RESUMO
OBJECTIVE: To elucidate the relationship between the malignant transformation of oral epithelial dysplasia and the expression of p53 and Ki-67 proteins. METHODS: The expression of p53 and Ki-67 proteins from 60 cases of oral epithelial dysplasia (28 cases of mild epithelial dysplasia, 32 cases of severe epithelial dysplasia) and 30 normal oral mucosa tissue was determined by immunihistochemistry, and a 5-year follow-up study was made. RESULTS: In normal epithelium, Ki-67 expression was only observed in a small number of cells, and no expression of p53 was detected. In the cases of mild epithelial dysplasia, low levels of Ki-67 and p53 expression were detected. In the cases of severe epithelial dysplasia, the expression of p53 and Ki-67 protein was significantly higher than that in the normal oral mucosa and in the mild epithelial cases (P < 0.05). There was a correlation between the malignant transformation and the mutation of p53, Ki-67 in the cases of oral epithelial dysplasia. CONCLUSION: Carcinogenesis of oral mucosa is a continuous and stepwise course; p53 and Ki-67 genes may play an important role in this course.
Assuntos
Antígeno Ki-67/biossíntese , Neoplasias Bucais/genética , Lesões Pré-Cancerosas/genética , Proteína Supressora de Tumor p53/biossíntese , Idoso , Transformação Celular Neoplásica/genética , Feminino , Seguimentos , Humanos , Antígeno Ki-67/genética , Masculino , Pessoa de Meia-Idade , Mucosa Bucal/metabolismo , Neoplasias Bucais/metabolismo , Mutação , Lesões Pré-Cancerosas/metabolismo , Proteína Supressora de Tumor p53/genéticaRESUMO
OBJECTIVE: To detect and type human papilloma virus (HPV) in the warts of oral mucosa from HIV-positive patients, and better understand the biological characters of these oral warts. METHODS: Polymerase chain reaction (PCR) was used to detect and type HPV infection by consensus HPV primers Gp5+/Gp6+ and specific HPV primers (HPV6/11, 16, 18, 31, 33) in 34 cases of oral mucosa warts from HIV-positive patients. RESULTS: The HPV infection rate was 88.2% by consensus HPV primers Gp5+/Gp6+; the HPV infection rate of HPV6/11, 16, 18, 31 was respectively 47.06%, 11.67%; 2.94%, and 5.88% by specific HPV primers. CONCLUSION: Most lesions of oral warts from HIV-positive patients are associated with the infection of HPV. The low risk HPV6/11 infection is more common than the high risk HPV16, 18, 31.
