Effects of UV light on physicochemical changes in thermoplastic polyurethanes: Mechanism and disinfection byproduct formation.

The presence of microplastics (MPs) products and particles in the environment can significantly impact the human body. Most MPs that enter the environment also enter the water cycle. During sunlight light irradiation (especially ultraviolet (UV) part) or UV disinfection, many of these MPs, particularly those rich in surface functional groups like thermoplastic polyurethanes (TPU), undergo physicochemical changes that can affect the formation of disinfection byproducts (DBPs). This study investigates the physicochemical changes of TPU in water after exposure to UV irradiation and incubation in the dark, as well as the formation of DBPs after chlorination. The results show that TPU undergo chain breakage, oxidation, and cross-linking when exposed to UV irradiation in an aqueous system. This leads to fragmentation into smaller particles, which facilitates the synthesis of DBPs. Subsequent research has demonstrated that the TPU leaching solution produces a significantly higher DBP content than the chlorination of TPU MPs, particularly at high concentrations of CHCl3. Therefore, it is important to give greater consideration to the soluble DBP precursors released by TPU.

Disentangling microbial niche balance and intermediates' trade-offs for anaerobic digestion stability and regulation.

Anaerobic digestion (AD) is a key technology for converting organic matters to methane-rich biogas. However, nutrient imbalance can destabilize the whole digestion. To realize stable operation of AD and improve its efficiency, this work considers a new strategy to control the intermediate concentrations of poor AD under nutrient stress. For this purpose, long-term digestion under different nutrient conditions was investigated. Results showed that the feedstock with a low C/N ratio (= 6) caused VFA accumulation (2072 ± 632 mg/L), leading to the inhibition of methane production. Employing a substrate with a higher C/N ratio (= 11) and/or adding NH4HCO3 (200 mg NH4+-N/Ladd) could alleviate the VFA inhibition, but excessive dosage of NH4HCO3 would induce ammonia inhibition. Through the established digestion balance between free ammonia nitrogen (FAN) between 0 and 25 mg/L, volatile fatty acid (VFA) 510-2100 mg/L, and alkalinity (ALK) 3300-7800 mg/L, an efficient methane yield of 150-250 mL/g VS was achieved and stable operation of AD under nutrient stress (low C/N ratio) was realized. Metabolic reconstruction between Euryarchaeota sp. MAG162, Methanosarcina mazei MAG53 and Mesotoga infera MAG119 highlighted that microbial niche balance was developed as a result of digestion balance, which is beneficial for stable operation of AD. These findings improved our understanding of the interaction mechanism between intermediates and microbial niches for stability control in AD.

Evaluation of cell disruption methods for protein and coenzyme Q10 quantification in purple non-sulfur bacteria.

A recent focus has been on the recovery of single-cell protein and other nutritionally valuable bioproducts, such as Coenzyme Q10 (CoQ10) from purple non-sulfur bacteria (PNSB) biomass following wastewater treatment. However, due to PNSB's peculiar cell envelope (e.g., increased membrane cross-section for energy transduction) and relatively smaller cell size compared to well-studied microbial protein sources like yeast and microalgae, the effectiveness of common cell disruption methods for protein quantification from PNSB may differ. Thus, this study examines the efficiency of selected chemical (NaOH and EDTA), mechanical (homogenization and bead milling), physical (thermal and bath/probe sonication), and combined chemical-mechanical/physical treatment techniques on the PNSB cell lysis. PNSB biomass was recovered from the treatment of gas-to-liquid process water. Biomass protein and CoQ10 contents were quantified based on extraction efficiency. Considering single-treatment techniques, bead milling resulted in the best protein yields (p < 0.001), with the other techniques resulting in poor yields. However, the NaOH-assisted sonication (combined chemical/physical treatment technique) resulted in similar protein recovery (p = 1.00) with bead milling, with the former having a better amino acid profile. For example, close to 50% of the amino acids, such as sensitive ones like tryptophan, threonine, cystine, and methionine, were detected in higher concentrations in NaOH-assisted sonication (>10% relative difference) compared to bead-milling due to its less disruptive nature and improved solubility of amino acids in alkaline conditions. Overall, PNSB required more intensive protein extraction techniques than were reported to be effective on other single-cell organisms. NaOH was the preferred chemical for chemical-aided mechanical/physical extraction as EDTA was observed to interfere with the Lowry protein kit, resulting in significantly lower concentrations. However, EDTA was the preferred chemical agent for CoQ10 extraction and quantification. CoQ10 extraction efficiency was also suspected to be adversely influenced by pH and temperature.

