Contribution of impactor misalignment to the neurofunctional variability in porcine spinal cord contusion models.

Traumatic spinal cord lesions studies are often carried out with animal models or numerical simulations. Unfortunately, animal models usually present a high variability in severity and type of neurofunctional impairments following impact surgery. We postulate that the variability of outcomes is strongly dependent on the positioning and alignment of the impactor during the contusion. A finite elements model of the spinal cord, predicting the action potential (AP) conduction alteration, was proposed and used to perform nine numerical simulations of a 50 g weight dropped from 200 mm on the exposed spinal cord in its spinal canal. Simulations followed a 32 factorial design with impactor eccentricity and spinal cord tilt angle as factors on two outcomes: injured spinal cord area (AP < 10 % of its baseline, 1h post-injury), and asymmetry of injury (ratio of right/left injured area of both half spinal cord). Eccentricity contributed highly and significantly on both outcomes, but not tilt angle. Damaged axons were found in conscious motor, sensory, and unconscious proprioception tracts. Variability in impactor alignment beyond ±6.2 % of the spinal canal width affects neurofunctional outcomes, and careful assessment of the impactor course is therefore key when producing spinal cord injury by contusion.Clinical Relevance- A precision value is proposed to mitigate the contribution of impactor misalignment to neurofunctional variability in animal models, allowing the reduction of animal used in research. The proposed method of action potential conduction assessment could easily be implanted in human numerical models for the cross-study of patient's cases.

Gait analysis of a post induced traumatic spinal cord injury porcine model.

Porcine model constitutes a potential translational model to study traumatic spinal cord injuries (TSCI) considering its recent use in numerous studies. Recovery of the animal is currently monitored through a qualitative evaluation of the gait. Adding a quantitative evaluation might help to better assess the functional recovery of the animal. In this study, a new controlled method involving the use of an electro-magnetic actuator was used on a pig to induce a TSCI. Chronic monitoring was done using a quantitative analysis of the gait. Results show both, the injury of the pig and its functional recovery. This large animal model will help to provide a better understanding of injury and recovery mechanisms and thus could constitute a strong preclinical model for future therapeutic studies.Clinical Relevance- Methodology and results from this study would provide a better insight on the functional recovery after traumatic spinal cord injuries.

A novel spinal cord surrogate for the study of compressive traumatic spinal cord injuries.

Although in vitro studies are frequent for the study of traumatic spine and spinal cord injuries, few include the spinal cord due to its prompt post-mortem decay. Several materials have been proposed to mimic the spinal cord behaviour, but none matched its mechanical properties under transverse compression, which is vital for the study of burst fractures and other injury mechanisms leading to spinal cord compression. In this study, a new material named Soma Foama 15 (Reynolds Advanced Material, USA) was used to manufacture three spinal cord surrogates at 3 mixing ratios of elastomer to catalyst (1:1, 2:1 and 3:1) and tested at three different strain rates (0.5, 5 and 50 .s-1). The mixing ratio 3:1 presents a mechanical behaviour comparable to that of the porcine spinal cord at each of these strain rates, making the surrogate a valid substitute up to 75 % of transverse compression.

Biomechanical evaluation of pedicle screw loosening mechanism using synthetic bone surrogate of various densities.

Pedicle screw fixation is a well-established procedure for various spinal disorders. However, pedicle screws failures are still reported. Therefore, there is a need for a greater understanding of the pedicle screw failure mechanism. This experimental study investigates the biomechanical stability of pedicle screws using a synthetic bone surrogate with a special focus on the screw loosening mechanism. Pedicle screws have been inserted in thirty six polyurethane foam blocks of three different densities. In half of the specimens from each density group, pedicle screws were submitted to cyclic bending (toggling) before pullout. The rest of specimens were solely loaded in axial pullout. The peak pullout force and stiffness were determined from load-displacement curve of each specimen. Statistical analyses were performed to investigate on the effect of toggling and bone surrogate density on the pedicle screw's pullout force. The results suggest that the pullout force and stiffness were significantly affected by toggling and density. Higher pullout forces resulted from higher grades of density. The proposed method allowed investigating the pedicle screw loosening mechanism. However, conducing further experimental tests on animal or cadaveric vertebrae are needed to confirm these findings.

Estimation of pedicle screw fixation strength from probe indentation force and screw insertion torque: a biomechanical study on bone surrogates of various densities.

