RESUMO
The percentage of non-diploid epidermal cells was determined by flow cytometry following application of leukotriene B4 (LTB4) to human skin. Doses in the range 35-500 ng were shown to cause a marked increase in proliferation, the non-diploid cells reaching a maximum between 72 and 96 h after LTB4 application. No difference was observed between the response of healthy controls and the uninvolved skin of psoriatic patients. We suggest, therefore, that the hyperproliferation is a consequence of the physical disruption of the stratum corneum accompanying the rupture of microabscesses.
Assuntos
Abscesso/patologia , Epiderme/patologia , Leucotrieno B4/farmacologia , Psoríase/patologia , Abscesso/induzido quimicamente , Divisão Celular/efeitos dos fármacos , Epiderme/efeitos dos fármacos , Humanos , Dermatopatias/induzido quimicamente , Dermatopatias/patologiaRESUMO
The penetration of polymorphonuclear leukocytes (PMNs) into the epidermis following topical application of leukotriene B4 was assessed in the clinically uninvolved skin of psoriatic patients treated with methotrexate and of psoriatic patients without treatment, and in normal controls. Inhibition of PMN infiltration was observed in those patients treated with methotrexate while there was no significant difference between untreated psoriatic patients and normal controls.
