by deep sequencing or quantitative RT-PCR. Enriched RNA classes and the nucleotide frequency in these RNAs inform on the intrinsic specificity of the recombinant protein. The simple and versatile protocol can be adapted to other RNA binding proteins and total RNA libraries from any cell type or tissue. Graphic abstract: Figure 1. Schematic of the in vitro RNA immunoprecipitation (vitRIP) protocol.
RESUMO
The MLE DExH helicase and the roX lncRNAs are essential components of the chromatin modifying Dosage Compensation Complex (DCC) in Drosophila. To explore the mechanism of ribonucleoprotein complex assembly, we developed vitRIP, an unbiased, transcriptome-wide in vitro assay that reveals RNA binding specificity. We found that MLE has intrinsic specificity for U-/A-rich sequences and tandem stem-loop structures and binds many RNAs beyond roX in vitro. The selectivity of the helicase for physiological substrates is further enhanced by the core DCC. Unwinding of roX2 by MLE induces a highly selective RNA binding surface in the unstructured C-terminus of the MSL2 subunit and triggers-specific association of MLE and roX2 with the core DCC. The exquisite selectivity of roX2 incorporation into the DCC thus originates from intimate cooperation between the helicase and the core DCC involving two distinct RNA selection principles and their mutual refinement.
Assuntos
Montagem e Desmontagem da Cromatina , RNA Longo não Codificante/metabolismo , Transcriptoma , Animais , Proteínas Cromossômicas não Histona/genética , Proteínas Cromossômicas não Histona/metabolismo , Clonagem Molecular/métodos , DNA Helicases/genética , DNA Helicases/metabolismo , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Drosophila melanogaster , Ligação Proteica , RNA Longo não Codificante/genética , Proteínas de Ligação a RNA/genética , Proteínas de Ligação a RNA/metabolismo , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismoRESUMO
Maleless (MLE) is an evolutionary conserved member of the DExH family of helicases in Drosophila. Besides its function in RNA editing and presumably siRNA processing, MLE is best known for its role in remodelling non-coding roX RNA in the context of X chromosome dosage compensation in male flies. MLE and its human orthologue, DHX9 contain two tandem double-stranded RNA binding domains (dsRBDs) located at the N-terminal region. The two dsRBDs are essential for localization of MLE at the X-territory and it is presumed that this involves binding roX secondary structures. However, for dsRBD1 roX RNA binding has so far not been described. Here, we determined the solution NMR structure of dsRBD1 and dsRBD2 of MLE in tandem and investigated its role in double-stranded RNA (dsRNA) binding. Our NMR and SAXS data show that both dsRBDs act as independent structural modules in solution and are canonical, non-sequence-specific dsRBDs featuring non-canonical KKxAXK RNA binding motifs. NMR titrations combined with filter binding experiments and isothermal titration calorimetry (ITC) document the contribution of dsRBD1 to dsRNA binding in vitro. Curiously, dsRBD1 mutants in which dsRNA binding in vitro is strongly compromised do not affect roX2 RNA binding and MLE localization in cells. These data suggest alternative functions for dsRBD1 in vivo.
Assuntos
Proteínas Cromossômicas não Histona/química , DNA Helicases/química , Proteínas de Drosophila/química , Drosophila melanogaster/química , RNA Longo não Codificante/química , Fatores de Transcrição/química , Sequência de Aminoácidos , Animais , Sítios de Ligação , Proteínas Cromossômicas não Histona/genética , Proteínas Cromossômicas não Histona/metabolismo , Clonagem Molecular , DNA Helicases/genética , DNA Helicases/metabolismo , Mecanismo Genético de Compensação de Dose , Motivo de Ligação ao RNA de Cadeia Dupla , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/genética , Drosophila melanogaster/metabolismo , Escherichia coli/genética , Escherichia coli/metabolismo , Expressão Gênica , Regulação da Expressão Gênica , Vetores Genéticos/química , Vetores Genéticos/metabolismo , Cinética , Masculino , Modelos Moleculares , Conformação de Ácido Nucleico , Ligação Proteica , Conformação Proteica em alfa-Hélice , Conformação Proteica em Folha beta , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismoRESUMO
The MLE helicase remodels the roX lncRNAs, enabling the lncRNA-mediated assembly of the Drosophila dosage compensation complex. We identified a stable MLE core comprising the DExH helicase module and two auxiliary domains: a dsRBD and an OB-like fold. MLEcore is an unusual DExH helicase that can unwind blunt-ended RNA duplexes and has specificity for uridine nucleotides. We determined the 2.1 Å resolution structure of MLEcore bound to a U10 RNA and ADP-AlF4. The OB-like and dsRBD folds bind the DExH module and contribute to form the entrance of the helicase channel. Four uridine nucleotides engage in base-specific interactions, rationalizing the conservation of uridine-rich sequences in critical roX substrates. roX2 binding is orchestrated by MLE's auxiliary domains, which is prerequisite for MLE localization to the male X chromosome. The structure visualizes a transition-state mimic of the reaction and suggests how eukaryotic DEAH/RHA helicases couple ATP hydrolysis to RNA translocation.
