[Antigenic properties of concentrated antirabies vaccine in accelerated immunization schedules]. / Antigennye svoistva kontsentrirovannoi antirabicheskoi vaktsiny pri ukorochennykh skhemakh immunizatsii.

The study of purified rabies vaccine (tissue culture origin), concentrated 100 fold, in animal experiments has shown that the preparation, if introduced intramuscularly in 2 or 3 injections, has high antigenic activity. The intramuscular injections of the vaccine at an interval of 21 days or at intervals of 21 and 30 days after the first injection has proved to be the optimum immunization schedule ensuring a sufficiently high accumulation of virus-neutralizing antibodies by days 30--45. To achieve higher antibody titers, the vaccine may be introduced intradermally after 2 indispensable intramuscular injections of the preparation.

[Serological method of detecting antirabies antibodies]. / Serologicheskii metod obnaruzheniia antirabicheskikh antitel.

The possibility of the determination of rabies antibodies by the serological method has been demonstrated, involving the use of culture rabies virus, strain Vnukovo-32, concentrated and purified by high speed centrifugation, for the sensitization of anserine erythrocytes. The neutralization test in mice and the passive hemagglutination test showed a correlation in rabies antibody titers in the sera of animlas immunized with rabies vaccines. The advantages of the passive hemagglutination test consist in the rapidity of obtaining the results, in a simple and economic method of carrying out the test.

[Study of methods of inactivating a concentrated, purified cultured antirabies vaccine]. / Izuchenie metodov inaktivatsii kontsentrirovannoi ochishchennoi kul'tural'noi antirabicheskoi vaktsiny.

Two methods of inactivation, with ultraviolet (UV) and gamma (gamma) rays, of the concentrated purified tissue culture rabies vaccine from the Vnukovo-32 strain were studied. Under the optimal conditions of treatment of 100-fold concentrated purified virus-containing suspension, a completely inactivated vaccine with high immunogenicity indices (4.1 to 78) was obtained. Both the inactivation methods were found to be of equal value.