A misleading tail: A long-term study of reptile responses to multiple disturbances undermined by a change in surveying techniques.

Long-term ecological monitoring is crucial to understanding the complex dynamics of ecosystems, communities, and populations. Despite this, monitoring data are lacking or rare for the vast majority of biodiversity. Here we report the results of 19 years (2003-2022) of continuous annual monitoring of reptile species at Booderee National Park (BNP) on the east coast of south-eastern Australia. We tested the effects of time, habitat type, fire, and climate on detections of five reptile species. Our study revealed declines in detections of two skink species over time (Lampropholis delicata and Ctenotus taeniolatus), which we suspect was partly driven by weather conditions influencing activity of these species. We also identified broad vegetation type associations for two congeneric species with L. delicata being associated with forested sites, and Lampropholis guichenoti associated with more shrubby sites. Our results also demonstrated a clear association between Cryptophis nigrescens and L. delicata and fire, with the probabilities of detection of both species decreasing with time since fire in the short term. At about the midway point of our study (in 2011), we were forced to make a change in the way our data were collected. The change heavily influenced our findings, and so breached the integrity of the time series in our dataset. We acknowledge that a simple but crucial step to mitigate this breach would have been to conduct calibration that allowed subsequent analysis to control for a change in field survey methodology. Whilst improvements in the effectiveness of field survey methods might be possible through new technologies, it is crucial to maintain the integrity of long-term datasets as data collection continues.

Environmental variables influence patterns of mammal co-occurrence following introduced predator control.

Co-occurring species often overlap in their use of resources and can interact in complex ways. However, shifts in environmental conditions or resource availability can lead to changes in patterns of species co-occurrence, which may be exacerbated by global escalation of human disturbances to ecosystems, including conservation-directed interventions. We investigated the relative abundance and co-occurrence of two naturally sympatric mammal species following two forms of environmental disturbance: wildfire and introduced predator control. Using 14 years of abundance data from repeat surveys at long-term monitoring sites in south-eastern Australia, we examined the association between a marsupial, the common brushtail possum Trichosurus vulpecula, and a co-occurring native rodent, the bush rat Rattus fuscipes. We asked: In a fox-controlled environment, are the abundances of common brushtail possums and bush rats affected by environmental disturbance and each other's presence? Using Bayesian regression models, we tested hypotheses that the abundance of each species would vary with changes in environmental and disturbance variables, and that the negative association between bush rats and common brushtail possums was stronger than the association between bush rats and disturbance. Our analyses revealed that bush rat abundance varied greatly in relation to environmental and disturbance variables, whereas common brushtail possums showed relatively limited variation in response to the same variables. There was a negative association between common brushtail possums and bush rats, but this association was weaker than the initial decline and subsequent recovery of bush rats in response to wildfires. Using co-occurrence analysis, we can infer negative relationships in abundance between co-occurring species, but to understand the impacts of such associations, and plan appropriate conservation measures, we require more information on interactions between the species and environmental variables. Co-occurrence can be a powerful and novel method to diagnose threats to communities and understand changes in ecosystem dynamics.

Post-fire pickings: Large herbivores alter understory vegetation communities in a coastal eucalypt forest.

Fire and herbivores alter vegetation structure and function. Future fire activity is predicted to increase, and quantifying changes in vegetation communities arising from post-fire herbivory is needed to better manage natural environments. We investigated the effects of post-fire herbivory on understory plant communities in a coastal eucalypt forest in southeastern Australia. We quantified herbivore activity, understory plant diversity, and dominant plant morphology following a wildfire in 2017 using two sizes of exclosures. Statistical analysis incorporated the effect of exclusion treatments, time since fire, and the effect of a previous prescribed burn. Exclusion treatments altered herbivore activity, but time since fire did not. Herbivory reduced plant species richness, diversity, and evenness and promoted the dominance of the most abundant plants within the understory. Increasing time since fire reduced community diversity and evenness and influenced morphological changes to the dominant understory plant species, increasing size and dead material while decreasing abundance. We found the legacy effects of a previous prescribed burn had no effect on herbivores or vegetation within our study. Foraging by large herbivores resulted in a depauperate vegetation community. As post-fire herbivory can alter vegetation communities, we postulate that management burning practices may exacerbate herbivore impacts. Future fire management strategies to minimize herbivore-mediated alterations to understory vegetation could include aggregating management burns into larger fire sizes or linking fire management with herbivore management. Restricting herbivore access following fire (planned or otherwise) can encourage a more diverse and species-rich understory plant community. Future research should aim to determine how vegetation change from post-fire herbivory contributes to future fire risk.