Assuntos
Trifosfato de Adenosina/química , Proteínas Cromossômicas não Histona/química , DNA Helicases/química , Proteínas de Drosophila/química , RNA Helicases/química , RNA/química , Fatores de Transcrição/química , Trifosfato de Adenosina/genética , Trifosfato de Adenosina/metabolismo , Animais , Proteínas Cromossômicas não Histona/genética , Proteínas Cromossômicas não Histona/metabolismo , DNA Helicases/genética , DNA Helicases/metabolismo , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Drosophila melanogaster , Masculino , Estrutura Terciária de Proteína , RNA/genética , RNA/metabolismo , RNA Helicases/genética , RNA Helicases/metabolismo , Proteínas de Ligação a RNA/química , Proteínas de Ligação a RNA/genética , Proteínas de Ligação a RNA/metabolismo , Relação Estrutura-Atividade , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Cromossomo X/química , Cromossomo X/genética , Cromossomo X/metabolismoRESUMO
Directional transport of mRNA is a universal feature in eukaryotes, requiring the assembly of motor-dependent RNA-transport particles. The cytoplasmic transport of mRNAs is preceded by the nuclear assembly of pre-messenger ribonucleoprotein particles (mRNPs). In budding yeast, the asymmetric synthesis of HO 1 (ASH1) pre-mRNP originates already cotranscriptionally and passes through the nucleolus before its nuclear export. The nucleolar localization of ASH1 mRNA protein 1 (Loc1p) is required for efficient ASH1 mRNA localization. Immunoprecipitation experiments have revealed that Loc1p forms cocomplexes with other components of the ASH1 transport complex. However, it remains unclear how Loc1p is recruited into this mRNP and why Loc1p is important for ASH1 mRNA localization. Here we demonstrate that Loc1p undergoes a direct and specific interaction with the ASH1 mRNA-binding Swi5p-dependent HO expression protein 2 (She2p). This cocomplex shows higher affinity and specificity for RNA bearing localization elements than the individual proteins. It also stabilizes the otherwise transient binding of She2p to ASH1 mRNA, suggesting that cooperative mRNA binding of Loc1p with She2p is the required nuclear function of Loc1p for ASH1 mRNA localization. After nuclear export, myosin-bound She3p joins the ASH1 mRNP to form a highly specific cocomplex with She2p and ASH1 mRNA. Because Loc1p is found only in the nucleus, it must be removed from the complex directly before or after export. In vitro and in vivo experiments indicate that the synergistic interaction of She2p and She3p displaces Loc1p from the ASH1 complex, allowing free Loc1p to rapidly reenter the nucle(ol)us. Together these findings suggest an ordered process of nuclear assembly and reorganization for the maturation of localizing ASH1 mRNPs.
Assuntos
Proteínas Nucleares/metabolismo , Transporte de RNA/fisiologia , Proteínas de Ligação a RNA/metabolismo , Proteínas Repressoras/metabolismo , Ribonucleoproteínas/biossíntese , Proteínas de Saccharomyces cerevisiae/metabolismo , Ensaio de Desvio de Mobilidade Eletroforética , Escherichia coli , Proteínas Luminescentes , Ribonucleoproteínas/metabolismo , Saccharomycetales , Proteína Vermelha FluorescenteRESUMO
Dosage compensation in Drosophila involves a global activation of genes on the male X chromosome. The activating complex (MSL-DCC) consists of male-specific-lethal (MSL) proteins and two long, noncoding roX RNAs. The roX RNAs are essential for X-chromosomal targeting, but their contributions to MSL-DCC structure and function are enigmatic. Conceivably, the RNA helicase MLE, itself an MSL subunit, is actively involved in incorporating roX into functional DCC. We determined the secondary structure of roX2 and mapped specific interaction sites for MLE in vitro. Upon addition of ATP, MLE disrupted a functionally important stem loop in roX2. This RNA remodeling enhanced specific ATP-dependent association of MSL2, the core subunit of the MSL-DCC, providing a link between roX and MSL subunits. Probing the conformation of roX in vivo revealed a remodeled stem loop in chromatin-bound roX2. The active remodeling of a stable secondary structure by MLE may constitute a rate-limiting step for MSL-DCC assembly.