Corrigendum to Comparative computational study to augment UbiA prenyltransferases inherent in purple photosynthetic bacteria cultured from mangrove microbial mats in Qatar for coenzyme Q10 biosynthesis: [Biotechnology Reports 36 (2022) e00775].

[This corrects the article DOI: 10.1016/j.btre.2022.e00775.].

Nutrient treatment of greywater in green wall systems: A critical review of removal mechanisms, performance efficiencies and system design parameters.

Greywater has lower pathogen and nutrient levels than other mixed wastewaters, making it easier to treat and to reuse in nature-based wastewater treatment systems. Green walls (GWs) are one type of nature-based solutions (NBS) that are evolving in design to support on-site and low-cost greywater treatment. Greywater treatment in GWs involves interacting and complex physical, chemical, and biological processes. Design and operational considerations of such green technologies must facilitate these pivotal processes to achieve effective greywater treatment. This critical review comprehensively analyses the scientific literature on nutrient removal from greywater in GWs. It discusses nutrient removal efficiency in different GW types. Total nitrogen removal ranges from 7 to 91% in indirect green facades (IGF), 48-93% for modular living walls (MLW), and 8-26% for continuous living walls (CLW). Total phosphorus removal ranges from 7 to 67% for IGF and 2-53% for MLW. The review also discusses the specific nutrient removal mechanisms orchestrated by vegetation, substrates, and biofilms to understand their role in nitrogen and phosphorus removal within GWs. The effects of key GW design parameters on nutrient removal, including substrate characteristics, vegetation species, biodegradation, temperature, and operating parameters such as irrigation cycle and hydraulic loading rate, are assessed. Results show that greater substrate depth enhances nutrient removal efficiency in GWs by facilitating efficient filtration, straining, adsorption, and various biological processes at varying depths. Particle size and pore size are critical substrate characteristics in GWs. They can significantly impact the effectiveness of physicochemical and biological removal processes by providing sufficient pollutant contact time, active surface area, and by influencing saturation and redox conditions. Hydraulic loading rate (HLR) also impacts the contact time and redox conditions. An HLR between 50 and 60 mm/d during the vegetation growing season provides optimal nutrient removal. Furthermore, nutrient removal was higher when watering cycles were customized to specific vegetation types and their drought tolerances.

Adsorption of Methyl Orange from Water Using Chitosan Bead-like Materials.

Natural product waste treatment and the removal of harmful dyes from water by adsorption are two of the crucial environmental issues at present. Traditional adsorbents are often not capable in removing detrimental dyes from wastewater due to their hydrophilic nature and because they form strong bonds with water molecules, and therefore they remain in the dissolved state in water. Consequently, new and effective sorbents are required to reduce the cost of wastewater treatment as well as to mitigate the health problems caused by water pollution contaminants. In this study, the adsorption behaviour of methyl orange, MO, dye on chitosan bead-like materials was investigated as a function of shaking time, contact time, adsorbent dosage, initial MO concentration, temperature and solution pH. The structural and chemical properties of chitosan bead-like materials were studied using several techniques including SEM, BET, XRD and FTIR. The adsorption process of methyl orange by chitosan bead materials was well described by the Langmuir isotherm model for the uptake capacity and followed by the pseudo-second-order kinetic model to describe the rate processes. Under the optimal conditions, the maximum removal rate (98.9%) and adsorption capacity (12.46 mg/g) of chitosan bead-like materials were higher than those of other previous reports; their removal rate for methyl orange was still up to 87.2% after three regenerative cycles. Hence, this chitosan bead-like materials are very promising materials for wastewater treatment.

Comparative computational study to augment UbiA prenyltransferases inherent in purple photosynthetic bacteria cultured from mangrove microbial mats in Qatar for coenzyme Q10 biosynthesis.

