[Study of the methods of determining the isoflavones in herbs of Trifolium pratense extractive].

OBJECTIVE: To study the methods of determining the amount of Isoflavones in red clover extractive. METHOD: RP-HPLC is employed to determine the Isoflavones, with a C18 RP Column, a moble phase of MeOH-CH3CN-0.1%H3PO4 and a detection wavelength of 260 nm. RESULT: After being water-solved, the four groups of flavoes elements satisfactorily separated, the amount of feeding in a range of 0.024-0.336 microg which has a good linear relationship with the peaks, and the total isoflavones determining results repetition, RSD=10.1%. CONCLUSION: A simple, reliable and effective quality-control method is given.