RESUMO
A scale of relative affinities of a series of 2'-deoxycytidine and cytidine (CD) derivatives was established based on the data of cross-reactivities of these compounds as well as the displacements obtained from a competitive ELISA. No correlation could be established between the nucleosides modifying structures and the affinities. This can be explained by the possibilities of the modifying structures of intra- and intermolecular nonimmunospecific interactions owing to their degree of functionalization.
Assuntos
Anticorpos Monoclonais , Citidina/análogos & derivados , Citidina/imunologia , DNA/análise , Desoxicitidina/análogos & derivados , Desoxicitidina/imunologia , Afinidade de Anticorpos , Citidina/análise , Desoxicitidina/análise , Ensaio de Imunoadsorção Enzimática/métodos , Relação Estrutura-AtividadeRESUMO
Colorimetric immunoenzymatic procedures using monoclonal antibodies to detect 5-bromo-2-deoxy-uridine-DNA in hybridization experiments are not sensitive enough. We have developed a chemiluminescent immunodot assay to improve the sensitivity of the immunoenzymatic procedure, and attained a suitable detection level for single-stranded DNA. This new chemiluminescent immunodot assay has also been used to detect our recently synthesized cytidine analogue by means of its corresponding monoclonal antibody.
