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1.
Transl Oncol ; 20: 101412, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35395603

RESUMO

PURPOSE: To compare the consistency of one-dimensional Response Evaluation Criteria in Solid Tumors (1D-RECIST), two-dimensional WHO criteria (2D-WHO), and three-dimensional (3D) measurement for therapeutic response assessment of nasopharyngeal carcinoma (NPC). MATERIALS AND METHODS: Retrospective data of 288 newly diagnosed NPC patients were reviewed. Tumor size was assessed on magnetic resonance imaging (MRI) according to the 1D-RECIST, 2D-WHO, and 3D measurement criteria. Agreement between tumor responses was assessed using unweighted k statistics. The receiver operating characteristic (ROC) curve was used to determine the optimal cut-off point of the PTV. The Kaplan-Meier method and Cox regression were used for the survival analysis. RESULTS: The optimal cut-off point of the PTV for progression-free survival (PFS) was 29.6%. Agreement with PTV measurement was better for 1D measurement than for 2D and 3D measurements (kappa values of 0.646, 0.537, and 0.577 for 1D, 2D, and 3D measurements, respectively; P < 0.05). The area under the curve of the 1D measurement (AUC=0.596) was similar to that of the PTV measurement (AUC=0.621). Compared with 2D and 3D measurements, 1D measurement is superior for predicting prognosis in NPC (C-index of 0.672, 0.663, and 0.646 were for 1D, 2D, and 3D measurements, respectively; P < 0.005). Survival analysis showed that patients with non-responders had worse prognosis (P < 0.05). CONCLUSIONS: The 1D measurement more closely agreed with the PTV measurement than the 2D and 3D measurements for predicting therapeutic responses in NPC. Therefore, we recommend using the less time-consuming 1D-RECIST criteria in routine clinical practice.

2.
Fa Yi Xue Za Zhi ; 30(4): 250-2, 256, 2014 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-25434084

RESUMO

OBJECTIVE: To explore the effect of nitric oxide (NO) on the gene expression of hepatic TNF-α and IL-1ß by crush injury of rat's soft tissues. METHODS: Rats were randomly divided into sham group, crush group, crush+aminoguanidine (AG) group, and crush+L-arginine (L-Arg) group. Activities of ALT and AST as well as NO level in serum were measured. Gene expressions of TNF-α and IL-1ß were detected with RT-PCR. RESULTS: Obvious increase in TNF-α and IL-1ß mRNA expression was detected in the crush group compared with the sham group (P<0.05). After pretreated L-Arg, expressions of TNF-α and IL-1ß mRNA were markedly increased (P<0.05). After pretreated AG, those indices obviously decreased (P<0.05). Activities of ALT and AST enhanced and NO level increased in the crush group compared with the sham group (P<0.05). Pretreatment with L-Arg or AG led to substantial increased or reduced activities of ALT and AST as well as NO levels, respectively. CONCLUSION: Endogenous NO mediated TNF-α, IL-1ß mRNA up expression in liver induced by increased production of NO after crush injury of rat's soft tissues.


Assuntos
Interleucina-1beta/metabolismo , Óxido Nítrico/fisiologia , Fator de Necrose Tumoral alfa/metabolismo , Ferimentos e Lesões , Animais , Expressão Gênica , Fígado , RNA Mensageiro , Ratos
4.
Fa Yi Xue Za Zhi ; 29(2): 127-9, 2013 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-23930509

RESUMO

OBJECTIVE: To explore a new method in order to extract DNA from bones and teeth automatically. METHODS: Samples of 33 bones and 15 teeth were acquired by freeze-mill method and manual method, respectively. DNA materials were extracted and quantified from the triturated samples by AutoMate Express forensic DNA extraction system. RESULTS: DNA extraction from bones and teeth were completed in 3 hours using the AutoMate Express forensic DNA extraction system. There was no statistical difference between the two methods in the DNA concentration of bones. Both bones and teeth got the good STR typing by freeze-mill method, and the DNA concentration of teeth was higher than those by manual method. CONCLUSION: AutoMate Express forensic DNA extraction system is a new method to extract DNA from bones and teeth, which can be applied in forensic practice.


Assuntos
Osso e Ossos/química , Impressões Digitais de DNA/métodos , DNA/isolamento & purificação , Medicina Legal/métodos , Dente/química , Automação , Impressões Digitais de DNA/instrumentação , Humanos , Repetições de Microssatélites , Reação em Cadeia da Polimerase , Manejo de Espécimes/métodos
6.
Nan Fang Yi Ke Da Xue Xue Bao ; 31(7): 1273-6, 2011 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-21764713

RESUMO

OBJECTIVE: To identify the risk factors of contrast-induced nephropathy (CIN) after coronary interventional therapy (PCI) in patients with coronary heart disease and analyze the clinical outcomes and the preventive measures of CIN. METHODS: Ninety-one patients who developed CIN after PCI were retrospectively analyzed to identify the risk factors and explore the preventive measures. RESULTS: CIN was strongly associated with pre-procedural chronic renal failure, diabetes mellitus and administration of large-dose contrast. The incidence of cardiac mortality in hospital or in the follow-up at one year after PCI, and the incidence of myocardial infarction or major adverse cardiac events in the follow-up at one year were obviously higher in patients with CIN than those without CIN. CONCLUSION: Chronic renal failure, diabetes mellitus and large-dose contrast administration are 3 independent risk factors of CIN, which affects the prognosis of the patients. Reinforcement of a comprehensive perioperative management of PCI, especially a rigorous preoperative preparation, can be an important strategy for prevention of CIN.


