RESUMO
A two-site enzyme-linked immunosorbent assay (ELISA) based on the avidin-biotin system was developed to study circulating antigens in the sera of rats orally or intraperitoneally infected with L3 larvae of Anisakis simplex s.l. Somatic and excretory-secretory antigens were detected from 24 h post-infection in all infected rats. A direct relationship between the number of larvae inoculated and the concentration of antigens in the sera was demonstrated; however, the amount of antigen decreased with the course of the infection. Comparative analysis revealed that intraperitoneal inoculation is most appropriate for detecting excretory-secretory antigens in serum. The minimum concentrations of A. simplex antigen detectable were approximately 2.5 microg/ml for somatic and excretory-secretory antigens. This antigen detection assay may have application in the diagnosis of anisakiasis.
Assuntos
Anisaquíase/parasitologia , Anisakis/imunologia , Antígenos de Helmintos/sangue , Ensaio de Imunoadsorção Enzimática/métodos , Animais , Anisaquíase/diagnóstico , Ensaio de Imunoadsorção Enzimática/estatística & dados numéricos , Larva/imunologia , Masculino , Ratos , Ratos Wistar , Sensibilidade e EspecificidadeRESUMO
Se está llevando a cabo un estudio sobre la helmintofauna parásita de aves pertenecientes al género Turdus "zorzales" ( T. philomelos, T. iliacus, T. viscivorus y T. merula).En este trabajo se denuncia, por primera vez en nuestro país, el trematodo Cyclocoelum mutabile en los sacos aéreos de Turdus philomelos (zorzal común) y se realiza una breve descripción de su morfología (AU)
Assuntos
Animais , Trematódeos/isolamento & purificação , Aves/parasitologia , Helmintos/isolamento & purificação , Trematódeos/anatomia & histologia , Trematódeos/classificação , Helmintos/anatomia & histologia , Estômago de Aves/parasitologiaRESUMO
An epidemiological study of animal giardiosis was carried out in the province of Granada. In this study, 912 samples from dogs, 592 from cows, 1165 from sheep, and 574 from goats were analyzed, obtaining prevalences of 12.09%, 0.16%, 6.26% and 4.00%, respectively. Prevalence was studied in relation to sex of host, area within the province and seasonal change; in addition, the age factor was taken into account in the case of dogs.
Assuntos
Doenças dos Bovinos/epidemiologia , Doenças do Cão/epidemiologia , Giardíase/veterinária , Doenças das Cabras/epidemiologia , Doenças dos Ovinos/epidemiologia , Fatores Etários , Animais , Bovinos , Cães , Feminino , Giardíase/epidemiologia , Cabras , Masculino , Prevalência , Estações do Ano , Fatores Sexuais , Ovinos , Espanha/epidemiologiaRESUMO
Monoclonal antibodies were produced and characterized to the metacyclic trypomastigotes stage of Trypanosoma cruzi. All the monoclonal antibodies (GR-12C/5; GR-P2B/5; GR-F2B/1; GR-J2C/6; GR-E6B/3; GR-A5B/5) also recognized antigens of epimastigotes of T. cruzi. These antigens are associated with the plasma membrane, flagellum, and an intracellular structure located by the nucleus. Immunoprecipitation using the monoclonal antibodies GR-J2C/6 and GR-E6B/3 followed by SDS-PAGE analysis has led to the following results: GR-J2C/6 precipitated two molecules with apparent molecular weights of 58 and 60 kD; the antigen recognized by GR-E6B/3 was a molecule with molecular weight of 68 kD. Only two of the monoclonal antibodies (GR-12C/5 and GR-P2B/5) recognized antigens of promastigotes of both Leishmania mexicana and L. infantum, and are associated with the plasma membrane.
