Longitudinal genetic dynamics of weaning index and implications for cow-calf production efficiency.

In beef cattle, the selection for higher weights at young ages has been questioned with the argument that this criterion may increase the adult weight of cows, resulting in higher costs. Therefore, selection criteria should be employed to increase weights at young ages with minimal impact on the adult weight of cows. Additionally, the relationship between measures of cow production efficiency and other well-established selection criteria in breeding programs remains poorly understood. The objective of this study was to longitudinally evaluate the relationship between the weaning index (WIndex) as a measure of efficiency and growth traits of the cows. Possible changes over time in WIndex due to selection applied for yearling weight (YW) were also investigated. The WIndex was proposed to maximize genetic response in the weaning weight of the calf while maintaining genetic gain in BW of the cow at zero. A random regression model was adopted to estimate correlations between WIndex, BW, hip height (HH), and body condition score (BCS) using records of Nelore cows from three lines. Genetic trends were calculated for the control line (NeC) and lines selected for greater YW (NeS and NeT). The age of 3 years was the most critical for the weaning efficiency of the cows. At this stage, young cows are still growing and wean lighter calves than their adult counterparts. The genetic correlation estimates between WIndex and BW (-0.58 to 0.04), HH (-0.05 to -0.34), and BCS (-0.51 to -0.17) were close to zero or negative. BW and HH were strongly correlated genetically across all ages (0.73-0.76). In general, HH exhibited a weak and negative genetic relationship with BCS. The genetic correlation between BW and BCS was stronger for advanced ages (0.45-0.68). In lines selected for YW, important increases in WIndex were observed. However, NeS has been selected since the 1980s until the present for YW, and thus, it showed a more pronounced trend of increasing BW and, consequently, a more modest trend of increasing WIndex compared to NeT. In contrast, WIndex exhibited a trend close to zero for NeC. In this context, monitoring HH and BCS can be useful to avoid losses in the weaning efficiency of cows. Furthermore, we suggest that one way to mitigate efficiency losses in calf production could involve stabilizing the BW of cows and increasing the weaning weight of calves using the WIndex.

Effect of follicular wave stage on potential fertility predictors and their repeatability coefficient in prepubertal Bos indicus (Nellore) and Bos taurus (Caracu) heifers.

In an attempt to develop accessible methods for the precocious individual selection based on phenotypic attributes related to reproductive superiority, this study evaluated the effects of follicular wave stages (emergence and dominance) and the repeatability coefficients of potential fertility predictors (vulvar morphometry, uterine biometry, echogenicity and echotexture, ovarian biometry, and antral follicle count - AFC) in two breeds of purebred prepubertal heifers. Nellore (n = 30) and Caracu (n = 28) heifers were submitted to a sequence of 11 evaluations conducted every 48 h (D0 - random day of the antral follicular wave until D20) to study potential fertility indicators under natural conditions. The data obtained were compared according to breed and follicular wave stage. Statistical analysis included the fixed effects of breed, evaluation day, and their statistical interaction and was performed using MIXED, GENMOD, GLM, and CORR procedures of the SAS program. Breed was found to influence rima height, ovarian area, and AFC, which were greater in Nellore heifers (P < 0.02). The follicular wave stage also influenced most of the potential predictors, highlighting AFC which was higher in the presence of a dominant follicle in both breeds (P < 0.0001). The repeatability coefficients for vulvar width (0.76 and 0.66), ovarian area (0.70 and 0.62), and AFC (0.76 and 0.74) were considered to be high in Nellore and Caracu heifers, respectively. Only ovarian biometry was able to predict AFC (Pearson correlation coefficient ≥ 0.66; P < 0.0001) in prepubertal heifers. The results indicate that most of the phenotypic reproductive parameters analyzed can be characterized throughout prepuberty using a single measure since they are intrinsic attributes of the individual.

In vivo ultrasound and biometric measurements predict the empty body chemical composition in Nellore cattle.

Evaluation of the body chemical composition of beef cattle can only be measured postmortem and those data cannot be used in real production scenarios to adjust nutritional plans. The objective of this study was to develop multiple linear regression equations from in vivo measurements, such as ultrasound parameters [backfat thickness (uBFT, mm), rump fat thickness (uRF, mm), and ribeye area (uLMA, cm2)], shrunk body weight (SBW, kg), age (AG, d), hip height (HH, m), as well as from postmortem measurements (composition of the 9th to 11th rib section) to predict the empty body and carcass chemical composition for Nellore cattle. Thirty-three young bulls were used (339 ± 36.15 kg and 448 ± 17.78 d for initial weight and age, respectively). Empty body chemical composition (protein, fat, water, and ash in kg) was obtained by combining noncarcass and carcass components. Data were analyzed using the PROC REG procedure of SAS software. Mallows' Cp values were close to the ideal value of number of independent variables in the prediction equations plus one. Equations to predict chemical components of both empty body and carcass using in vivo measurements presented higher R2 values than those determined by postmortem measurements. Chemical composition of the empty body using in vivo measurements was predicted with R2 > 0.73. Equations to predict chemical composition of the carcass from in vivo measurements showed R2 lower (R2< 0.68) than observed for empty body, except for the water (R2 = 0.84). The independent variables SBW, uRF, and AG were sufficient to predict the fat, water, energy components of the empty body, whereas for estimation of protein content the uRF, HH, and SBW were satisfactory. For the calculation of the ash, the SBW variable in the equation was sufficient. Chemical compounds from components of the empty body of Nellore cattle can be calculated by the following equations: protein (kg) = 47.92 + 0.18 × SBW - 1.46 × uRF - 30.72 × HH (R2 = 0.94, RMSPE = 1.79); fat (kg) = 11.33 + 0.16 × SBW + 2.09 × uRF - 0.06 × AG (R2 = 0.74, RMSPE = 4.18); water (kg) = - 34.00 + 0.55 × SBW + 0.10 × AG - 2.34 × uRF (R2 = 0.96, RMSPE = 5.47). In conclusion, the coefficients of determination (for determining the chemical composition of the empty body) of the equations derived from in vivo measures were higher than those of the equations obtained from rib section measurements taken postmortem, and better than coefficients of determination of the equations to predict the chemical composition of the carcass.