The effects of 0.9% saline versus Plasma-Lyte 148 on renal function as assessed by creatinine concentration in patients undergoing major surgery: A single-centre double-blinded cluster crossover trial.

OBJECTIVES: Saline and Plasma-Lyte have different physiochemical contents; consequently, they may differently affect patients' renal function. We compared the effects of fluid therapy with 0.9% saline and with Plasma-Lyte 148 on renal function as assessed by creatinine concentration among patients undergoing major surgery. METHODS: We conducted a prospective, double-blinded cluster crossover trial comparing the effects of the two fluids on major surgery patients. The primary aim was to establish the pilot feasibility, safety and preliminary efficacy evidence base for a large interventional trial to establish whether saline or Plasma-Lyte is the preferred crystalloid fluid for managing major surgery patients. The primary efficacy outcome was the proportion of patients with changes in renal function as assessed by creatinine concentration during their index hospital admission. We used changes in creatinine to define acute kidney injury (AKI) according to the RIFLE criteria. RESULTS: The study was feasible with 100% patient and clinician acceptance. There were no deviations from the trial protocol. After screening, we allocated 602 patients to saline and 458 to Plasma-Lyte. The median (IQR) volume of intraoperative fluid received was 2000 mL (1000:2000) in both groups. Forty-nine saline patients (8.1%) and 49 Plasma-Lyte patients (10.7%) developed a postoperative AKI (adjusted incidence rate ratio [aIRR]: 1.34; 95% CI: 0.93-1.95; p = 0.120). No differences were observed in the development of postoperative complications (aIRR: 0.98; 95% CI: 0.89-1.08) or the severity of the worst complication (aIRR: 1.00; 95% CI: 0.78-1.30). The median (IQR) length of hospital stay was six days (3:11) for the saline group and five days (3:10) for the Plasma-Lyte group (aIRR: 0.85; 95% CI: 0.73-0.98). There were no serious adverse events relating to the trial fluids, nor were there fluid crossover or contamination events. CONCLUSIONS: The study design was feasible to support a future follow-up larger clinical trial. Patients treated with saline did not demonstrate an increased incidence of postoperative AKI (defined as changes in creatinine) compared to those treated with Plasma-Lyte. Our findings imply that clinicians can reasonably use either solution intraoperatively for adult patients undergoing major surgery. TRIAL REGISTRATION: Registry: Australian New Zealand Clinical Trials Registry; ACTRN12613001042730; URL: https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=364988.

BASELINE HEALTH PARAMETERS FOR A NEWLY ESTABLISHED POPULATION OF LONG-NOSED POTOROO (POTOROUS TRIDACTYLUS) AT BOODEREE NATIONAL PARK, AUSTRALIA.

Over two field seasons during 2014-15, 35 long-nosed potoroos (Potorous tridactylus) were captured in state forests in South Eastern New South Wales for translocation to Booderee National Park, Jervis Bay Territory, Australia. Animals were anesthetized for physical examination and collection of samples to assess general health and screen for select diseases identified during a disease risk assessment. Morphologic, hematologic, and biochemical parameters were determined, and parasites were identified where possible. Trypanosoma gilletti, Trypanosoma vegrandis, and novel genotypes most similar to a Trypanosoma wallaby-derived isolate (ABF) were identified from blood samples by PCR; the first time Trypanosoma has been described in this species. Also reported is the first confirmation of the Australian paralysis tick, Ixodes holocyclus, from the long-nosed potoroo. Surveillance showed that Cryptococcus sp. may form part of the normal nasal flora for long-nosed potoroo. Salmonella enterica serotype Dublin and Salmonella enterica subsp. enterica was identified from rectal swabs of otherwise healthy animals. The data provide baseline health and disease parameters for this newly established population and the source population and will inform future translocation and conservation management activities. These data expand current knowledge on aspects of the biology and microbiology of the long-nosed potoroo, both locally and nationally.