Coenzyme Q10 (CoQ10) is a powerful antioxidant with a myriad of applications in healthcare and cosmetic industries. The most effective route of CoQ10 production is microbial biosynthesis. In this study, four CoQ10 biosynthesizing purple photosynthetic bacteria: Rhodobacter blasticus, Rhodovulum adriaticum, Afifella pfennigii and Rhodovulum marinum, were identified using 16S rRNA sequencing of enriched microbial mat samples obtained from Purple Island mangroves (Qatar). The membrane bound enzyme 4-hydroxybenzoate octaprenyltransferase (UbiA) is pivotal for bacterial biosynthesis of CoQ10. The identified bacteria could be inducted as efficient industrial bio-synthesizers of CoQ10 by engineering their UbiA enzymes. Therefore, the mutation sites and substitution residues for potential functional enhancement were determined by comparative computational study. Two mutation sites were identified within the two conserved Asp-rich motifs, and the effect of proposed mutations in substrate binding affinity of the UbiA enzymes was assessed using multiple ligand simultaneous docking (MLSD) studies, as a groundwork for experimental studies.

A review of prospects and current scenarios of biomass co-pyrolysis for water treatment.

With ever-growing population comes an increase in waste and wastewater generated. There is ongoing research to not only reduce the waste but also to increase its value commercially. One method is pyrolysis, a process that converts wastes, at temperatures usually above 300 °C in a pyrolysis unit, to carbon-rich biochars among with other useful products. These chars are known to be beneficial as they can be used for water treatment applications; certain studies also reveal improvements in the biochar quality especially on the surface area and pore volume by imparting thermal and chemical activation methods, which eventually improves the uptake of pollutants during the removal of inorganic and organic contaminants in water. Research based on single waste valorisation into biochar applications for water treatment has been extended and applied to the pyrolysis of two or more feedstocks, termed co-pyrolysis, and its implementation for water treatment. The co-pyrolysis research mainly covers activation, applications, predictive calculations, and modelling studies, including isotherm, kinetic, and thermodynamic adsorption analyses. This paper focuses on the copyrolysis biochar production studies for activated adsorbents, adsorption mechanisms, pollutant removal capacities, regeneration, and real water treatment studies to understand the implementation of these co-pyrolyzed chars in water treatment applications. Finally, some prospects to identify the future progress and opportunities in this area of research are also described. This review provides a way to manage solid waste in a sustainable manner, while developing materials that can be utilized for water treatment, providing a double target approach to pollution management.

A critical overview of MXenes adsorption behavior toward heavy metals.

In recent years, tremendous interest has been generated in MXenes as a fast-growing and diversified family of two-dimensional (2D) materials with a wide range of potential uses. MXenes exhibit many unique structural and physicochemical properties that make them particularly attractive as adsorbents for removing heavy metals from aqueous media, including a large surface area, abundant surface terminations, electron-richness, and hydrophilic nature. In light of the adsorption capabilities of MXenes at the ever-increasing rate of expansion, this review investigates the recent computational predictions for the adsorption capabilities of MXenes and the effect of synthesis of different MXene on their remediation behavior toward heavy metals. The influence of MXene engineering strategies such as alkalization, acidification, and incorporation into organic and inorganic hosts on their surface properties and adsorption capacity is compared to provide critical insights for designing effective MXene adsorbents. Additionally, the review discusses MXenes' adsorption mechanisms, the effect of coexisting ions on MXenes' selectivity, the regeneration of exhausted MXenes, and provides an overview of MXenes' stability and biocompatibility to demonstrate their potentiality for wastewater remediation. Finally, the review identifies current flaws and offers recommendations for further research.

Can a compact biological system be used for real hydraulic fracturing wastewater treatment?

Hydraulic fracturing wastewater (HFW), a byproduct of hydraulic fracturing oil extraction, contains a complex mixture of oil, aldehydes, and benzene compounds. Efficient and eco-friendly HFW treatment means are critical for the oil extraction industry, particularly in developing countries. In this study, two biological processes namely an anaerobic/anoxic/moving bed biofilm reactor (A2-MBBR) and an A2-MBBR with a microfiltration membrane (A2-MFMBBR) were established, and assessed for the real HFW treatment. Removal efficiencies of chemical oxygen demand (COD) and NH4+-N were over 92% and 95%, respectively, in both processes with a hydraulic retention time of 72 h. The majority of organic compounds in both systems identified by GC-MS were degraded in the anaerobic units. In comparison, A2-MFMBBR demonstrated higher removal efficiencies for oil, total suspended solids, and complex compounds. The average relative abundances of refractory compound degrading bacteria were 43.4% and 51.6% in the A2-MBBR and A2-MFMBBR, respectively, which was consistent with the COD and oil removal, and suggested that the MBR could maintain a high diversity of microorganisms and contribute to deep recalcitrant organics degradation. This study sheds light on the potential of using a compact biological process for the real HFW treatment.