Avaliação de intervalos de tempo para registro do comportamento ingestivo de bovinos confinados individualmente / Evaluation of recording intervals to estimate the ingestive behavior of cattle housed in individual pens

O objetivo desse trabalho foi comparar o intervalo de tempo de registro de cinco minutos com quatro intervalos de tempo (10, 15, 20 e 30 minutos), para avaliação dos comportamentos comendo, ruminando, em ócio, em pé, apresentados por bovinos confinados individualmente. Foram utilizados 62 machos Nelore desmamados, confinados durante 112 dias. O comportamento foi registrado a cada 28 dias, durante 24h, por observações diretas com rota de amostragem scan e intervalos de registro de 5 minutos, para o cálculo das seguintes características: tempo de alimentação, tempo de ruminação, tempo em ócio, tempo em pé e frequência de alimentação. Foram comparados os intervalos de registro por meio de variâncias, correlações e médias das características. Observou-se para as características, tendência de aumento da variância residual à medida que o intervalo de tempo entre dois registros aumentou, com exceção da frequência alimentar, em que foi observada tendência de diminuição da variância residual com o aumento do intervalo de registro. As médias ajustadas para as medidas de comportamento ingestivo obtidas nos intervalos de registro de 5, 10, 15, 20 e 30 minutos não diferiram significativamente (P>0,05) entre si, com exceção de tempo de alimentação, que apresentou menor valor (P<0,05) no intervalo de registro de 30 minutos em comparação ao intervalo de 5 minutos. Observou-se que até o intervalo de registro de 15 minutos, as correlações com o intervalo de registro de 5 minutos podem ser consideradas altas. Entretanto, acima de 15 minutos de intervalo de registro, a correlação com o intervalo de registro de 5 minutos cai abruptamente, deixando claro que intervalos de registros superiores a 15 minutos são inadequados para estimar as características de comportamento ingestivo de bovinos avaliadas. (AU)

: The objective of this study was to compare five-minute recording intervals with four time intervals (10, 15, 20 and 30 minutes) for estimation of eating, ruminating, idling and standing time, as well as meal frequency, of cattle housed in individual pens. Sixty-two weaned Nellore males housed in individual pens for 112 days were used. Ingestive behavior was evaluated every 28 days for 24 h. Direct observations were made using scan sampling and instantaneous sampling at intervals of 5 minutes. The recording intervals were evaluated using error variance, correlations, and adjusted means. For all traits, the residual variance tended to increase with increasing recording interval, except for meal frequency whose residual variance decreased with increasing recording interva. The adjusted means for the ingestive behavior measurements obtained at intervals of 5, 10, 15, 20 and 30 minutes did not differ significantly (P>0.05), except for meal frequency which was lower (P<0.05) in the 30-minute recording interval. The correlations with the 5-minute recording interval were considered high up to the 15-minute interval. However, for longer recording intervals, the correlation with the 5-minute recording interval decreased abruptly, showing that recording intervals longer than 15 minutes are inadequate to estimate ingestive behavior traits of cattle.(AU)

Avaliação de intervalos de tempo para registro do comportamento ingestivo de bovinos confinados individualmente / Evaluation of recording intervals to estimate the ingestive behavior of cattle housed in individual pens