Can evolutionary theories of dispersal and senescence predict postrelease survival, dispersal, and body condition of a reintroduced threatened mammal?

Theories of dispersal and senescence (or aging) predict that dispersal, and ongoing survival and body condition, are influenced by evolutionary drivers, along with intrinsic and extrinsic factors. Such theories are relevant to translocations of animals where high mortality, loss of body condition, and dispersal beyond the area of release are commonly reported. However, these theories have rarely been tested using data from translocations.We explore whether theories of dispersal and senescence, together with biological knowledge and management interventions, can predict rates of postrelease dispersal, survival and change in body condition of a translocated endangered meso-predator, the eastern quoll Dasyurus viverrinus.Captive-bred quolls (n = 60) from three sanctuaries were translocated to an unfenced, predator-managed reserve (Booderee National Park) over 2 years (2018, 2019). Survival, dispersal and body mass were monitored via GPS/VHF tracking and targeted trapping for 45 days postrelease.We found support for the "social subordinate" hypothesis, with smaller quolls dispersing further. Consistent with theories of senescence and the biology of our species, survival was marginally greater for females, and females regained losses in body mass in both years following release. In contrast, males recovered body condition in the first but not the second release as this coincided with breeding. Quolls that originated from the mainland sanctuary were on average heavier at release and, after accounting for weight, dispersed further. Synthesis and applications. Using theory to test outcomes of wildlife translocations can provide insights into patterns across taxa and under different conditions, enabling useful improvements to future fauna translocations. This allows for better predictions to be made about the likelihood of success from proposed translocations, changes to planning to improve outcomes (e.g., modifying sex ratios, individual selection and release cohort), and improved animal welfare as fewer animals are subjected to trials.

Finding food in a novel environment: The diet of a reintroduced endangered meso-predator to mainland Australia, with notes on foraging behaviour.

Translocated captive-bred predators are less skilled at hunting than wild-born predators and more prone to starvation post-release. Foraging in an unfamiliar environment presents many further risks to translocated animals. Knowledge of the diet and foraging behaviour of translocated animals is therefore an important consideration of reintroductions. We investigated the diet of the endangered meso-predator, the eastern quoll Dasyurus viverrinus. We also opportunistically observed foraging behaviour, enabling us to examine risks associated with foraging. Sixty captive-bred eastern quolls were reintroduced to an unfenced reserve on mainland Australia (where introduced predators are managed) over a two year period (2018, 2019). Quolls were supplementary fed macropod meat but were also able to forage freely. Dietary analysis of scats (n = 56) revealed that quolls ate macropods, small mammals, birds, invertebrates, fish, reptiles and frogs, with some between-year differences in the frequency of different diet categories. We also observed quolls hunting live prey. Quolls utilised supplementary feeding stations, indicating that this may be an important strategy during the establishment phase. Our study demonstrated that, in a novel environment, captive-bred quolls were able to locate food and hunt live prey. However, foraging was not without risks; with the ingestion of toxic substances and foraging in dangerous environments found to be potentially harmful. Knowledge of the diet of reintroduced fauna in natural landscapes is important for understanding foraging behaviour and evaluating habitat suitability for future translocations and management.

Invasive shrub re-establishment following management has contrasting effects on biodiversity.