Assuntos
Trifosfato de Adenosina/farmacologia , Proteínas Cromossômicas não Histona/metabolismo , DNA Helicases/metabolismo , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , RNA Helicases/metabolismo , Proteínas de Ligação a RNA/genética , RNA/genética , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Cromossomo X/genética , Animais , Animais Geneticamente Modificados , Pareamento de Bases , Western Blotting , Cromatina/genética , Proteínas Cromossômicas não Histona/genética , DNA Helicases/genética , Proteínas de Drosophila/química , Drosophila melanogaster/genética , Drosophila melanogaster/crescimento & desenvolvimento , Drosophila melanogaster/metabolismo , Ensaio de Desvio de Mobilidade Eletroforética , Genes DCC/genética , Imunoprecipitação , Masculino , Mutação/genética , Conformação de Ácido Nucleico , RNA/química , RNA/metabolismo , RNA Helicases/genética , Proteínas de Ligação a RNA/química , Proteínas de Ligação a RNA/metabolismo , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Fatores de Transcrição/química , Transcrição Gênica , Cromossomo X/metabolismoRESUMO
The process of dosage compensation (DC) in Drosophila counterbalances the monosomy of the X chromosome in male flies by increasing the transcription from this unique chromosome in the two-fold range. Upon exclusive expression of male-specific lethal 2 (MSL2) in males, the dosage compensation machinery assembles on active X-chromosomal genes. Overexpression of MSL proteins leads to aberrant binding of complex components to autosomes. Accordingly, MSL levels have to be carefully regulated. Here we describe a new mechanism through which MSL2 can fulfill its role as the central regulator of the faithful biogenesis and functionality of the DC machinery. MSL2 is an E3 ligase that ubiquitylates itself and the other associated components when their stoichiometry is unbalanced, uncovering proteasome-dependent degradation as an additional layer of homeostatic control of MSL levels. Furthermore, systematic mapping of modification sites by mass spectrometry and chromatin interaction studies on the target protein MSL1 suggest that the role of MSL2-mediated ubiquitylation goes beyond proteolysis.
Assuntos
Proteínas de Ligação a DNA/metabolismo , Mecanismo Genético de Compensação de Dose , Proteínas de Drosophila/metabolismo , Homeostase , Proteínas Nucleares/metabolismo , Fatores de Transcrição/metabolismo , Animais , Células Cultivadas , Proteínas de Ligação a DNA/química , Proteínas de Ligação a DNA/genética , Proteínas de Drosophila/química , Proteínas de Drosophila/genética , Drosophila melanogaster/química , Drosophila melanogaster/citologia , Drosophila melanogaster/metabolismo , Masculino , Proteínas Nucleares/química , Proteínas Nucleares/genética , Fatores de Transcrição/química , Fatores de Transcrição/genéticaRESUMO
OBJECTIVE: To investigate psychological stress on the prognosis of the postoperative recurrence of keloids. METHODS: Patients with keloids (n=25), candidates for surgical resection and postoperative radiotherapy, had their psychological stress evaluated on the day before the surgical procedure. The parameters evaluated were pain and itching (Visual Numerical Scale), quality of life (Questionnaire QualiFibro/Cirurgia Plástica-UNIFESP), perceived stress (Perceived Stress Scale), depression and anxiety (Hospital Depression and Anxiety Scale), salivary cortisol and minimum and maximum galvanic skin responses (GSR) at rest and under stress (i.e., while the questionnaires were being filled out). Patients were evaluated during the 3rd, 6th, 9th and 12th months of postoperative care. During each return visit, two experts classified the lesions as non-recurrent and recurrent. RESULTS: The recurrence group presented the greatest values in GSR during a stressful situation. The chance of recurrence increased by 34% at each increase of 1000 arbitrary units in maximum GSR during stress. CONCLUSION: Psychological stress influenced the recurrence of keloids.