Efficient Photocatalytic Degradation of Organic Dyes by AgNPs/TiO2/Ti3C2T x MXene Composites under UV and Solar Light.

Due to their broad applications in various industrial activities, and their well-known negative impacts on the aquatic environment, organic dyes have been continuously identified as serious threat to the quality of ecosystems. The photocatalytic degradation process in aqueous solutions has emerged as an efficient and reliable approach for the removal of organic dyes. MXenes, a new class of two-dimensional (2D) nanomaterials, possess unique chemical composition, surface functionalities, and physicochemical properties. Such characteristics enable MXenes to act as efficient catalysts or cocatalysts to photodegrade organic molecules. This work explores the application of Ti3C2T x MXene decorated with silver and palladium nanoparticles, using a simple hydrothermal treatment method, for the photocatalytic degradation of methylene blue (MB) and rhodamine B (RhB). The chemical composition of these photocatalysts, as well as their structural properties and morphology, was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS) techniques. The photocatalytic degradation abilities of the pristine MXene and the synthesized MXene composites were investigated under ultraviolet and solar light irradiation. A significant improvement in the photocatalytic performances was observed for all oxidized MXene composites when compared to pristine MXene, with a superior degradation efficiency achieved for AgNPs/TiO2/Ti3C2T x . This work broadens the application range of oxidized MXene composites, providing an alternative material for degrading organics dyes and wastewater treatment applications.

The effectiveness of divalent cation addition for highly saline activated sludge cultures: Influence of monovalent/divalent ratio and specific cations.

Saline wastewaters are prevalent in various industries and pose challenges to stable biological treatment. Increasing monovalent cation concentrations are commonly reported to deteriorate treatment and settling performance, while divalent cations can enhance flocculation and settling. However, many previous studies were performed at relatively low salinities and reports conflict on whether concentrations of monovalent cations, divalent cations, or their ratio (M/D) are most critical. This study investigates whether addition of divalent cations shows the same benefits at high salinity (∼40 g NaCl.L-1) and whether divalent ion concentration or M/D is a better predictor of enhancement. Nine sequencing batch reactors were operated at 0.8 M NaCl or KCl monovalent salt concentration, and the concentration of divalent cations (Ca2+ and Mg2+) was varied. M/D was found to be the critical factor that consistently influenced sludge characteristics. It was particularly important in describing hydrophobicity, sludge volume index (SVI) and specific oxygen uptake rate (SOUR), with rpartial of -0.879, 0.971 and 0.966 respectively in models that had an r2adj greater than 0.93. Lower M/D also increased biomass concentrations and reduced extracellular polysaccharides, the latter which in turn correlated strongly with many shape and surface charge measures. The specific monovalent salt (Na+ or K+) influenced treatment performance, biomass concentrations, hydrophobicity, SOUR, extracellular protein and SVI. The specific divalent cation was only important in describing SVI, where Mg2+ was beneficial. Overall, this study shows that addition of divalent cations can greatly benefit high salinity activated sludge systems by improving the sludge structure, settling and organic removal.

Thermal degradation characteristics and gasification kinetics of camel manure using thermogravimetric analysis.

In this work, the sustainable valorisation of camel manure has been studied using thermogravimetric analysis. The gasification tests were performed from ambient conditions to 950 °C at 10, 20, and 50 °C/min under an O2 environment. The TGA data were applied to determine the kinetics of the O2 gasification. Single-heating rate models (Arrhenius and Coats-Redfern) and multi-heating rate models (Distributed activation energy, Friedman, Flynn-Wall-Ozawa, Starink, and Kissinger-Akahira-Sunose) were applied to estimate the kinetics of the process. Between the two single-heating rate models, the Coats-Redfern method fitted best with the experimental data. Among the multi-heating rate models, the Flynn-Wall-Ozawa model fitted best with the experimental results. The kinetic parameters-frequency factor, activation energy, and order of reaction were estimated using the Flynn-Wall-Ozawa model (the best-fitting model) and the estimated kinetic parameters were used to calculate the thermodynamic properties-Gibbs free energy, enthalpy, and entropy. The information on these kinetic and thermodynamic properties can be useful for the design of gasifiers and for optimising the O2 gasification operating conditions.