O objetivo desse trabalho foi comparar o intervalo de tempo de registro de cinco minutos com quatro intervalos de tempo (10, 15, 20 e 30 minutos), para avaliação dos comportamentos comendo, ruminando, em ócio, em pé, apresentados por bovinos confinados individualmente. Foram utilizados 62 machos Nelore desmamados, confinados durante 112 dias. O comportamento foi registrado a cada 28 dias, durante 24h, por observações diretas com rota de amostragem scan e intervalos de registro de 5 minutos, para o cálculo das seguintes características: tempo de alimentação, tempo de ruminação, tempo em ócio, tempo em pé e frequência de alimentação. Foram comparados os intervalos de registro por meio de variâncias, correlações e médias das características. Observou-se para as características, tendência de aumento da variância residual à medida que o intervalo de tempo entre dois registros aumentou, com exceção da frequência alimentar, em que foi observada tendência de diminuição da variância residual com o aumento do intervalo de registro. As médias ajustadas para as medidas de comportamento ingestivo obtidas nos intervalos de registro de 5, 10, 15, 20 e 30 minutos não diferiram significativamente (P>0,05) entre si, com exceção de tempo de alimentação, que apresentou menor valor (P<0,05) no intervalo de registro de 30 minutos em comparação ao intervalo de 5 minutos. Observou-se que até o intervalo de registro de 15 minutos, as correlações com o intervalo de registro de 5 minutos podem ser consideradas altas. Entretanto, acima de 15 minutos de intervalo de registro, a correlação com o intervalo de registro de 5 minutos cai abruptamente, deixando claro que intervalos de registros superiores a 15 minutos são inadequados para estimar as características de comportamento ingestivo de bovinos avaliadas.

: The objective of this study was to compare five-minute recording intervals with four time intervals (10, 15, 20 and 30 minutes) for estimation of eating, ruminating, idling and standing time, as well as meal frequency, of cattle housed in individual pens. Sixty-two weaned Nellore males housed in individual pens for 112 days were used. Ingestive behavior was evaluated every 28 days for 24 h. Direct observations were made using scan sampling and instantaneous sampling at intervals of 5 minutes. The recording intervals were evaluated using error variance, correlations, and adjusted means. For all traits, the residual variance tended to increase with increasing recording interval, except for meal frequency whose residual variance decreased with increasing recording interva. The adjusted means for the ingestive behavior measurements obtained at intervals of 5, 10, 15, 20 and 30 minutes did not differ significantly (P>0.05), except for meal frequency which was lower (P<0.05) in the 30-minute recording interval. The correlations with the 5-minute recording interval were considered high up to the 15-minute interval. However, for longer recording intervals, the correlation with the 5-minute recording interval decreased abruptly, showing that recording intervals longer than 15 minutes are inadequate to estimate ingestive behavior traits of cattle.

Residual feed intake, carcass traits and meat quality in Nellore cattle.

A total of 127 Nellore bulls classified as low and high residual feed intake (RFI), from three selection lines, were evaluated in five experiments aiming to identify associations between RFI, carcass, and meat quality traits. Feedlot performance, carcass traits, and meat quality of Longissimus muscle were evaluated. A mixed model including the fixed effects of RFI class, selection line, interaction between RFI class and selection line, and age at slaughter as a covariate, and the random effects of diet within experiment and experiment was used, with means compared by the t-test. Feed intake average difference was 0.962kg/day; low RFI animals consumed 0.479kg/day less feed and high RFI animals consumed 0.483kg/day more feed than expected. No differences between RFI classes were detected for most of studied variables, demonstrating the absence of unfavorable associations between RFI and carcass and meat quality traits. Low RFI Nellore animals use feed efficiently and produce good quality carcasses and meat.

Accuracies of genomic prediction of feed efficiency traits using different prediction and validation methods in an experimental Nelore cattle population.

Animal feeding is the most important economic component of beef production systems. Selection for feed efficiency has not been effective mainly due to difficult and high costs to obtain the phenotypes. The application of genomic selection using SNP can decrease the cost of animal evaluation as well as the generation interval. The objective of this study was to compare methods for genomic evaluation of feed efficiency traits using different cross-validation layouts in an experimental beef cattle population genotyped for a high-density SNP panel (BovineHD BeadChip assay 700k, Illumina Inc., San Diego, CA). After quality control, a total of 437,197 SNP genotypes were available for 761 Nelore animals from the Institute of Animal Science, Sertãozinho, São Paulo, Brazil. The studied traits were residual feed intake, feed conversion ratio, ADG, and DMI. Methods of analysis were traditional BLUP, single-step genomic BLUP (ssGBLUP), genomic BLUP (GBLUP), and a Bayesian regression method (BayesCπ). Direct genomic values (DGV) from the last 2 methods were compared directly or in an index that combines DGV with parent average. Three cross-validation approaches were used to validate the models: 1) YOUNG, in which the partition into training and testing sets was based on year of birth and testing animals were born after 2010; 2) UNREL, in which the data set was split into 3 less related subsets and the validation was done in each subset a time; and 3) RANDOM, in which the data set was randomly divided into 4 subsets (considering the contemporary groups) and the validation was done in each subset at a time. On average, the RANDOM design provided the most accurate predictions. Average accuracies ranged from 0.10 to 0.58 using BLUP, from 0.09 to 0.48 using GBLUP, from 0.06 to 0.49 using BayesCπ, and from 0.22 to 0.49 using ssGBLUP. The most accurate and consistent predictions were obtained using ssGBLUP for all analyzed traits. The ssGBLUP seems to be more suitable to obtain genomic predictions for feed efficiency traits on an experimental population of genotyped animals.