Assuntos
Meios de Contraste/efeitos adversos , Doença das Coronárias/terapia , Nefropatias/induzido quimicamente , Nefropatias/prevenção & controle , Intervenção Coronária Percutânea/efeitos adversos , Idoso , China/epidemiologia , Feminino , Humanos , Nefropatias/epidemiologia , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Risco
8.
Pharmacogenet Genomics ; 19(3): 226-34, 2009 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19177029

RESUMO

OBJECTIVES: To develop a warfarin-dosing algorithm that could be combined with pharmacogenomic and demographic factors, and to evaluate its effectiveness in a randomized prospective controlled clinical trial. METHODS: A pharmacogenetics-based dosing model was derived using retrospective data from 266 Chinese patients and multiple linear regression analysis. To prospectively validate this model, 156 patients with an operation of heart valve replacement were enrolled and randomly assigned to the group of pharmacogenetics-guided or traditional dosing for warfarin therapy. All patients were followed up for 50 days after initiation of warfarin therapy. The log-rank test was compared with the time-to-event (Kaplan-Meier) curves. Cox proportional hazards-regression model was used to assess the hazard ratio of the time to reach stable dose. RESULTS: The linear regression model derived from the pharmacogenomic model correlated with 54.1% of warfarin dosing variance. The final multiple linear regression model included age, body surface area, VKORC1, and CYP2C9 genotype. The study showed that the hazard ratio for the time to reach stable dose was 1.932 for the traditional dosing group versus the model-based group and a close and highly significant relationship was observed to exist between the predicted and the actual warfarin dose (R=0.454). CONCLUSION: A pharmacogenetics-based dosing algorithm has been developed for improvement in the time to reach the stable dosing of warfarin. This model may be useful in helping the clinicians to prescribe warfarin with greater safety and efficiency.


Assuntos
Anticoagulantes/administração & dosagem , Hidrocarboneto de Aril Hidroxilases/genética , Povo Asiático/genética , Genótipo , Oxigenases de Função Mista/genética , Varfarina/administração & dosagem , Idoso , Algoritmos , Anticoagulantes/farmacologia , China , Citocromo P-450 CYP2C9 , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Análise de Regressão , Vitamina K Epóxido Redutases , Varfarina/farmacologia
9.
Nan Fang Yi Ke Da Xue Xue Bao ; 27(7): 1068-70, 2007 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-17666352

RESUMO

OBJECTIVE: To investigate the relation of the levels of C-reactive protein (CRP) and antibodies against oxidized low-density lipoprotein (anti-oxLDL) to with acute coronary syndrome (ACS). METHODS: The levels of CRP, anti-oxLDL and anti-LDL were measured and compared in 96 subjects including 26 with acute myocardial infarction (AMI), 29 with unstable angina pectoris (UAP), 20 with stable angina pectoris (SAP) and 21 control subjects to evaluate the relationship between CRP and anti-oxLDL. RESULTS: Both CRP and anti-oxLDL levels in patients with ACS, including AMI and UAP, were significantly higher than those in SAP patients and the control subjects (P<0.05), but the level of anti-LDL showed no significant difference between the groups (P>0.05). There was significant positive correlation between the levels of CRP and anti-oxLDL (P<0.001). CONCLUSION: The specific immune response to oxLDL may play an important role in the instability of plaque and the occurrence of ACS, and anti-oxLDL level may serve as an important specific marker for the instability of plaque.


Assuntos
Anticorpos/sangue , Anticorpos/imunologia , Proteína C-Reativa/metabolismo , Doença das Coronárias/sangue , Lipoproteínas LDL/imunologia , Idoso , Biomarcadores/sangue , Biomarcadores/metabolismo , Estudos de Casos e Controles , Doença das Coronárias/complicações , Doença das Coronárias/metabolismo , Doença das Coronárias/patologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
10.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue ; 18(3): 150-3, 2006 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-16524505

RESUMO

OBJECTIVE: To elucidate the receptor mechanisms underlying the modulation of lipopolysaccharide (LPS)-induced nuclear factor-kappaB (NF-kappaB) expression in human umbilical vein endothelial cell line ECV-304 cells by cholecystokinin octapeptide (CCK-8). METHODS: Human umbilical vein endothelial cell line ECV-304 cells were stimulated with vehicle, LPS, CCK-8 (10(-9)-10(-7) mol/L), CCK receptor non-specific antagonist proglumide, CCK-A receptor (CCK-AR) specific antagonist CR-1409 or CCK-B receptor (CCK-BR) specific antagonist CR-2945 singularly or in combination. The NF-kappaB p65 protein level was determined by Western blot and immunocytochemistry technique. RESULTS: LPS resulted in an increase in the up-regulatory expression and nuclear translocation of NF-kappaB p65 protein in ECV-304 compared with vehicle stimulation. CCK-8 obviously inhibited LPS-induced the changes in NF-kappaB p65 protein in a dose-dependent manner. The inhibitory effects of CCK-8 on NF-kappaB p65 protein expression were attenuated by proglumide>CR-2945>CR-1409. CONCLUSION: CCK-AR and CCK-BR are involved in the mediation of CCK-8 inhibitive regulation for LPS-induced NF-kappaB protein expression in ECV-04 cells, whereas the effect of CCK-BR are more than that of CCK-R.


Assuntos
Células Endoteliais/metabolismo , NF-kappa B/metabolismo , Receptor de Colecistocinina A/metabolismo , Receptor de Colecistocinina B/metabolismo , Sincalida/farmacologia , Benzodiazepinas/farmacologia , Células Cultivadas , Células Endoteliais/efeitos dos fármacos , Humanos , Lipopolissacarídeos/farmacologia , Proglumida/análogos & derivados , Proglumida/farmacologia , Receptor de Colecistocinina A/antagonistas & inibidores , Receptor de Colecistocinina B/antagonistas & inibidores , Veias Umbilicais/citologia
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