The impact of pyrolysis conditions on orange peel biochar physicochemical properties for sandy soil.

The citrus industry is considered one of the main contributors to agricultural waste. Peels are commonly used in the food industry or as feedstock in biorefining. In this study, the potential of waste orange peel biochar for agricultural applications in sandy soil was investigated. This will not only increase the percentage of agricultural waste recycling, but also lead to more sustainable agriculture with environmental benefits such as carbon sequestration. Biochar was produced through slow pyrolysis in the temperature range 300-600°C and at two holding durations (10 min and 60 min). Both factors had a significant impact on the physicochemical characteristics of biochar in the heating region 300-450°C. However, varying the holding time for pyrolysis temperatures beyond 450°C had a diminishing effect on biochar properties compared with the impact of increasing pyrolysis temperature. The study also looked at certain properties that are specific to agricultural application not previously reported for orange peel. Very high cation exchange capacities of 70 cmol kg-1 were achieved at 300°C, whereas water holding capacity was not strongly influenced by pyrolysis conditions. Preliminary planting tests indicate potential for improving agricultural sustainability in sandy soils. The technoeconomic analysis of biochar showed that the pyrolysis process can be profitable with sufficient plant capacity.

Shock effects of monovalent cationic salts on seawater cultivated granular sludge.

Saline wastewater is commonly encountered in various industries, posing challenges to biological treatments. The application of seawater as a seed source provides a media of diverse halophilic organisms for rapid startup. However, effects of transitioning from a mixed salt source to monovalent salt solutions prevalent in industry remains unexplored. Hence, seed sludge was cultivated using seawater and later granulated under a mixed-salt synthetic medium comprising a mixture of NaCl, KCl and Na2SO4 at a combined concentration of 0.8 M (0.27 M each). The stable, acclimated granules were then tested against single salt media of 0.8 M NaCl, KCl, or Na2SO4. Shift to single salt media resulted in granule disaggregation, poor settling, sludge washout and development of fluffy or slimy flocs. Changes in exopolysaccharides composition after the single salt shift was the predominant reason for the large changes in sludge morphology. The impacts of KCl and Na2SO4 were more significant than the shift to NaCl. The resulting impacts also had a major influence on the treatment performance. A complex mechanism involving monovalent cation stimulation of proteins; ionic strength impacts on exopolysaccharides and morphology; solution density influence on sludge density and settling; and tonicity impacts on cell viability and treatment is described.

An overview of anoxygenic phototrophic bacteria and their applications in environmental biotechnology for sustainable Resource recovery.

Anoxygenic phototrophic bacteria (APB) are a phylogenetically diverse group of organisms that can harness solar energy for their growth and metabolism. These bacteria vary broadly in terms of their metabolism as well as the composition of their photosynthetic apparatus. Unlike oxygenic phototrophic bacteria such as algae and cyanobacteria, APB can use both organic and inorganic electron donors for light-dependent fixation of carbon dioxide without generating oxygen. Their versatile metabolism, ability to adapt in extreme conditions, low maintenance cost and high biomass yield make APB ideal for wastewater treatment, resource recovery and in the production of high value substances. This review highlights the advantages of APB over algae and cyanobacteria, and their applications in photo-bioelectrochemical systems, production of poly-ß-hydroxyalkanoates, single-cell protein, biofertilizers and pigments. The ecology of ABP, their distinguishing factors, various physiochemical parameters governing the production of high-value substances and future directions of APB utilization are also discussed.

Performance evaluation of various individual and mixed media for greywater treatment in vertical nature-based systems.