Relação entre circunferência escrotal de touros Nelore e taxa de prenhez de vacas em monta natural / Relationship between scrotal circumference of nellore bulls and cow pregnancy rate in natural mating

A taxa de prenhez é de extrema importância para a eficiência produtiva de bovinos de corte, e a fertilidade dos touros pode influenciar na taxa de prenhez das matrizes em monta natural. O objetivo deste trabalho foi avaliar a relação entre as características do exame andrológico, especialmente a circunferência escrotal (CE), de touros da raça Nelore (Bos indicus), selecionados para reprodução com base em maior ganho de peso pós-desmama, com a taxa de prenhez de vacas Nelore em estação de monta natural de 90 dias de duração. Foram avaliados registros de 7171 entradas na monta de 1923 vacas submetidas a 216 touros em 368 lotes de estação de monta a campo (touro único), durante os anos de 1995 a 2014, e a CE dos touros. A CE foi pré-ajustada para idade do touro (2,67±0,62 anos) na entrada da estação de monta, em modelo de regressão linear, sendo posteriormente expressa em 3 classes: classe 1: CE < 35 cm; classe 2: 35 ≥ CE < 37 cm; classe 3: CE ≥ 37. Mais da metade das observações de CE ficaram na classe 2, e 74,52% das exposições aos touros resultaram em prenhez, com mínimo de 0% e máximo de 100% de sucesso de prenhez nos 368 lotes de monta. Parte (n=33) dos 216 touros tinham registros de características obtidas em exame andrológico parcial [CE, volume, motilidade, vigor, concentração e morfologia espermática (defeitos maiores e menores)]. Verificou-se que a classe de valores intermediários de CE (classe 2: 35 ≥ CE < 37 cm) foi a de maior risco relativo para sucesso na prenhez. Na amostra dos 33 touros, a taxa de prenhez apresentou correlação moderada e positiva (r = 0,66) somente com a concentração espermática. A fraca relação entre as características do andrológico os touros e taxa de prenhez das vacas pode ser devido ao fato da amostra dos touros avaliados neste tipo de trabalho ser sempre viesada. Ou seja, só são considerados os touros que entraram em monta...(AU)

The pregnancy rate is extremely important for the productive efficiency of beef cattle and bull fertility can influence the pregnancy rate of cows in natural mating. The objective of this study was to evaluate the relationship of andrological traits, especially scrotal circumference (SC), of Nellore bulls (Bos indicus) selected for breeding based on greater postweaning weight gain with the pregnancy rate of naturally mated Nellore cows in a 90-day breeding season. Records of 7,171 entries into the breeding season of 1,923 cows exposed to 216 bulls in 368 mating batches in the field (single bull) from 1995 to 2014, as well as records of SC, were evaluated. Scrotal circumference was pre-adjusted for bull age (2.67±0.62 years) at the start of the breeding season using a linear regression model and was divided into three classes: class 1: SC < 35 cm; class 2: 35 ≥ SC < 37 cm; class 3: SC ≥ 37. More than half the observations of SC were in class 2 and 74.52% of the exposures to bulls resulted in pregnancy, with a minimum rate of successful pregnancy of 0% and a maximum rate of 100% in the 368 mating batches. Thirty-three of the 216 bulls had records of andrological traits [SC, sperm volume, motility, vigor, concentration and morphology (major and minor defects)]. The class of intermediate SC values (class 2: 35 ≥ SC < 37 cm) was associated with a higher relative risk of pregnancy success. In the sample of the 33 bulls, pregnancy rate showed a moderate positive correlation (r = 0.66) only with sperm concentration. The weak association between andrological traits of bulls and pregnancy rate might be due to the fact that the sample of bulls evaluated in this type of study will be always biased. In other words, only bulls that enter the breeding season are considered, which correspond to a small percentage of all males born. Consequently, the variation observed in these bulls does...(AU)

Relação entre circunferência escrotal de touros Nelore e taxa de prenhez de vacas em monta natural / Relationship between scrotal circumference of nellore bulls and cow pregnancy rate in natural mating

A taxa de prenhez é de extrema importância para a eficiência produtiva de bovinos de corte, e a fertilidade dos touros pode influenciar na taxa de prenhez das matrizes em monta natural. O objetivo deste trabalho foi avaliar a relação entre as características do exame andrológico, especialmente a circunferência escrotal (CE), de touros da raça Nelore (Bos indicus), selecionados para reprodução com base em maior ganho de peso pós-desmama, com a taxa de prenhez de vacas Nelore em estação de monta natural de 90 dias de duração. Foram avaliados registros de 7171 entradas na monta de 1923 vacas submetidas a 216 touros em 368 lotes de estação de monta a campo (touro único), durante os anos de 1995 a 2014, e a CE dos touros. A CE foi pré-ajustada para idade do touro (2,67±0,62 anos) na entrada da estação de monta, em modelo de regressão linear, sendo posteriormente expressa em 3 classes: classe 1: CE < 35 cm; classe 2: 35 ≥ CE < 37 cm; classe 3: CE ≥ 37. Mais da metade das observações de CE ficaram na classe 2, e 74,52% das exposições aos touros resultaram em prenhez, com mínimo de 0% e máximo de 100% de sucesso de prenhez nos 368 lotes de monta. Parte (n=33) dos 216 touros tinham registros de características obtidas em exame andrológico parcial [CE, volume, motilidade, vigor, concentração e morfologia espermática (defeitos maiores e menores)]. Verificou-se que a classe de valores intermediários de CE (classe 2: 35 ≥ CE < 37 cm) foi a de maior risco relativo para sucesso na prenhez. Na amostra dos 33 touros, a taxa de prenhez apresentou correlação moderada e positiva (r = 0,66) somente com a concentração espermática. A fraca relação entre as características do andrológico os touros e taxa de prenhez das vacas pode ser devido ao fato da amostra dos touros avaliados neste tipo de trabalho ser sempre viesada. Ou seja, só são considerados os touros que entraram em monta...

