RESUMO
A fast capillary gas chromatographic method with nitrogen-selective detection is described that allows selective and reproducible quantification of n-butyl-p-aminobenzoate (BAB) and lidocaine in plasma. The sampling and sample storage conditions are critical for the quantification of BAB. Diisopropyl fluorophosphate, an organo-phosphorus pesticide, has to be added during sampling to prevent the rapid decomposition of BAB by cholinesterases.
Assuntos
Anestésicos Locais/sangue , Benzocaína/análogos & derivados , Cromatografia Gasosa , Lidocaína/sangue , Anestésicos Locais/administração & dosagem , Animais , Benzocaína/administração & dosagem , Benzocaína/sangue , Benzocaína/farmacocinética , Coleta de Amostras Sanguíneas , Calibragem , Inibidores da Colinesterase/farmacologia , Cães , Estabilidade de Medicamentos , Humanos , Injeções Epidurais , Isoflurofato/farmacologia , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
The conditions for the preparation of a 10% butyl-p-aminobenzoate suspension for epidural administration were investigated. Pharmaceutically acceptable suspensions are composed of butyl-p-aminobenzoate particles dispersed in a solvent consisting of the surfactant polysorbate 80 added to normal saline in a concentration of 0.25 mg/ml. pH Correction is not necessary. The suspensions are sterilized at 120 degrees C followed by special milling procedures to accomplish acceptable particle size. Butyl-p-aminobenzoate suspensions are stable at 4 degrees C during a period of at least four weeks.