Nature-based systems (NBS) are a cost-effective, energy efficient and aesthetically pleasing approach for greywater treatment, but they are space intensive. Vertical NBS overcome this issue but must utilize lightweight media to reduce their construction costs. This study evaluates four common plant growing media: perlite, coco coir, LECA and sand, and compares them with two new media derived from local waste materials: date seeds and spent coffee grounds (SCG). The media are characterized and tested for their removal of various greywater pollutants. Further tests are conducted comparing mixtures of perlite-coco coir and date seeds-SCG. SCG was found to be an excellent media for greywater treatment, providing a similar degree of treatment as the best traditional media, coco coir and providing improved drainage. Drainage was further improved by mixing SCG with date seeds, which performed better than any mixture of perlite and coco coir. Most pollutants showed a slight deterioration in treatment performance with this mixture, although the removal of suspended solids and chemical oxygen demand was improved. An increased bed height improved the treatment performance with SCG, while increased hydraulic loading resulted in decreased treatment performance for all media. This study demonstrates the potential of date seeds and SCG as locally recycled waste materials to realize treatment of greywater in vertical NBS.

Food waste from a university campus in the Middle East: Drivers, composition, and resource recovery potential.

Food waste is a pressing issue that imposes economic, social and environmental impacts on both developing and developed countries. This study analyzes quantitatively and qualitatively the generated food waste at various food outlets of a university campus in Qatar. It is a fundamental step to manage the issue of food waste from educational institutes. The investigation comprised four stages: screening, sampling, surveying, and synthesis. Food waste generation at the sampled locations was estimated at 329.5 kg/day or 80 t/year. Based on per sales estimates, total food waste was 980 g/sale and 757 g/sale at the student male and female housing complexes, respectively, equating to roughly one wasted meal for each sold meal. The majority of this waste was avoidable waste and the root cause for the excessive food waste generation was overproduction rather than consumer wastage. The study found that the main food provider, who primarily serves buffet style meals, lacks the proper tools to measure food waste generated at their cafeterias. Past experience was the primary tool to support the company's demand management estimation which has proven unsuccessful and highlights the need to not only educate the consumer but also food providers. Possible treatments routes are discussed based on food waste characterization findings.

Effect of Graphene Oxide Synthesis Method on Properties and Performance of Polysulfone-Graphene Oxide Mixed Matrix Membranes.

Graphene oxide (GO) has shown great promise as a nanofiller to enhance the performance of mixed matrix composite membranes (MMMs) for water treatment applications. However, GO can be prepared by various synthesis routes, leading to different concentrations of the attached oxygen functional groups. In this research, GO produced by the Hummers', Tour, and Staudenmaier methods were characterized and embedded at various fractions into the matrix of polysulfone (PSf) and used to prepare microfiltration membranes via the phase inversion process. The effects of the GO preparation method and loading on the membrane characteristics, as well as performance for oil removal from an oil-water emulsion, are analyzed. Our results reveal that GO prepared by the Staudenmaier method has a higher concentration of the more polar carbonyl group, increasing the membrane hydrophilicity and porosity compared to GO prepared by the Hummers' and Tour methods. On the other hand, the Hummers' and Tour methods produce GO with larger sheet size, and are more effective in enhancing the mechanical properties of the PSf membrane. Finally, all MMMs exhibited improved water flux (up to 2.7 times) and oil rejection, than those for the control PSf sample, with the optimum GO loading ranged between 0.1-0.2 wt%.

Environmental assessment of intake alternatives for seawater reverse osmosis in the Arabian Gulf.

The study carried out an environmental assessment for two seawater reverse osmosis (RO) plants located within the Arabian Gulf considering subsurface intake alternatives and differing energy source options. The study used life cycle assessment to quantify the environmental impacts for open intake pretreatment vs. subsurface intake pretreatments of two plants with operating capacities of approximately 175,000 m3/d and 275,000 m3/d respectively. For both RO plants, electricity and chemical inputs were considered. Significant energy reductions of 30% were observed with subsurface intakes for extraction and pretreatment, resulting in a plant-wide energy saving of 6%. Open intake pretreatment had higher environmental impacts compared to subsurface intake across all impact categories, although in some impact categories significant differences existed between the two similar plants due to differences in chemicals used. The study further established that the renewable PV power generation resulted in the lowest global warming potential (GWP); however, a significant trade-off occurs with this energy source since it had the highest impact relative to both ozone and abiotic depletion potentials and was also worse than natural gas for both marine and human toxicity potentials. The GWP reductions achievable using a subsurface intake for the larger of the two plants is equivalent to 58,000 tons of CO2 per year, or more than 12,000 cars, making subsurface intakes a worthy alternative to conventional open intake systems in the Arabian Gulf region. Marine aquatic eco-toxicity potential was identified as the most significant normalized environmental impact, which should be best managed through using natural gas as an energy source.