Effective control of an invasive species is frequently used to infer positive outcomes for the broader ecosystem. In many situations, whether the removal of an invasive plant is of net benefit to biodiversity is poorly assessed. We undertook a 10-year study on the effects of invasive shrub management (bitou bush, Chrysanthemoides monilifera ssp. rotundata) on native flora and fauna in a eucalypt forest in south-eastern Australia. Bitou bush eradication is a management priority, yet the optimal control regime (combination of herbicide spray and fire) is difficult to implement, meaning managed sites have complex management histories that vary in effectiveness of control. Here we test the long-term response of common biodiversity indicators (species richness, abundance and diversity of native plants, birds, herpetofauna and small mammals) to both the management, and the post-management status of bitou bush (% cover). While average bitou bush cover decreased with management, bitou bush consistently occurred at around half of our managed sites despite control efforts. The relationship between biodiversity and bitou bush cover following management differed from positive, neutral or negative among species groups and indicators. Native plant cover was lower under higher levels of bitou bush cover, but the abundance of birds and small mammals were positively related to bitou bush cover. Evidence suggests that the successful control of an invader may not necessarily result in beneficial outcomes for all components of biodiversity.

A novel approach to assessing the ecosystem-wide impacts of reintroductions.

Reintroducing a species to an ecosystem can have significant impacts on the recipient ecological community. Although reintroductions can have striking and positive outcomes, they also carry risks; many well-intentioned conservation actions have had surprising and unsatisfactory outcomes. A range of network-based mathematical methods has been developed to make quantitative predictions of how communities will respond to management interventions. These methods are based on the limited knowledge of which species interact with each other and in what way. However, expert knowledge isn't perfect and can only take models so far. Fortunately, other types of data, such as abundance time series, is often available, but, to date, no quantitative method exists to integrate these various data types into these models, allowing more precise ecosystem-wide predictions. In this paper, we develop mathematical methods that combine time-series data of multiple species with knowledge of species interactions and we apply it to proposed reintroductions at Booderee National Park in Australia. There have been large fluctuations in species abundances at Booderee National Park in recent history, following intense feral fox (Vulpes vulpes) control, including the local extinction of the greater glider (Petauroides volans). These fluctuations can provide information about the system isn't readily obtained from a stable system, and we use them to inform models that we then use to predict potential outcomes of eastern quoll (Dasyurus viverrinus) and long-nosed potoroo (Potorous tridactylus) reintroductions. One of the key species of conservation concern in the park is the Eastern Bristlebird (Dasyornis brachypterus), and we find that long-nosed potoroo introduction would have very little impact on the Eastern Bristlebird population, while the eastern quoll introduction increased the likelihood of Eastern Bristlebird decline, although that depends on the strength and form of any possible interaction.

Do temporal changes in vegetation structure additional to time since fire predict changes in bird occurrence?

Fire is a major ecological process in ecosystems globally. Its impacts on fauna can be both direct (e.g., mortality) and indirect (e.g., altered habitat), resulting in population recovery being driven by several possible mechanisms. Separating direct from indirect impacts of fire on faunal population recovery can be valuable in guiding management of biodiversity in fire-prone environments. However, resolving the influence of direct and indirect processes remains a key challenge because many processes affecting fauna can change concomitantly with time since fire. We explore the mechanisms influencing bird response to fire by posing the question, can temporal changes in vegetation structure predict changes in bird occurrence on sites, and can these be separated from other temporal changes using the surrogate of time since fire? We conducted a 12-yr study of bird and vegetation responses to fire at 124 sites across six vegetation classes in Booderee National Park, Australia. Approximately half of these sites, established in 2002, were burned by a large (>3000 ha) wildfire in 2003. To disentangle collinear effects of temporal changes in vegetation and direct demographic effects on population recovery that are subsumed by time since fire, we incorporated both longitudinal and cross-sectional vegetation effects in addition to time since fire within logistic structural equation models. We identified temporal changes in vegetation structure and richness of plant and bird species that characterized burned and unburned sites in all vegetation classes. For nine bird species, a significant component of the year trend was driven by temporal trends in one of three vegetation variables (number of understory or midstory plant species, or midstory cover). By contrast, we could not separate temporal effects between time since fire and vegetation attributes for bird species richness, reporting rate, and the occurrence of 11 other bird species. Our findings help identify species for which indirect effects of vegetation dominate recovery and thus may benefit from vegetation management where conservation actions are required and, conversely, those species for which direct effects of time since fire drive recovery, where simply leaving a system to recover following the last disturbance will be sufficient.