Assuntos
Queloide/psicologia , Queloide/cirurgia , Complicações Pós-Operatórias/psicologia , Estresse Psicológico/complicações , Adulto , Transtornos de Ansiedade/diagnóstico , Transtornos de Ansiedade/psicologia , Nível de Alerta/fisiologia , Transtorno Depressivo/diagnóstico , Transtorno Depressivo/psicologia , Feminino , Seguimentos , Resposta Galvânica da Pele/fisiologia , Humanos , Hidrocortisona/sangue , Masculino , Medição da Dor , Inventário de Personalidade/estatística & dados numéricos , Prognóstico , Psicometria , Qualidade de Vida/psicologia , Recidiva , Fatores de Risco , Saliva/química , Adulto JovemRESUMO
A large part of higher eukaryotic genomes is transcribed into RNAs lacking any significant open reading frame. This "non-coding part" has been shown to actively contribute to regulating gene expression, but the mechanisms are largely unknown. Particularly instructive examples are provided by the dosage compensation systems, which assure that the single X chromosome in male cells and the two X chromosomes in female cells give rise to similar amounts of gene product. Although this is achieved by very different strategies in mammals and fruit flies, long, non-coding RNAs (lncRNAs) are involved in both cases. Here we summarize recent progress towards unraveling the mechanisms, by which the Xist and roX RNAs mediate the selective association of regulators with individual target chromosomes, to initiate dosage compensation in mammals and fruit flies, respectively.
Assuntos
Cromossomos/genética , Mecanismo Genético de Compensação de Dose/genética , Regulação da Expressão Gênica/genética , RNA não Traduzido/química , RNA não Traduzido/genética , Transcrição Gênica/genética , Animais , Drosophila melanogaster/genética , HumanosRESUMO
In eukaryotes, hundreds of mRNAs are localized by specialized transport complexes. For localization, transcripts are recognized by RNA-binding proteins and incorporated into motor-containing messenger ribonucleoprotein particles (mRNPs). To date, the molecular assembly of such mRNPs is not well understood and most details on cargo specificity remain unresolved. We used ASH1-mRNA transport in yeast to provide a first assessment of where and how localizing mRNAs are specifically recognized and incorporated into mRNPs. By using in vitro-interaction and reconstitution assays, we found that none of the implicated mRNA-binding proteins showed highly specific cargo binding. Instead, we identified the cytoplasmic myosin adapter She3p as additional RNA-binding protein. We further found that only the complex of the RNA-binding proteins She2p and She3p achieves synergistic cargo binding, with an at least 60-fold higher affinity for localizing mRNAs when compared to control RNA. Mutational studies identified a C-terminal RNA-binding fragment of She3p to be important for synergistic RNA binding with She2p. The observed cargo specificity of the ternary complex is considerably higher than previously reported for localizing mRNAs. It suggests that RNA binding for mRNP localization generally exhibits higher selectivity than inferred from previous in vitro data. This conclusion is fully consistent with a large body of in vivo evidence from different organisms. Since the ternary yeast complex only assembles in the cytoplasm, specific mRNA recognition might be limited to the very last steps of mRNP assembly. Remarkably, the mRNA itself triggers the assembly of mature, motor-containing complexes. Our reconstitution of a major portion of the mRNA-transport complex offers new and unexpected insights into the molecular assembly of specific, localization-competent mRNPs and provides an important step forward in our mechanistic understanding of mRNA localization in general.
Assuntos
Citoplasma/metabolismo , RNA Mensageiro/metabolismo , Proteínas de Ligação a RNA/metabolismo , Ribonucleoproteínas/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Saccharomyces cerevisiae/metabolismo , Sequência de Aminoácidos , Núcleo Celular/metabolismo , Sequências Repetidas Invertidas , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Conformação de Ácido Nucleico , Ligação Proteica , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína , Transporte Proteico , Transporte de RNA , Proteínas de Ligação a RNA/genética , Proteínas Repressoras/genética , Proteínas Repressoras/metabolismo , Proteínas de Saccharomyces cerevisiae/genéticaRESUMO
In eukaryotic cells, dozens to hundreds of different mRNAs are localized by specialized motor-dependent transport complexes. One of the best-studied examples for directional mRNA transport is the localization of ASH1 mRNA in Saccharomyces cerevisiae. For transport, ASH1 mRNA is bound by the unusual RNA-binding protein She2p. Although previous results indicated that She2p forms dimers required for RNA binding and transcript localization, it remained unclear if the dimer constitutes the minimal RNA-binding unit assembling in vivo. By using analytical ultracentrifugation we found that She2p forms larger oligomeric complexes in solution. We also identified a point mutant that shows impaired oligomer formation. Size-exclusion chromatography suggests that She2p forms defined tetramers at physiological concentrations. Subsequent structural studies by small-angle X-ray scattering confirmed this finding and demonstrated that the previously observed She2p dimers interact in a head-to-head conformation to form an elongated tetrameric complex. This She2p tetramer suggests the generation of large continuous RNA-binding surfaces at both sides of the complex. Biochemical studies and immunostaining of cells confirmed that She2p tetramer formation is required for RNA binding, efficient mRNP assembly, and mRNA localization in vivo. Our finding on She2p tetramerization resolves previously raised questions on complex formation and mRNP function.
