RESUMO
This new reagent kit for the quantitative measurement of oxalate in urine is a modification of an earlier Sigma oxalate assay procedure (procedure no. 590), a coupled enzyme assay involving oxalate oxidase and horseradish peroxidase. The new analytical procedure includes methods for processing urine specimens to eliminate interference with oxalate color development at 590 nm by ascorbic acid, divalent cations, and other urinary constituents. The reaction is complete in less than 5 min, and results are linearly related to oxalate concentration up to at least 1 mmol/L. Assay sensitivity and within-run and between-run precision were within the limits acceptable for other urinary oxalate procedures. Analytical recovery of added oxalate was close to 100%. This specific, simple, rapid procedure is suitable for routine clinical use.
Assuntos
Peroxidase do Rábano Silvestre , Oxalatos/urina , Oxirredutases , Peroxidases , Ácido Ascórbico , Cálcio , Reações Falso-Negativas , Humanos , Oxalatos/normas , Kit de Reagentes para Diagnóstico , Estatística como AssuntoAssuntos
Líquido Amniótico/análise , Sangue Fetal/análise , alfa-Fetoproteínas/análise , Aminoácidos/análise , Amino Açúcares/análise , Animais , Carboidratos/análise , Eletroforese em Gel de Poliacrilamida , Idade Gestacional , Imunoeletroforese , Ponto Isoelétrico , Camundongos , Camundongos Endogâmicos A , Camundongos Endogâmicos C3H , Peso Molecular , Neuraminidase , Ácidos Siálicos/análise , alfa-Fetoproteínas/isolamento & purificaçãoAssuntos
Fígado/enzimologia , Sialiltransferases/metabolismo , Transferases/metabolismo , Animais , Ácido Edético/farmacologia , Eletroforese em Gel de Poliacrilamida , Feminino , Feto , Idade Gestacional , Fígado/efeitos dos fármacos , Camundongos , Fosfolipases/farmacologia , Gravidez , Tripsina/farmacologia , Zinco/farmacologiaRESUMO
The temporal accumulation of the electrophoretic components of mouse alpha-foetoprotein in foetal plasma and amniotic fluid is reported. To explain the progressive appearance of the sialylated alpha-foetoproteins, the activity of sialyltransferase in foetal liver and yolk sac was measured. These results indicate that the increase in sialyltransferase activity in these tissues is responsible for the increased sialylation of alpha-foetoprotein.