The pregnancy rate is extremely important for the productive efficiency of beef cattle and bull fertility can influence the pregnancy rate of cows in natural mating. The objective of this study was to evaluate the relationship of andrological traits, especially scrotal circumference (SC), of Nellore bulls (Bos indicus) selected for breeding based on greater postweaning weight gain with the pregnancy rate of naturally mated Nellore cows in a 90-day breeding season. Records of 7,171 entries into the breeding season of 1,923 cows exposed to 216 bulls in 368 mating batches in the field (single bull) from 1995 to 2014, as well as records of SC, were evaluated. Scrotal circumference was pre-adjusted for bull age (2.67±0.62 years) at the start of the breeding season using a linear regression model and was divided into three classes: class 1: SC < 35 cm; class 2: 35 ≥ SC < 37 cm; class 3: SC ≥ 37. More than half the observations of SC were in class 2 and 74.52% of the exposures to bulls resulted in pregnancy, with a minimum rate of successful pregnancy of 0% and a maximum rate of 100% in the 368 mating batches. Thirty-three of the 216 bulls had records of andrological traits [SC, sperm volume, motility, vigor, concentration and morphology (major and minor defects)]. The class of intermediate SC values (class 2: 35 ≥ SC < 37 cm) was associated with a higher relative risk of pregnancy success. In the sample of the 33 bulls, pregnancy rate showed a moderate positive correlation (r = 0.66) only with sperm concentration. The weak association between andrological traits of bulls and pregnancy rate might be due to the fact that the sample of bulls evaluated in this type of study will be always biased. In other words, only bulls that enter the breeding season are considered, which correspond to a small percentage of all males born. Consequently, the variation observed in these bulls does...

Growth curves of carcass traits obtained by ultrasonography in three lines of Nellore cattle selected for body weight.

The effect of selection for postweaning weight was evaluated within the growth curve parameters for both growth and carcass traits. Records of 2404 Nellore animals from three selection lines were analyzed: two selection lines for high postweaning weight, selection (NeS) and traditional (NeT); and a control line (NeC) in which animals were selected for postweaning weight close to the average. Body weight (BW), hip height (HH), rib eye area (REA), back fat thickness (BFT), and rump fat thickness (RFT) were measured and records collected from animals 8 to 20 (males) and 11 to 26 (females) months of age. The parameters A (asymptotic value) and k (growth rate) were estimated using the nonlinear model procedure of the Statistical Analysis System program, which included fixed effect of line (NeS, NeT, and NeC) in the model, with the objective to evaluate differences in the estimated parameters between lines. Selected animals (NeS and NeT) showed higher growth rates than control line animals (NeC) for all traits. Line effect on curves parameters was significant (P < 0.001) for BW, HH, and REA in males, and for BFT and RFT in females. Selection for postweaning weight was effective in altering growth curves, resulting in animals with higher growth potential.

Heritability of predicted daily enteric methane emissions from growing Nellore cattle.

The objective of this study was to estimate the heritability of predicted daily enteric methane emissions (PME) from growing Nellore cattle. Dry matter intake (DMI) records of 955 Nellore animals that were born between 2004 and 2013, which were obtained in a postweaning performance test lasting 83 ± 15 days, were used. The PME of each animal, obtained as MJ/day and converted to g/day, was estimated using three equations: PME1 (MJ/day) = 2.29 + 0.647 x DMI (kg/day), PME2 (MJ/day) = 3.96 + 0.561 x DMI (kg/day), and PME3 (MJ/day) = 4.41 + 0.50 x DMI (kg/day). The heritability (h2) of PME obtained using the three equations was identical to the h2 of DMI, regardless of whether the model included the effect of mid-test weight (h2 = 0.32 ± 0.069) or not (h2 = 0.48 ± 0.069). The equations were based exclusively on variations in DMI, and detected variations in this trait without taking into consideration individual differences in enteric methane emission caused by differences in fermentation and digestion capacity. Therefore, prediction equations of enteric methane emission from DMI are not adequate to estimate differences between animals.

Polymorphisms in the GHRL gene and their associations with traits of economic interest in beef cattle.