Assuntos
Multimerização Proteica , RNA Mensageiro/metabolismo , Proteínas de Ligação a RNA/química , Proteínas de Ligação a RNA/metabolismo , Ribonucleoproteínas/metabolismo , Proteínas de Saccharomyces cerevisiae/química , Proteínas de Saccharomyces cerevisiae/metabolismo , Saccharomyces cerevisiae/metabolismo , Cristalografia por Raios X , Modelos Moleculares , Mutação , Ligação Proteica , Estrutura Quaternária de Proteína , Proteínas de Ligação a RNA/genética , Proteínas de Saccharomyces cerevisiae/genética , UltracentrifugaçãoRESUMO
BACKGROUND: The visible aspect of skin lesions and its psychological impact interfere in the quality of life of patients. OBJECTIVES: To assess the quality of life and site of dermatological lesion; to check associations between variables and compare levels of quality of life in patients with lesions on the face and/or hands and patients with lesions in parts of the body other than face and/or hands. METHODS: descriptive, association-based cross-sectional study. Two hundred and five subjects were assessed using SF-36 Generic Life Quality Questionnaire "The Medical Outcomes Study 36-item Short-Form Health Survey" (SF-36); the Dermatology Life Quality Index DLQI-BRA, and socio-demographical and lesion site data files. RESULTS: No significant differences were observed in the results for quality of life between the two groups but the number of associations between SF-36 and DLQI-BRA was higher in the group with lesions on the face and/or hands. Significant differences were verified in a further detailed division into five groups. The significant difference (p=0.05) appeared between the group with lesions on the face and/or hands and the group with generalized lesions, being that the latter presented an average ranking of 114.06 compared with 69.1935 in the former group. CONCLUSION: Regardless of the site of lesion, the feeling of exposure and the damages to which the dermatological patients are exposed are similar. It seems that skin diseases bring about the feeling of exposure and embarrassment no matter which site of the body is affected because in any intimate approach there is some sort of exposure involved.
Assuntos
Inquéritos Epidemiológicos , Qualidade de Vida/psicologia , Dermatopatias/patologia , Dermatopatias/psicologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Estudos Transversais , Dermatite Atópica/patologia , Dermatite Atópica/psicologia , Feminino , Humanos , Ceratose/patologia , Ceratose/psicologia , Masculino , Pessoa de Meia-Idade , Psoríase/patologia , Psoríase/psicologia , Índice de Gravidade de Doença , Estatísticas não Paramétricas , Inquéritos e Questionários , Adulto JovemRESUMO
FUNDAMENTOS - O aspecto visível das lesões de pele e seu impacto psicológico interferem na qualidade de vida dos pacientes. OBJETIVOS - Avaliar qualidade de vida e localização da lesão dermatológica, verificar associação entre variáveis e comparar níveis de qualidade de vida em pacientes com lesões na face/ou mãos e pacientes somente com lesões em outras regiões do corpo que não face e/ou mãos. MÉTODO - Estudo transversal, descritivo, de ssociação. Foram avaliados 205 sujeitos através do Questionário Genérico de Qualidade de Vida- SF-36 "The Medical Outcomes Study 36-item Short-Form Health Survey" (SF-36) e do Dermatology Life quality Index, fichas de dados sócio-demográficos e de localização da lesão. RESULTADOS - Não foram observadas diferenças significativas na qualidade de vida entre os dois grupos, mas o número de associações entre SF-36 e DLQI-BRA foi superior no grupo com lesões em face e/ou mãos. Diferenças significativas foram verificadas quando da divisão em cinco grupos. A diferença significativa (p=0,05) foi entre o grupo com lesões somente em face e/ou mãos e o grupo com lesões generalizadas, sendo que este último teve posto médio de 114,06 contra 69,1935 do outro grupo. CONCLUSÕES - Independente da localização da lesão, o sentimento de exposição e os prejuízos a que fica sujeito o paciente dermatológico são semelhantes. As doenças da pele, ao que parece, provocam sentimento de exposição e constrangimento, independente do local do corpo acometido, pois na aproximação mais íntima de outra pessoa está implicada certa exposição.