Posterior pedicle screw fixation is commonly used for patients with spinal disorders. However, failure of fixation is reported in many cases and surgeons have only little information. The objective of this study was to assess the correlation between the probe indentation force, screw insertion torque and the pullout force using bone surrogates of different densities. The indentation force and insertion torque were measured using a custom made test bench during screw insertion into polyurethane foam blocks. The two variables were significantly correlated to pullout force and to density. A high correlation was also found between indentation force and the peak insertion torque. The proposed methods for measuring indentation force and screw insertion torque were reproducible. This study suggests that the peak screw insertion torque and the indentation force can predict the screw fixation strength in synthetic bone models. Additional tests should be performed on animal and human specimens to confirm and to translate these findings to clinical applications.

Biomechanical analysis of proximal junctional kyphosis: preliminary results.

Proximal junctional kyphosis (PJK) is a complication related to spinal instrumentation. Defined as a pathological kyphosis of levels above the upper instrumented vertebra, PJK is not yet fully understood although many studies have been conducted in this regard. The objective of this study is therefore to understand the influence of some biomechanical risk factors with respect to PJK. For this purpose, a biomechanical model has been developed and used to test three factors: the sagittal balance, the implant type used at the upper instrumented vertebra (UIV) and the proximal dissection (posterior ligaments disruption and joint capsule degeneration). The preliminary results showed that the PJK is correlated to both sagittal balance and the reduction of spinal stability due to posterior ligaments disruption and joint capsule degeneration.

Thoracic pedicle screw insertion using a transpedicular drill guide: a preliminary study.

Insertion of thoracic pedicle screws can lead to major complications. This study reports the use of a transpedicular drill guide (TDG) for safe pedicle screw insertion in the thoracic spine. The conventional anatomic technique and the TDG were both used to drill pilot holes into the pedicles of four anatomic models of the thoracic spine. Ninety-nine percent of the 96 pilot holes drilled with the TDG were within 2 mm from the pedicle wall compared with 79% for the anatomic technique (P < 0.001). The TDG reduced the proportion and the extent of medial perforations. The TDG was easy to use and was superior to the conventional anatomic technique. It could be combined with fluoroscopy and pedicle palpation in certain clinical applications, especially for training surgeons, but only after confirming its accuracy in a cadaveric study.

Intra-operative tracking of the trunk during surgical correction of scoliosis: a feasibility study.

OBJECTIVE: The purpose of this study was to evaluate the feasibility of a technique for intra-operative tracking of the trunk during scoliosis surgery. MATERIALS AND METHODS: Eleven magnetic sensors placed on specific anatomical landmarks are used to compute 11 geometric indices of the trunk. This technique was assessed on a cohort of 40 subjects (19 normal, 21 scoliotic) using an experimental set-up simulating the position of patients during scoliosis surgery. RESULTS: The indices varied less than 2 mm and 1 degrees when breathing (except for chest AP diameter), and less than 1 mm and 1 degrees after transient displacement from the initial positioning of the subjects. No significant changes were observed for most of the indices between two acquisition sessions. Comparison between normal and scoliotic subjects demonstrated that the trunk geometry is more influenced by the positioning of each subject on the operating table than by the magnitude of the spinal deformity. CONCLUSION: The proposed technique will allow intra-operative tracking of the trunk and enable the surgeon to optimize the correction of both spinal and trunk deformities. The technique can also be used to evaluate the adequacy of patient positioning on the operating table, and to obtain a complete follow-up of patients in pre-, intra-, and post-surgical conditions.

Registration and geometric modelling of the spine during scoliosis surgery: a comparison study of different pre-operative reconstruction techniques and intra-operative tracking systems.

During scoliosis instrumentation surgery, it is difficult for surgeons fully to track vertebral motion in 3D, because only the posterior elements of the spine are exposed. Different intra-operative modelling approaches are evaluated using a registration technique that matches intra-operative measurements with a 3D pre-operative model of the spine. Two tracking systems (magnetic digitiser and mechanical arm) and two pre-operative reconstruction techniques (multiplanar radiography and CT scan) are sequentially combined to build four intra-operative models. Their accuracy is assessed by comparison with the pre-operative geometry. The most minimally invasive approach (multiplanar radiographic reconstruction and magnetic digitiser) has an accuracy of 5.9 mm in translation, and errors on vertebral rotations are 4.4 degrees, 6.7 degrees and 5.0 degrees in the frontal, sagittal and transverse planes, respectively. With CT scan reconstruction, the accuracy is significantly increased by about 2 mm in translation and as much as 4.5 degrees for vertebral rotations in the sagittal plane. For the mechanical arm, the accuracy is increased by less than 1 mm in translation and 1 degree for vertebral rotations. CT scan is the most accurate reconstruction technique, but its use for long spinal segments is generally not allowed because of the high radiation exposure. Multiplanar radiographic reconstruction may be an alternative solution for long spinal segments when great accuracy is not necessary. Considering the small increase in accuracy and its awkwardness, the use of the mechanical arm may not be appropriate during surgical manoeuvres.