The hormone ghrelin is produced in the stomach wall, has an orexigenic function, stimulates growth hormone secretion, and affects the energy balance of the animal. Therefore, the ghrelin gene (GHRL) is considered to be a good candidate marker for the identification of traits of great economic importance in cattle, such as those associated with feed intake, growth, and carcass quality. The use of molecular genetic markers associated with such traits permits the earlier and more accurate identification of superior animals, thus reducing the interval between generations, and increasing the genetic gain. Six SNPs were found in the GHRL gene, located in intron 3, intron 4, and exon 5. The positions of the SNPs on the gene and the substitutions were: g.2184A>G, g.2347T>C, g.4469T>C, g.4548A>G, g.4663T>C, and g.4729T>C (GenBank accession No. JX565585). After analysis of linkage disequilibrium, association tests were performed between four SNPs with the traits year weight for males, yearling weight for females, dry matter intake, loin eye area, and rump fat thickness (P ≤ 0.05). Therefore, GHRL is an important candidate gene that may be used to identify genetic variations that influence traits of economic importance in beef cattle.

Efeito da suplementação com fontes de ácido graxos poliinsaturados em doadoras primíparas da raça Nelore durante o pré e pós-parto sobre número de oócitos obtidos in vivo e produção in vitro de embriões / Effect of pre- anc postcalving supplementation of primiparous Nellore donor cows with sources of polyunsarurated fatty acids on the number of oocytes obtained in vivo and in vitro embryo production

O objetivo do trabalho foi avaliar os efeitos das suplementações ricas em ácidos linoleico (n-6) e linolênico (n-3) protegidos ou não, no pré e pós-parto de fêmeas primíparas da raça Nelore sobre número de folículos, número total de complexos cúmulos oócitos (CCO) aspirados (OPU) e de oócitos susceptíveis ao cultivo (graus I, II e III) e a produção in vitro de embriões (PIVE). As doadoras foram divididas aleatoriamente em 3 grupos: Controle (n=7), Megalac-E® (n=8; 100 g/doadora/dia) e Linhaça (n=7; 1,0 kg/doadora/dia). As dietas foram fornecidas pelo menos 30 dias pré-parto e 75 dias pós-parto. Estes animais foram submetidos à OPU nos dias 30, 45, 60 e 75 do pós-parto. Os oócitos recuperados foram selecionados e os considerados susceptíveis ao cultivo submetidos aos procedimentos da PIVE. Os dados foram analisados em um delineamento inteiramente casualisado com medidas repetidas no tempo. Não foi detectado efeito das suplementações no número de folículos, no número de CCO recuperados e susceptíveis ao cultivo e na PIVE. No entanto, houve um aumento na taxa de oócitos susceptíveis ao cultivo recuperado pela OPU nos dias 60 e 75 do pós-parto em comparação aos dias 30 e 45. Além disso, a taxa de embriões produzidos in vitro foi maior a partir do dia 45 pós-parto. Em conclusão, a suplementação pré e pós-parto com 100 g/dia de Megalac-E® ou 1,0 kg/dia de torta de linhaça não altera o número de oócitos obtidos in vivo e a PIVE de doadoras primíparas da raça Nelore lactantes. No entanto, a PIVE melhora quando os programas são realizados após 45 dias pós-parto...(AU)

The objective of this study was to evaluate the effects of feeding primiparous Nellore cows supplements rich in protected or unprotected linoleic (n-6) and linolenic (n-3) acids before and after calving on the number of follicles, total number of cumulus-oocyte complexes (COCs) collected by aspiration (OPU) and oocytes suitable for culture (grades I, II and III), and in vitro embryo production (IVEP). The donor cows were randomly divided into three groups: control (n=7), Megalac-E® (n=8; 100 g/donor/day), and flaxseed (n=7; 1.0 kg/donor/day). The diets were offered at least 30 days precalving and 75 days postcalving. The animals were submitted to OPU on postcalving days 30, 45, 60 and 75. Recovered oocytes were selected and those considered suitable for culture were submitted to the IVEP procedures. The data were analyzed using a completely randomized design with repeated measures over time. There was no effect of supplementation on the number of follicles, the number of recovered COCs and those suitable for culture, or IVEP. However, the rate of oocytes suitable for culture recovered by OPU was higher on postcalving days 60 and 75 when compared to days 30 and 45. Additionally, the rate of embryos produced in vitro increased after postcalving day 45. In conclusion, pre- and postcalving supplementation with 100 g/day Megalac-E® or 1.0 kg/day flaxseed does not alter the number of oocytes obtained in vivo or IVEP rates of lactating primiparous Nellore donor cows. However, IVEP improves when the programs are carried out 45 days after calving...(AU)

Efeito da suplementação com fontes de ácido graxos poliinsaturados em doadoras primíparas da raça Nelore durante o pré e pós-parto sobre número de oócitos obtidos in vivo e produção in vitro de embriões / Effect of pre- anc postcalving supplementation of primiparous Nellore donor cows with sources of polyunsarurated fatty acids on the number of oocytes obtained in vivo and in vitro embryo production