BACKGROUND - The visible aspect of skin lesions and its psychological impact interfere in the quality of life of patients. OBJECTIVES- To assess the quality of life and site of dermatological lesion; to check associations between variables and compare levels of quality of life in patients with lesions on the face and/or hands and patients with lesions in parts of the body other than face and/or hands. METHODS - descriptive, association-based cross-sectional study. Two hundred and five subjects were assessed using SF-36 Generic Life Quality Questionnaire "The Medical Outcomes Study 36-item Short-Form Health Survey" (SF-36); the Dermatology Life Quality Index DLQI-BRA, and socio-demographical and lesion site data files. RESULTS - No significant differences were observed in the results for quality of life between the two groups but the number of associations between SF-36 and DLQI-BRA was higher in the group with lesions on the face and/or hands. Significant differences were verified in a further detailed division into five groups. The significant difference (p=0.05) appeared between the group with lesions on the face and/or hands and the group with generalized lesions, being that the latter presented an average ranking of 114.06 compared with 69.1935 in the former group. CONCLUSION - Regardless of the site of lesion, the feeling of exposure and the damages to which the dermatological patients are exposed are similar. It seems that skin diseases bring about the feeling of exposure and embarrassment no matter which site of the body is affected because in any intimate approach there is some sort of exposure involved.
Assuntos
Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Inquéritos Epidemiológicos , Qualidade de Vida/psicologia , Dermatopatias/patologia , Dermatopatias/psicologia , Estudos Transversais , Dermatite Atópica/patologia , Dermatite Atópica/psicologia , Ceratose/patologia , Ceratose/psicologia , Psoríase/patologia , Psoríase/psicologia , Índice de Gravidade de Doença , Estatísticas não Paramétricas , Inquéritos e Questionários , Adulto JovemRESUMO
O presente trabalho apresenta uma revisão bibliométrica da produção brasileira na interface psicologia espiritualidade-religiosidade, no período de 1998 a 2006. Para isso definimos temas (psicologia e espiritualidade/religiosidade) e termos de buscas ("religiosidade" e "psicologia", "espiritualidade" e "psicologia", e "espiritualidade" e "religiosidade") realizadas em bases de dados de artigos e periódicos científicos on-line, de acesso gratuito e privado, além da busca manual, realizada nas bibliotecas da Pontifícia Universidade Católica do Rio Grande do Sul, PUC-RS e da Universidade Católica de Pelotas, UCPel. Foram priorizados periódicos classificados como QUALIS Nacional A, B e C, e os Locais. Os resultados apontam que até 2006 os dados da produção brasileira na interface psicologia espiritualidade/religiosidade menores em relação ao total da produção. No entanto, esse número cresce a partir do referido período. Assim, consideramos o panorama da produção como crescente, uma informação bastante relevante, em função do importante papel da dimensão espiritual-religiosa no desenvolvimento pessoal.
A bibliometric revision of the Brazilian production about the interface between spirituality and psychology were analyzed, from 1998 to 2006. To achieve that were defined the themes (psychology and spirituality-religiosity) and search terms ("religiosity and psychology", "spirituality and psychology", and "spirituality" and "religiosity ") and searches were done in article and scientifical periodic online databases, of free and private access. Beyond that, manual searches were made at the libraries of the Pontifícia Universidade Católica of Rio Grande do Sul and Universidade Católica de Pelotas,. Were prioritized the periodic classified as National QUALIS A, B and C, and the Local ones. The results point out in the direction that until 2006, data on Brazilian production about the interface between spirituality and psychology are very few related to production totals. However, this number grows larger after the referred period. Thus, the production scenario were consider as growing, a very relevant information, in face of the important role of the spiritual-religious dimension in the personal development.
Assuntos
Bibliometria , Publicação Periódica , Religião e Psicologia , EspiritualidadeRESUMO
O presente trabalho apresenta uma revisão bibliométrica da produção brasileira na interface psicologia espiritualidade-religiosidade, no período de 1998 a 2006. Para isso definimos temas (psicologia e espiritualidade/religiosidade) e termos de buscas ('religiosidade' e 'psicologia', 'espiritualidade' e 'psicologia', e 'espiritualidade' e 'religiosidade') realizadas em bases de dados de artigos e periódicos científicos on-line, de acesso gratuito e privado, além da busca manual, realizada nas bibliotecas da Pontifícia Universidade Católica do Rio Grande do Sul, PUC-RS e da Universidade Católica de Pelotas, UCPel. Foram priorizados periódicos classificados como QUALIS Nacional A, B e C, e os Locais. Os resultados apontam que até 2006 os dados da produção brasileira na interface psicologia espiritualidade/religiosidade menores em relação ao total da produção. No entanto, esse número cresce a partir do referido período. Assim, consideramos o panorama da produção como crescente, uma informação bastante relevante, em função do importante papel da dimensão espiritual-religiosa no desenvolvimento pessoal(AU)