Temporal trends in mammal responses to fire reveals the complex effects of fire regime attributes.

Fire is a major ecological process in many ecosystems worldwide. We sought to identify which attributes of fire regimes affect temporal change in the presence and abundance of Australian native mammals. Our detailed study was underpinned by time series data on 11 mammal species at 97 long-term sites in southeastern Australia between 2003 and 2013. We explored how temporal aspects of fire regimes influenced the presence and conditional abundance of species. The key fire regime components examined were: (1) severity of a major fire in 2003, (2) interval between the last major fire (2003) and the fire prior to that, and (3) number of past fires. Our long-term data set enabled quantification of the interactions between survey year and each fire regime variable: an ecological relationship missing from temporally restricted studies. We found no evidence of any appreciable departures from the assumption of independence of the sites. Multiple aspects of fire regimes influenced temporal variation in the presence and abundance of mammals. The best models indicated that six of the 11 species responded to two or more fire regime variables, with two species influenced by all three fire regime attributes. Almost all species responded to time since fire, either as an interaction with survey year or as a main effect. Fire severity or its interaction with survey year was important for most terrestrial rodents. The number of fires at a site was significant for terrestrial rodents and several other species. Our findings contain evidence of the effects on native mammals of heterogeneity in fire regimes. Temporal response patterns of mammal species were influenced by multiple fire regime attributes, often in conjunction with survey year. This underscores the critical importance of long-term studies of biota that are coupled with data sets characterized by carefully documented fire history, severity, and frequency. Long-term studies are essential to predict animal responses to fires and guide management of when and where (prescribed) fire or, conversely, long-unburned vegetation is needed. The complexity of observed responses highlights the need for large reserves in which patterns of heterogeneity in fire regimes can be sustained in space and over time.

A long-term experimental case study of the ecological effectiveness and cost effectiveness of invasive plant management in achieving conservation goals: bitou bush control in booderee national park in eastern australia.

Invasive plant management is often justified in terms of conservation goals, yet progress is rarely assessed against these broader goals, instead focussing on short-term reductions of the invader as a measure of success. Key questions commonly remain unanswered including whether invader removal reverses invader impacts and whether management itself has negative ecosystem impacts. We addressed these knowledge gaps using a seven year experimental investigation of Bitou Bush, Chrysanthemoides monilifera subsp. rotundata. Our case study took advantage of the realities of applied management interventions for Bitou Bush to assess whether it is a driver or passenger of environmental change, and quantified conservation benefits relative to management costs of different treatment regimes. Among treatments examined, spraying with herbicide followed by burning and subsequent re-spraying (spray-fire-spray) proved the most effective for reducing the number of individuals and cover of Bitou Bush. Other treatment regimes (e.g. fire followed by spraying, or two fires in succession) were less effective or even exacerbated Bitou Bush invasion. The spray-fire-spray regime did not increase susceptibility of treated areas to re-invasion by Bitou Bush or other exotic species. This regime significantly reduced plant species richness and cover, but these effects were short-lived. The spray-fire-spray regime was the most cost-effective approach to controlling a highly invasive species and facilitating restoration of native plant species richness to levels characteristic of uninvaded sites. We provide a decision tree to guide management, where recommended actions depend on the outcome of post-treatment monitoring and performance against objectives. Critical to success is avoiding partial treatments and treatment sequences that may exacerbate invasive species impacts. We also show the value of taking advantage of unplanned events, such as wildfires, to achieve management objectives at reduced cost.

Geographical variation in body size and sexual size dimorphism in an Australian lizard, Boulenger's Skink (Morethia boulengeri).