O objetivo do trabalho foi avaliar os efeitos das suplementações ricas em ácidos linoleico (n-6) e linolênico (n-3) protegidos ou não, no pré e pós-parto de fêmeas primíparas da raça Nelore sobre número de folículos, número total de complexos cúmulos oócitos (CCO) aspirados (OPU) e de oócitos susceptíveis ao cultivo (graus I, II e III) e a produção in vitro de embriões (PIVE). As doadoras foram divididas aleatoriamente em 3 grupos: Controle (n=7), Megalac-E® (n=8; 100 g/doadora/dia) e Linhaça (n=7; 1,0 kg/doadora/dia). As dietas foram fornecidas pelo menos 30 dias pré-parto e 75 dias pós-parto. Estes animais foram submetidos à OPU nos dias 30, 45, 60 e 75 do pós-parto. Os oócitos recuperados foram selecionados e os considerados susceptíveis ao cultivo submetidos aos procedimentos da PIVE. Os dados foram analisados em um delineamento inteiramente casualisado com medidas repetidas no tempo. Não foi detectado efeito das suplementações no número de folículos, no número de CCO recuperados e susceptíveis ao cultivo e na PIVE. No entanto, houve um aumento na taxa de oócitos susceptíveis ao cultivo recuperado pela OPU nos dias 60 e 75 do pós-parto em comparação aos dias 30 e 45. Além disso, a taxa de embriões produzidos in vitro foi maior a partir do dia 45 pós-parto. Em conclusão, a suplementação pré e pós-parto com 100 g/dia de Megalac-E® ou 1,0 kg/dia de torta de linhaça não altera o número de oócitos obtidos in vivo e a PIVE de doadoras primíparas da raça Nelore lactantes. No entanto, a PIVE melhora quando os programas são realizados após 45 dias pós-parto...

The objective of this study was to evaluate the effects of feeding primiparous Nellore cows supplements rich in protected or unprotected linoleic (n-6) and linolenic (n-3) acids before and after calving on the number of follicles, total number of cumulus-oocyte complexes (COCs) collected by aspiration (OPU) and oocytes suitable for culture (grades I, II and III), and in vitro embryo production (IVEP). The donor cows were randomly divided into three groups: control (n=7), Megalac-E® (n=8; 100 g/donor/day), and flaxseed (n=7; 1.0 kg/donor/day). The diets were offered at least 30 days precalving and 75 days postcalving. The animals were submitted to OPU on postcalving days 30, 45, 60 and 75. Recovered oocytes were selected and those considered suitable for culture were submitted to the IVEP procedures. The data were analyzed using a completely randomized design with repeated measures over time. There was no effect of supplementation on the number of follicles, the number of recovered COCs and those suitable for culture, or IVEP. However, the rate of oocytes suitable for culture recovered by OPU was higher on postcalving days 60 and 75 when compared to days 30 and 45. Additionally, the rate of embryos produced in vitro increased after postcalving day 45. In conclusion, pre- and postcalving supplementation with 100 g/day Megalac-E® or 1.0 kg/day flaxseed does not alter the number of oocytes obtained in vivo or IVEP rates of lactating primiparous Nellore donor cows. However, IVEP improves when the programs are carried out 45 days after calving...

Prediction of retail beef yield and fat content from live animal and carcass measurements in Nellore cattle.

Data from 156 Nellore males were used to develop equations for the prediction of retail beef yield and carcass fat content, expressed as kilograms and as a percentage, from live animal and carcass measurements. Longissimus muscle area and backfat and rump fat thickness were measured by ultrasound up to 5 d before slaughter and fasted live weight was determined 1 d before slaughter. The same traits were obtained after slaughter. The carcass edible portion (CEP in kg and CEP% in percentage; n = 116) was calculated by the sum of the edible portions of primal cuts: hindquarter, forequarter, and spare ribs. Trimmable fat from the carcass boning process, with the standardization of about 3 mm of fat on retail beef, was considered to be representative of carcass fat content. Most of the variation in CEP was explained by fasted live weight or carcass weight (R(2) of 0.92 and 0.96); the same occurred for CEP% (R(2) of 0.15 and 0.13), and for CEP, the inclusion of LM area and fat thickness reduced the equation bias (lower value of Mallow's Cp statistics). For trimmable fat, most variation could be explained by weight or rump fat thickness. In general, the equations developed from live animal measurements showed a predictive power similar to the equations using carcass measurements. In all cases, the traits expressed as kilograms were better predicted (R(2) of 0.39 to 0.96) than traits expressed as a percentage (R(2) of 0.08 to 0.42).

Ranking of Nellore animals in cattle championships: genetic parameters and correlations with production traits.

Records of 17,141 Nellore cattle participating in cattle championships, born from 1994-2009, were used to estimate genetic parameters between animal rank in cattle championships, evaluated from weaning to 36 months of age as repeated traits, and growth, fertility, and carcass traits, evaluated at 365 days of age as single traits. Two traits were defined for animal rank in cattle championships: value 1 was attributed to animals ranked from 1st to 3rd place within the age category, and value 0 was assigned to the remaining animals (TOP3). Value 1 was attributed to animals ranked from 1st to 5th place within the age category and value 0 was assigned to the remaining animals (TOP5). The (co)variance components were estimated based on Bayesian inference under a 2-trait threshold-linear animal model. The posterior means of heritability estimated for TOP3 and TOP5 were 0.182 ± 0.010 and 0.260 ± 0.012, respectively, and their repeatabilities were 0.341 ± 0.007 and 0.400 ± 0.007, respectively. High-ranking animals generally presented higher breeding values for body weight, height, body length, and heart girth. The phenotypic correlations indicate that judges of cattle championships primarily rank animals based on weight and heart girth.

