Greenhouse gas formation during the ensiling process of grass and lucerne silage.

Silage is an essential global feedstuff and an emitter of greenhouse gases. However, few studies have examined the formation of carbon dioxide (CO2), nitrous oxide (N2O) and methane (CH4) during the ensiling process. This study aimed to record the course of gas concentrations in forage during the ensiling process and determine the temporal variation in the (microbiological) formation processes. Grass and lucerne, each with two different dry matter (DM) concentrations (four variants, each n = 3), were ensiled in laboratory-scale barrels (120 L). Gas samples were taken from the headspace of the barrels and analysed using a gas chromatograph. The measurement period included the first 49 days of the ensiling process and the measurement interval was 0.5-48.0 h. For all variants, a rapid increase in CO2 concentration and a one-time N2O concentration peak was observed between ensiling hours 36 and 96. Lower DM concentration led to significantly faster CO2 production (p < 0.05). Lucerne forage and higher DM concentrations led to significantly increased N2O concentrations (p < 0.05). The extensive measurements demonstrated that butyric acid formation by clostridia contributes to CH4 formation; thus, lucerne silage had a significantly higher concentration from ensiling day 13 (p < 0.05). Therefore, malfermentation actively contributes to the formation of greenhouse gases. The method described here provides further insights into greenhouse gas formation during the ensiling process and can thus help to improve ensiling research and management.

A new experimental setup for measuring greenhouse gas and volatile organic compound emissions of silage during the aerobic storage period in a special silage respiration chamber.

The aim of this study was to develop a new experimental setup to determine parallel the emissions of greenhouse gases (GHG) and volatile organic compounds (VOCs) from silage during the opening as well as the subsequent aerobic storage phase of the complete bale without wrapping film. For this purpose, a special silage respiration chamber was used in which a silage bale could be examined. The gas analysis (CO2, methanol, ethanol, ethyl acetate) of inlet, ambient and outlet air of the silage respiration chamber was carried out by photoacoustic spectroscopy. The gas samples taken inside the bale were analysed by gas chromatography for CO2, O2, CH4, and N2O. Three silage bales (grass and lucerne) as the smallest silage unit commonly used in practice were examined. The emission behaviour of the bales was recorded during experimental periods up to 55 days. The results allow a differentiation of the outgassing processes. On the one hand, gases produced during the anaerobic ensiling process (CO2, CH4, N2O) are released once in a large amount during the first experimental hours after opening the silage. On the other hand, a continuous outgassing process takes place, which is particularly true for the VOCs ethanol, methanol, and ethyl acetate, whereby VOC emissions increase with rising ambient air temperatures. In this study, the emissions during the first 600 experimental hours from the grass silage bale and lucerne silage bale were 2313 g and 2612 g CO2, 17.6 g and 145.2 g methanol, 132.3 g and 675.9 g ethanol, 55.1 g and 66.2 g ethyl acetate, respectively. Nevertheless, the focus of this study was on the technical recording of gas concentrations inside the silage bale itself and the emissions in the ambient air of the bale. For a better interpretation of the data, additional factors should be considered in further investigations.