A bibliometric revision of the Brazilian production about the interface between spirituality and psychology were analyzed, from 1998 to 2006. To achieve that were defined the themes (psychology and spirituality-religiosity) and search terms ('religiosity and psychology', 'spirituality and psychology', and 'spirituality' and 'religiosity ') and searches were done in article and scientifical periodic online databases, of free and private access. Beyond that, manual searches were made at the libraries of the Pontifícia Universidade Católica of Rio Grande do Sul and Universidade Católica de Pelotas,. Were prioritized the periodic classified as National QUALIS A, B and C, and the Local ones. The results point out in the direction that until 2006, data on Brazilian production about the interface between spirituality and psychology are very few related to production totals. However, this number grows larger after the referred period. Thus, the production scenario were consider as growing, a very relevant information, in face of the important role of the spiritual-religious dimension in the personal development(AU)
Assuntos
Bibliometria , Religião e Psicologia , Espiritualidade , Publicação PeriódicaRESUMO
Este trabalho investigou a freqüência da depressão em indivíduos cardiopatas, com e sem diagnóstico de infarto agudo do miocárdio (IAM), e indivíduos sem diagnóstico de cardiopatia. Trata-se de estudo prospectivo com 168 pacientes divididos em três grupos: 60 internados (diagnóstico de IAM), 49 ambulatoriais (diagnóstico de doença cardiovascular sem infarto) e 59 da população em geral (sem diagnóstico de doença cardiovascular), de ambos os sexos, entre 35 a 65 anos, investigados por meio do Inventário de Depressão de Beck (BDI), do Inventário de Ansiedade de Beck (BAI) e do Teste de Qualidade de Vida (WHOQOL). A prevalência de depressão no grupo IAM foi 48,3 por cento. As variáveis significativas associadas à depressão foram: história familiar (RC=2,82 - IC95 por cento=1,12;7,08 - p=0,028), domínio psicológico do WHOQOL (RC=0,93 - IC95 por cento=0,89;0,98 - p=0,006) e escore de ansiedade (RC=1,08 - IC95 por cento=1,02;1,14 - p=0,012). Os resultados sugerem que os transtornos de depressão não são desencadeados pelo IAM, mas que estão presentes antes da admissão hospitalar, destacando a importância do rastreamento dos pacientes portadores de doença coronariana crônica.(AU)
This study investigated the frequency of depression in individuals with cardiovascular disease, with or without an acute myocardial infarction (AMI) diagnosis, and individuals with no cardiovascular disease. It is a prospective study with 168 patients of both genders, from 35 to 65 years old, divided into three groups: 60 inpatients (diagnosed with AMI), 49 outpatients (diagnosed with cardiovascular disease without infarction), and 59 of the general population (without diagnosis of cardiovascular disease). The groups were investigated by applying questionnaires, which included the Beck Depression Inventory (BDI), the Beck Anxiety Inventory (BAI) and the Quality of Life Test (WHOQOL). The prevalence of depression in the AMI group was 48.3 percent. The variables that remained significantly associated to depression were: family history (OR=2.82 - 95 percentCI=1.12;7.08 - p=0.028), WHOQOL psychological domain (OR=0.93 - 95 percentCI=0.89;0.98 - p=0.006), and anxiety score (OR=1.08 - 95 percentCI=1.02;1.14 - p=0.012). These results suggest that depression disorders are not triggered post-AMI but can be present before admission to the hospital, which shows the need to investigate people with chronic coronary disease.(AU)
Assuntos
Infarto do Miocárdio , Qualidade de Vida , Depressão , AnsiedadeRESUMO
Este trabalho investigou a freqüência da depressão em indivíduos cardiopatas, com e sem diagnóstico de infarto agudo do miocárdio (IAM), e indivíduos sem diagnóstico de cardiopatia. Trata-se de estudo prospectivo com 168 pacientes divididos em três grupos: 60 internados (diagnóstico de IAM), 49 ambulatoriais (diagnóstico de doença cardiovascular sem infarto) e 59 da população em geral (sem diagnóstico de doença cardiovascular), de ambos os sexos, entre 35 a 65 anos, investigados por meio do Inventário de Depressão de Beck (BDI), do Inventário de Ansiedade de Beck (BAI) e do Teste de Qualidade de Vida (WHOQOL). A prevalência de depressão no grupo IAM foi 48,3 por cento. As variáveis significativas associadas à depressão foram: história familiar (RC=2,82 - IC95 por cento=1,12;7,08 - p=0,028), domínio psicológico do WHOQOL (RC=0,93 - IC95 por cento=0,89;0,98 - p=0,006) e escore de ansiedade (RC=1,08 - IC95 por cento=1,02;1,14 - p=0,012). Os resultados sugerem que os transtornos de depressão não são desencadeados pelo IAM, mas que estão presentes antes da admissão hospitalar, destacando a importância do rastreamento dos pacientes portadores de doença coronariana crônica.