Precocidade sexual em bovinos Nelore avaliada por ultrassonografia testicular / Sexual precocity in Nelore bovines evaluated by testicular ultrasonography

The present study aimed to evaluate if there are differences in testicular parenchyma echogenicity between pre-pubescent and pubescent animals at the same age. Ultrasound examinations were performed in longitudinal and transversal planes of the testicles of 111 healthy Nelore bovines, at the ages of nine, 13 and 15 months. The EIV software calculated the echogenicity of the testicular parenchyma, which ranged from 0 (anechoic) to 100% (hyperechoic). Animals that had reached puberty at 15 months of age presented higher testicular echogenicity than the animals that had not reached puberty at the same age. These results suggest that testicular ultrasonography can be used as a predictor of sexual precocity.(AU)

Precocidade sexual em bovinos Nelore avaliada por ultrassonografia testicular / Sexual precocity in Nelore bovines evaluated by testicular ultrasonography

The present study aimed to evaluate if there are differences in testicular parenchyma echogenicity between pre-pubescent and pubescent animals at the same age. Ultrasound examinations were performed in longitudinal and transversal planes of the testicles of 111 healthy Nelore bovines, at the ages of nine, 13 and 15 months. The EIV software calculated the echogenicity of the testicular parenchyma, which ranged from 0 (anechoic) to 100% (hyperechoic). Animals that had reached puberty at 15 months of age presented higher testicular echogenicity than the animals that had not reached puberty at the same age. These results suggest that testicular ultrasonography can be used as a predictor of sexual precocity.(AU)

Body chemical composition of Nellore bulls with different residual feed intakes.

Empty body and carcass chemical compositions, expressed as content of water, ether extract, protein, minerals, and energy, were evaluated in Nellore bulls with different residual feed intakes (RFI). Forty-nine not castrated males, with 343 kg of average initial BW and 398 kg of average slaughter BW, were studied. Animals were divided in two subgroups: reference group (RG) and ad libitum feeding group. At the end of the adaptation period, animals of subgroup RG were slaughtered and the other animals were finished in individual pens for approximately 100 d, until they reached a subcutaneous fat thickness over the LM of 4 mm, and were slaughtered at an average age of 540 d. Body composition was obtained after grinding, homogenizing, sampling, analyzing, and combining blood, hide, head + feet, viscera, and carcass. Tissue deposition rates and chemical composition of gain were also measured based on gains estimated by comparative slaughter technique. No significant differences in slaughter BW (P = 0.8639), empty BW (P = 0.7288), HCW (P = 0.6563), or empty body and carcass rates of gain were observed between RFI groups, demonstrating that the low (-0.331 kg DM/d) and high (+0.325 kg DM/d) RFI animals presented similar body sizes and growth rates. No significant differences in empty body or carcass content of water, ether extract, protein, minerals, and energy were observed between the low and high RFI animals. And also there were no significant differences in empty BW or carcass gain, demonstrating that low and high RFI animals had a similar growth potential. More efficient animals (low RFI) consumed less feed than less efficient animals (high RFI) but presented similar body sizes, growth rates, and empty body and carcass chemical composition.

Reproductive tract development and puberty in two lines of Nellore heifers selected for postweaning weight.

The objective was to evaluate reproductive tract development (ovary and uterus) and onset of puberty in two lines of Nellore heifers (Bos indicus) selected for postweaning weight. A total of 123 heifers, including 46 from the control Nellore line (NeC) and 77 from the selection Nellore line (NeS) were used. Every 18 to 21 days from 12 to 24 months of age, average ovarian area (OVA), endometrial thickness (ETh), and diameter of the largest follicle in each ovary were evaluated (using transrectal ultrasonography), and body weight, hip height, and body condition score were measured. There were no differences between NeS and NeC heifers for ETh or OVA (P < 0.05). Genetic selection for higher postweaning weight had no negative influence on the onset of puberty, with 52% and 48% of NeC and NeS heifers, respectively, pubertal at 24 months of age (P = 0.49). Heifers that reached puberty at the end of the study were heavier (NeC, 296.9 vs. 276.7 kg; NeS, 343.5 vs. 327.9 kg; P < 0.01) and younger (NeC, 23.4 vs. 24.2 mo; NeS, 22.7 vs. 24.0 months; P < 0.01) than those that did not. Furthermore, heifers that were heavier at weaning reached puberty earlier. Pubertal heifers had a greater OVA (4.15 vs. 3.14 cm(2); P < 0.01) and ETh (12.15 vs. 9.93 mm; P < 0.01) than nonpubertal heifers. Taken together, OVA and ETh had positive effects (P < 0.01) on the onset of puberty and were suitable indicator traits of heifer sexual precocity in pasture management systems. However, selection for weight did not alter ovarian or endometrial development, or manifestation of puberty at 24 months of age. Among the growth traits studied, weaning weight and weight at puberty had significant positive effects on manifestation of first estrus.