RESUMO
OBJECTIVE: To study the effect and its possible mechanism of genistein on the cell cycle of human highly metastatic ovarian carcinoma HO-8910PM cells. METHODS: Trypan blue stain assay was used to examine the effect of genistein on proliferation of HO-8910PM cells after 24 hours treatment. The cell cycle was assessed by flowcytometry (FCM). The expression level of NF-kappaB (p65) and the level of VEGF were assessed by Western blot analysis. RESULTS: Genistein could inhibit the proliferation of HO-8910PM cell and block the cell cycle at G1 phase. The expession level of NF-kappaB (P65) protein decreased obviously in HO-8910PM cells treated with 25 approximately 100 micromol/L genistein for 24 hours, and the effect appeared in the experssion of VEGF. CONCLUSION: The effect on cell cycle of genistein is involved in the decreasing expression of NF kappaB (p65) and the level of VEGF.