This study investigated the frequency of depression in individuals with cardiovascular disease, with or without an acute myocardial infarction (AMI) diagnosis, and individuals with no cardiovascular disease. It is a prospective study with 168 patients of both genders, from 35 to 65 years old, divided into three groups: 60 inpatients (diagnosed with AMI), 49 outpatients (diagnosed with cardiovascular disease without infarction), and 59 of the general population (without diagnosis of cardiovascular disease). The groups were investigated by applying questionnaires, which included the Beck Depression Inventory (BDI), the Beck Anxiety Inventory (BAI) and the Quality of Life Test (WHOQOL). The prevalence of depression in the AMI group was 48.3 percent. The variables that remained significantly associated to depression were: family history (OR=2.82 - 95 percentCI=1.12;7.08 - p=0.028), WHOQOL psychological domain (OR=0.93 - 95 percentCI=0.89;0.98 - p=0.006), and anxiety score (OR=1.08 - 95 percentCI=1.02;1.14 - p=0.012). These results suggest that depression disorders are not triggered post-AMI but can be present before admission to the hospital, which shows the need to investigate people with chronic coronary disease.
Assuntos
Infarto do Miocárdio , Qualidade de Vida , Ansiedade , DepressãoRESUMO
Este estudo teve o objetivo de avaliar a qualidade de vida e bem-estar espiritual em pessoas vivendo com HIV/Aids. A amostra foi constituída de 200 pessoas vivendo com HIV/Aids (111 mulheres e 89 homens), de 19 a 67 anos, 143 sintomáticos e 57 assintomáticos. Do total, 62,5 por cento faziam uso de terapia anti-retroviral. Os resultados apontam para a correlação significativa entre os domínios da qualidade de vida (WHOQOL-HIV bref) e bem-estar espiritual (SWBS). O domínio da qualidade de vida, espiritualidade, religiosidade e crenças pessoais e a subescala bem-estar religioso apresentaram os escores mais altos. Obtiveram-se correlações altamente significativas entre os domínios da qualidade de vida física, relações sociais e espiritualidade, religiosidade e crenças pessoais, e os do bem-estar religioso, bem-estar existencial e bem-estar espiritual no grupo sintomático/Aids. Discute-se a importância das variáveis religiosidade e espiritualidade no processo de resiliência e de proteção à saúde. A importância da inserção dos fatores de proteção à saúde necessita ser destacada nas pesquisas, nas intervenções do psicólogo e no planejamento de políticas públicas de saúde.(AU)
Current study evaluates the quality of life and spiritual well-being in HIV/Aids people. Two hundred HIV individuals were taken as samples (111 women and 89 men), aged between 19 to 67 years old, 143 symptomatic/Aids and 57 asymptomatic, 62.5 percent had taken anti-retroviral therapy. Results point to a significant correlation between the quality of life (WHOQOL-HIV bref) and spiritual well being (SWB). The spiritual, religiosity and personal beliefs and the subscale religious well being had the highest scores. Significant correlations were obtained in symptomatic/Aids group between quality of life concerning physical, social relationship and spiritual, religiosity and personal beliefs with the religious well-being, the existential well being and the spiritual well-being. The importance of the variables religiosity and spirituality within the resilience and health protection process was also discussed. Discussing health protection in research, in psychologist's interventions and in health public policies should be enhanced.(AU)
Este estudio tuvo el objetivo de evaluar la cualidad de vida y bienestar espiritual de personas viviendo con VIH/SIDA. La muestra fue de 200 pacientes viviendo con VIH/SIDA (111 mujeres y 89 hombres), de 19 a 67 años, 143 sintomáticos y 57 asintomáticos, del total, 62,5 por ciento hacían uso de terapia antirretroviral. Los resultados apuntan hacia la correlación significativa entre los dominios de la cualidad de vida (WHOQOL-HIV bref) y bienestar espiritual (SWBS). El dominio de la cualidad de vida, espiritualidad, religiosidad y creencias personales como la subescala bienestar religioso presentaron los escores más altos. Se obtuvieron correlaciones altamente significativas entre los dominios de cualidad de vida físico, relaciones sociales y espiritualidad, religiosidad y creencias personales con el bienestar religioso, existencial y espiritual del grupo sintomático/SIDA. Se discute la importancia de las variables religiosidad y espiritualidad en el proceso de resiliencia y de protección a la salud. La importancia de la inserción de los factores de protección a la salud necesita ser destacada en las investigaciones, en las intervenciones del psicólogo y en el planeamiento de políticas públicas de salud.(AU)