Ecogeographical rules help explain spatial and temporal patterns in intraspecific body size. However, many of these rules, when applied to ectothermic organisms such as reptiles, are controversial and require further investigation. To explore factors that influence body size in reptiles, we performed a heuristic study to examine body size variation in an Australian lizard, Boulenger's Skink Morethia boulengeri from agricultural landscapes in southern New South Wales, south-eastern Australia. We collected tissue and morphological data on 337 adult lizards across a broad elevation and climate gradient. We used a model-selection procedure to determine if environmental or ecological variables best explained body size variation. We explored the relationship between morphology and phylogenetic structure before modeling candidate variables from four broad domains: (1) geography (latitude, longitude and elevation), (2) climate (temperature and rainfall), (3) habitat (vegetation type, number of logs and ground cover attributes), and (4) management (land use and grazing history). Broad phylogenetic structure was evident, but on a scale larger than our study area. Lizards were sexually dimorphic, whereby females had longer snout-vent length than males, providing support for the fecundity selection hypothesis. Body size variation in M. boulengeri was correlated with temperature and rainfall, a pattern consistent with larger individuals occupying cooler and more productive parts of the landscape. Climate change forecasts, which predict warmer temperature and increased aridity, may result in reduced lizard biomass and decoupling of trophic interactions with potential implications for community organization and ecosystem function.

Unintended consequences of invasive predator control in an Australian forest: overabundant wallabies and vegetation change.

Over-abundance of native herbivores is a problem in many forests worldwide. The abundance of native macropod wallabies is extremely high at Booderee National Park (BNP) in south-eastern Australia. This has occurred because of the reduction of exotic predators through an intensive baiting program, coupled with the absence of other predators. The high density of wallabies at BNP may be inhibiting the recruitment of many plant species following fire-induced recruitment events. We experimentally examined the post-fire response of a range of plant species to browsing by wallabies in a forest heavily infested with the invasive species, bitou bush Chrysanthemoides monilifera. We recorded the abundance and size of a range of plant species in 18 unfenced (browsed) and 16 fenced (unbrowsed) plots. We found the abundance and size of bitou bush was suppressed in browsed plots compared to unbrowsed plots. Regenerating seedlings of the canopy or middle storey tree species Eucalyptus pilularis, Acacia implexa, Allocasuarina littoralis, Breynia oblongifolia and Banksia integrifolia were either smaller or fewer in number in grazed plots than treatment plots as were the vines Kennedia rubicunda, Glycine tabacina and Glycine clandestina. In contrast, the understorey fern, Pteridium esculentum increased in abundance in the browsed plots relative to unbrowsed plots probably because of reduced competition with more palatable angiosperms. Twelve months after plots were installed the community structure of the browsed and unbrowsed plots was significantly different (P = 0.023, Global R = 0.091). The relative abundance of C. monilifera and P. esculentum contributed most to the differences. We discuss the possible development of a low diversity bracken fern parkland in Booderee National Park through a trophic cascade, similar to that caused by overabundant deer in the northern hemisphere. We also discuss its implications for broad scale fox control in southern Australian forests.

The combined effects of remnant vegetation and tree planting on farmland birds.

Biodiversity conservation on agricultural land is a major issue worldwide. We estimated separate and joint effects of remnant native woodland vegetation and recent tree plantings on birds on farms (approximately 500-1000 ha) in the heavily cleared wheat and sheep belt of southern Australia. Much of the variation (>70%) in bird responses was explained by 3 factors: remnant native-vegetation attributes (native grassland, scattered paddock trees, patches of remnant native woodland); presence or absence of planted native trees; and the size and shape of tree plantings. In terms of the number of species, remnant native vegetation was more important than tree planting, in a 3:1 ratio, approximately. Farms with high values for remnant native vegetation were those most likely to support declining or vulnerable species, although some individual species of conservation concern occurred on farms with large plantings. Farm management for improved bird conservation should account for the cumulative and complementary contributions of many components of remnant native-vegetation cover (e.g., scattered paddock trees and fallen timber) as well as areas of restored native vegetation.

Testing hypotheses associated with bird responses to wildfire.

Disturbance is a key ecological process influencing the distribution and abundance of many elements of the earth's biota. Predicting the response of biota to disturbance is therefore important, but it nevertheless remains difficult to make accurate forecasts of response. We tested predictions from disturbance-related theories and concepts in 10 vegetation types at Booderee National Park (southeastern Australia) using a retrospective study of bird responses to fire history (over 35 years) on 110 sites and a prospective study following a single wildfire event in 2003 at 59 of these sites. Our data did not support predictions from the intermediate-disturbance hypothesis; observed bird species richness at a site was significantly (F(1,99) = 6.30, P = 0.014) negatively related to the number of fires since 1972 and was 8.7% lower (95% CI, 1.8-15.1%) for each additional fire. In contrast to fire history effects, we found that after the 2003 fire, the vast majority of individual species and the bird assemblage per se in most vegetation types recovered within two years. Thus, recovery after a single fire did not reflect long-term effects of multiple fires on overall bird species richness at a site. We postulated that the recovery of bird species richness and bird assemblage composition after the 2003 fire would be fastest in structurally simple vegetation types and slowest in structurally complex vegetation, but observed the opposite. Although observed bird species richness in vertically heterogeneous forest and woodland had returned to prefire levels by 2006, bird species richness in structurally simple vegetation types (e.g., sedgeland) had not. Postfire vegetation regeneration, together with a paucity of early-successional specialists, would explain the speed of recovery of the bird assemblage and why it changed relatively little during our investigation.

Reptile and arboreal marsupial response to replanted vegetation in agricultural landscapes.

We report reptile and arboreal marsupial responses to vegetation planting and remnant native vegetation in agricultural landscapes in southeastern Australia. We used a hierarchical survey to select 23 landscapes that varied in the amounts of remnant native vegetation and planted native vegetation. We selected two farms within each landscape. In landscapes with plantings, we selected one farm with and one farm without plantings. We surveyed arboreal marsupials and reptiles on four sites on each farm that encompassed four vegetation types (plantings 7-20 years old, old-growth woodland, naturally occurring seedling regrowth woodland, and coppice [i.e., multistemmed] regrowth woodland). Reptiles and arboreal marsupials were less likely to occur on farms and in landscapes with comparatively large areas of plantings. Such farms and landscapes had less native vegetation, fewer paddock trees, and less woody debris within those areas of natural vegetation. The relatively large area of planting on these farms was insufficient to overcome the lack of these key structural attributes. Old-growth woodland, coppice regrowth, seedling regrowth, and planted areas had different habitat values for different reptiles and arboreal marsupials. We conclude that, although plantings may improve habitat conditions for some taxa, they may not effectively offset the negative effects of native vegetation clearing for all species, especially those reliant on old-growth woodland. Restoring suitable habitat for such species may take decades to centuries.

A graphical user interface for an electron monitor unit calculator using a sector-integration algorithm and exponential curve-fitting method.

A new electron monitor unit (MU) calculator program called "eMUc" was developed to provide a convenient electron MU calculation platform for the physics and radiotherapy staff in electron radiotherapy. The program was written using the Microsoft Visual Basic.net framework and has a user-friendly front-end window with the following features: (1) Apart from using the well-known polynomial curvefitting method for the interpolation and extrapolation of relative output factors (ROFs), an exponential curve-fitting method was used to obtain better results. (2) A new algorithm was used to acquire the radius in each angular segment in the irregular electron field during the sector integration. (3) A comprehensive graphical user interface running on the Microsoft Windows operating system was used. (4) Importing irregular electron cutout field images to the calculator program was simplified by using only a commercial optical scanner. (5) Interlocks were provided when the input patient treatment parameters could not be handled by the calculator database accurately. (6) A patient treatment record could be printed out as an electronic file or hard copy and transferred to the patient database. The data acquisition mainly required ROF measurements using various circular cutouts for all the available electron energies and applicators for our Varian 21 EX linear accelerator. To verify and implement the calculator, the measured results using our specific designed irregular and clinical cutouts were compared to those predicted by the calculator. Both agreed well with an error of +/-2%.